Arnt 1 Antibody (H-6): sc-271801

Arnt 1 Antibody (H-6) is a mouse monoclonal IgG2a antibody that detects Arnt 1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Arnt 1, also known as aryl hydrocarbon receptor nuclear translocator, plays a crucial role in cellular signaling as Arnt 1 is essential for the translocation of the aryl hydrocarbon receptor (AhR) into the nucleus upon ligand binding. This process is vital for the regulation of genes involved in xenobiotic metabolism, including those encoding cytochrome P450 enzymes, which are responsible for the detoxification of harmful compounds. Arnt 1 consists of 789 amino acids and features a basic helix-loop-helix (bHLH) domain, a PAC (PAS-associated C-terminal) domain, and two PAS (PER-ARNT-SIM) domains, which are important for dimerization with AhR and subsequent binding to specific DNA sequences known as xenobiotic responsive elements (XREs). The interaction between Arnt 1 and AhR not only facilitates the transcriptional activation of target genes but also underscores Arnt 1′s significance in maintaining cellular homeostasis and responding to environmental toxins. Anti-Arnt 1 antibody (H-6) is an invaluable tool for researchers studying the mechanisms of gene regulation and the effects of environmental pollutants on cellular function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Arnt 1 Antibody (H-6) References:

1. Binding of the ERα and ARNT1 AF2 domains to exon 21 of the SRC1 isoform SRC1e is essential for estrogen- and dioxin-related transcription.  |  Endler, A., et al. 2012. J Cell Sci. 125: 2004-16. PMID: 22328528
2. MicroRNA 107 partly inhibits endothelial progenitor cells differentiation via HIF-1β.  |  Meng, S., et al. 2012. PLoS One. 7: e40323. PMID: 22792280
3. The Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT/HIF-1β) is influenced by hypoxia and hypoxia-mimetics.  |  Wolff, M., et al. 2013. Cell Physiol Biochem. 32: 849-58. PMID: 24081025
4. Coactivator recruitment of AhR/ARNT1.  |  Endler, A., et al. 2014. Int J Mol Sci. 15: 11100-10. PMID: 24950180
5. Expression of aryl hydrocarbon receptor 1 (AHR1), AHR1 nuclear translocator 1 (ARNT1) and CYP1 family monooxygenase mRNAs and their activity in chicken ovarian follicles following in vitro exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).  |  Antos, PA., et al. 2015. Toxicol Lett. 237: 100-11. PMID: 26043675
6. ARNT/HIF-1β links high-risk 1q21 gain and microenvironmental hypoxia to drug resistance and poor prognosis in multiple myeloma.  |  Wu, C., et al. 2018. Cancer Med. 7: 3899-3911. PMID: 29926531
7. Molecular cloning and characterization of two ARNT (ARNT-1 and ARNT-2) genes in Atlantic croaker and their expression during coexposure to hypoxia and PCB77.  |  Rahman, MS. and Thomas, P. 2019. Environ Toxicol. 34: 160-171. PMID: 30334616
8. HIF-1β Positively Regulates NF-κB Activity via Direct Control of TRAF6.  |  D'Ignazio, L., et al. 2020. Int J Mol Sci. 21: PMID: 32344511
9. Interaction between AhR and HIF-1 signaling pathways mediated by ARNT/HIF-1β.  |  Zhang, M., et al. 2022. BMC Pharmacol Toxicol. 23: 26. PMID: 35473600
10. DDX5 enhances HIF-1 activity by promoting the interaction of HIF-1α with HIF-1β and recruiting the resulting heterodimer to its target gene loci.  |  Shirai, Y., et al. 2024. Biol Cell. 116: e2300077. PMID: 38031929

The preferred Arnt 1 antibody is Arnt 1 Antibody (A-3): sc-17811.