Date published: 2025-9-6
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BAF Antibody (A-11) - Western Blotting - Image 373602
BAF 抗体 (A-11): sc-166324. NIH/3T3 (A), PC-12 (B), Sol8 (C) 和 SP2/0 (D) 全细胞裂解液中 BAF 表达的 Western 印迹分析.
BAF 抗体 (A-11): sc-166324. NIH/3T3 (A), PC-12 (B), Sol8 (C) 和 SP2/0 (D) 全细胞裂解液中 BAF 表达的 Western 印迹分析.

BAF 抗体 (A-11): sc-166324

4.1(7)
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说明书
  • BAF 抗体 A-11 是小鼠单克隆 IgG2a κ，BAF抗体, 在11篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1-89的抗原，代表全长 屏障-自整合因子human起源
  • BAF 抗体 (A-11) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 BAF; 也和以下物种反应，包括： 和 equine, canine and bovine
  • 抗BAF抗体（A-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-166324 X, 200 µg/0.1 ml)
  • 关于如何获取BAF (A-11): sc-166324的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是BAF Antibody (A-11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与BAF Antibody (A-11) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加BAF抗体，包括BAF155, BAF170, BAF53, BAF57和BAF60a

BAF抗体（A-11）是一种IgG2a κ小鼠单克隆BAF抗体（也称为BAF抗体），可通过WB、IP、IF和ELISA方法检测小鼠、大鼠和人源的BAF蛋白。BAF抗体（A-11）既可以是非偶联的抗BAF抗体形式，也可以是多种偶联形式的抗BAF抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。屏障-自整合因子(BAF)非特异性地结合双链DNA，可能通过与不同的同源结构域转录因子相互作用，在组织或细胞类型特异性基因表达中发挥作用。BAF压缩染色质结构并与核蛋白的LEM结构域相互作用，在核组装过程中膜募集和染色质去凝聚中发挥关键作用。此外，像HIV-1这样的逆转录病毒将从宿主细胞整合的BAF纳入预整合复合物(PICs)中，以防止逆转录病毒DNA的自整合，从而促进逆转录病毒DNA整合到宿主染色体中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

BAF 抗体 (A-11) 参考文献:

  1. emerin的不同功能域可结合层粘连蛋白A和DNA桥接蛋白BAF。  |  Lee, KK., et al. 2001. J Cell Sci. 114: 4567-73. PMID: 11792821
  2. 新生核素组装到重组核包膜中需要 BAF。  |  Haraguchi, T., et al. 2001. J Cell Sci. 114: 4575-85. PMID: 11792822
  3. 确定自体抗原 HB 为自体结合障碍因素。  |  Forné, I., et al. 2003. J Biol Chem. 278: 50641-4. PMID: 14523012
  4. 屏障-自整合因子 BAF 与 p55 Gag 和基质结合，是人类免疫缺陷病毒 1 型病毒的宿主成分。  |  Mansharamani, M., et al. 2003. J Virol. 77: 13084-92. PMID: 14645565
  5. 通过基于结构的 PROTAC 设计展示癌症中 BAF 复合物的脆弱性。  |  Farnaby, W., et al. 2019. Nat Chem Biol. 15: 672-680. PMID: 31178587
  6. 核糖体结合人类 BAF 复合物的结构。  |  He, S., et al. 2020. Science. 367: 875-881. PMID: 32001526
  7. 内源性人类 BAF 复合物结构模型为疾病机理提供信息  |  Mashtalir, N., et al. 2020. Cell. 183: 802-817.e24. PMID: 33053319
  8. BAF 染色质重塑复合物：结构、功能和合成致死性。  |  Varga, J., et al. 2021. Biochem Soc Trans. 49: 1489-1503. PMID: 34431497
  9. BAF复合物可维持小儿H3K27M胶质瘤干细胞的生长  |  Panditharatna, E., et al. 2022. Cancer Discov. 12: 2880-2905. PMID: 36305736

订购信息

产品名称产品编号规格价格数量收藏夹

BAF 抗体 (A-11)

sc-166324
200 µg/ml
$316.00

BAF (A-11): m-IgG Fc BP-HRP 套装

sc-528943
200 µg Ab; 10 µg BP
$354.00

BAF (A-11): m-IgGκ BP-HRP 套装

sc-521566
200 µg Ab, 40 µg BP
$354.00

BAF (A-11): m-IgG2a BP-HRP 套装

sc-547212
200 µg Ab; 10 µg BP
$354.00

BAF 抗体 (A-11) X

sc-166324 X
200 µg/0.1 ml
$316.00

BAF 抗体 (A-11) AC

sc-166324 AC
500 µg/ml, 25% agarose
$416.00

BAF 抗体 (A-11) HRP

sc-166324 HRP
200 µg/ml
$316.00

BAF 抗体 (A-11) FITC

sc-166324 FITC
200 µg/ml
$330.00

BAF 抗体 (A-11) PE

sc-166324 PE
200 µg/ml
$343.00

BAF 抗体 (A-11) Alexa Fluor® 488

sc-166324 AF488
200 µg/ml
$357.00

BAF 抗体 (A-11) Alexa Fluor® 546

sc-166324 AF546
200 µg/ml
$357.00

BAF 抗体 (A-11) Alexa Fluor® 594

sc-166324 AF594
200 µg/ml
$357.00

BAF 抗体 (A-11) Alexa Fluor® 647

sc-166324 AF647
200 µg/ml
$357.00

BAF 抗体 (A-11) Alexa Fluor® 680

sc-166324 AF680
200 µg/ml
$357.00

BAF 抗体 (A-11) Alexa Fluor® 790

sc-166324 AF790
200 µg/ml
$357.00

When should the TransCruz reagent sc-166324 X be used instead of the standard form sc-166324?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2019-03-09
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Rated 4 out of 5 by from Good in immunofluorescenceI've used this antibody in immunofluorescence and it performed very well on B95-8 cells (B cell lymphoma) fixed by acetone/methanol 1:1. Anti-Baf antibody was diluted 1:50.
Date published: 2019-11-12
Rated 5 out of 5 by from Great antibodyI used this antibody in WB and IF on U2OS and HEK human cell lines, and I obtained highly specific and clean signal with both methods.
Date published: 2018-08-13
Rated 1 out of 5 by from not suitable for immunofluorescenceAntibody was tested at 1:100 on formaldehyde-fixed HeLa cells and only produced cytoplasmic background. GFP-BAF was transfected as reference, staining for the ER protein REEP5 served as positive control for antibody staining.
Date published: 2018-02-07
Rated 4 out of 5 by from It worked.I used it in WB.1:200.The bands showed correct molecular weight.
Date published: 2017-11-23
Rated 5 out of 5 by from Excellent for WBit works excellent for WB in our lab, will keep usinig it
Date published: 2017-09-18
Rated 5 out of 5 by from Good BAFI use this antibody at a dilution of 1:3000 in TBS-Tween for 1hr and is enough for an good western blot signal. Loading of 10 ul of total samples is recommended for quantification.
Date published: 2016-11-24
Rated 5 out of 5 by from Produced nice Western blot data of BAF expressionProduced nice Western blot data of BAF expression in Jurkat whole cell lysate. -SCBT QC
Date published: 2013-05-14
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BAF 抗体 (A-11) is rated 4.1 out of 5 by 7.
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