Date published: 2025-9-6

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ARK-2 抗体 (A-3): sc-393357

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说明书
  • ARK-2 抗体 A-3 是小鼠单克隆 IgG2b κ,ARK-2抗体, 在3篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的ARK-2的内部区域内的氨基酸48-79之间
  • ARK-2 抗体 (A-3) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 ARK-2
  • 抗ARK-2抗体(A-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ARK-2 (A-3): sc-393357免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRP是ARK-2 Antibody (A-3) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与ARK-2 Antibody (A-3) 打包提供(请参阅下面的订购信息)。

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関連項目

ARK-2抗体(A-3)是一种IgG2b κ小鼠单克隆ARK-2抗体(也称为AURKB抗体、Aurora B激酶抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的ARK-2蛋白。ARK-2抗体(A-3)既可以是非偶联的抗ARK-2抗体形式,也可以是多种偶联形式的抗ARK-2抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。极光相关激酶-1(ARK-1、STK15、Aurora2、Aik1)和-2(ARK-2、STK12、Aurora1)是与中心体相关的丝氨酸/苏氨酸激酶,它们在有丝分裂过程中调节中心体分离、双极纺锤体组装和染色体分离。ARK-1和ARK-2在细胞核中表达,并在有丝分裂期间定位于有丝分裂机械的不同部分,如中心体、纺锤极(ARK-1)和中体(ARK-2)。ARK-1和ARK-2的转录本在人胸腺和胎儿肝脏中高水平存在。ARK-1蛋白在结肠癌细胞系(HT-29、SNU-C2B、COLO 205、SW480、837和948)中表达升高,并在HeLa细胞的中期累积。ARK-2蛋白水平在S期和G2/M期均达到最大,而ARK-1蛋白在G2/M期后通过泛素-蛋白酶体途径降解。ARK-2相对于ARK-1具有独特的基因位点,表明这两种具有致癌潜力的激酶在细胞周期进程中具有不同的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ARK-2 抗体 (A-3) 参考文献:

  1. Cdc20 与激酶 aurora2/Aik 结合。  |  Farruggio, DC., et al. 1999. Proc Natl Acad Sci U S A. 96: 7306-11. PMID: 10377410
  2. 极光/Ipl1p 相关激酶--有丝分裂丝氨酸-苏氨酸激酶的新致癌家族。  |  Giet, R. and Prigent, C. 1999. J Cell Sci. 112 (Pt 21): 3591-601. PMID: 10523496
  3. 通过无性繁殖促进复合体-泛素-蛋白酶体途径降解人类 Aurora2 蛋白激酶。  |  Honda, K., et al. 2000. Oncogene. 19: 2812-9. PMID: 10851084
  4. 基于极光激酶 B 的小鼠系统可有效识别和分析增殖的心肌细胞  |  Fu, W., et al. 2020. Front Cell Dev Biol. 8: 570252. PMID: 33117800
  5. 人视网膜母细胞瘤中极光激酶 B 的表达、调控和治疗靶点  |  Borah, NA., et al. 2021. Invest Ophthalmol Vis Sci. 62: 16. PMID: 33704359
  6. 极光激酶 B 抑制极光激酶 A,从而控制小鼠卵母细胞中母体 mRNA 的翻译。  |  Aboelenain, M. and Schindler, K. 2021. Development. 148: PMID: 34636397
  7. 极光激酶 B 在调节乳腺癌细胞对紫杉醇耐药性中的作用  |  Liu, M., et al. 2022. Hum Cell. 35: 678-693. PMID: 35088239
  8. 靶细胞BCL2克服小细胞肺癌患者对AZD2811抑制极光激酶B的耐药性并增强其反应  |  Ramkumar, K., et al. 2023. Clin Cancer Res. 29: 3237-3249. PMID: 37289191
  9. 哺乳动物极光同源物极光相关激酶(ARK)1 和 2 的 cDNA 克隆, 表达, 亚细胞定域和染色体分配。  |  Shindo, M., et al. 1998. Biochem Biophys Res Commun. 244: 285-92. PMID: 9514916
  10. 果蝇极光激酶的同源物在人类结直肠癌中具有致癌和扩增作用。  |  Bischoff, JR., et al. 1998. EMBO J. 17: 3052-65. PMID: 9606188
  11. 肿瘤扩增激酶 STK15/BTAK 可诱导中心粒扩增, 非整倍体和转化。  |  Zhou, H., et al. 1998. Nat Genet. 20: 189-93. PMID: 9771714
  12. STK12/Aik2:与果蝇极光和酵母 IPL1 有关的人类基因的鉴定和特征描述。  |  Kimura, M., et al. 1998. Cytogenet Cell Genet. 82: 147-52. PMID: 9858806

订购信息

产品名称产品编号规格价格数量收藏夹

ARK-2 抗体 (A-3)

sc-393357
200 µg/ml
$316.00

ARK-2 (A-3): m-IgG Fc BP-HRP 套装

sc-526242
200 µg Ab; 10 µg BP
$354.00

ARK-2 (A-3): m-IgG2b BP-HRP 套装

sc-549701
200 µg Ab; 10 µg BP
$354.00

ARK-2 抗体 (A-3) AC

sc-393357 AC
500 µg/ml, 25% agarose
$416.00

ARK-2 抗体 (A-3) HRP

sc-393357 HRP
200 µg/ml
$316.00

ARK-2 抗体 (A-3) FITC

sc-393357 FITC
200 µg/ml
$330.00

ARK-2 抗体 (A-3) PE

sc-393357 PE
200 µg/ml
$343.00

ARK-2 抗体 (A-3) Alexa Fluor® 488

sc-393357 AF488
200 µg/ml
$357.00

ARK-2 抗体 (A-3) Alexa Fluor® 546

sc-393357 AF546
200 µg/ml
$357.00

ARK-2 抗体 (A-3) Alexa Fluor® 594

sc-393357 AF594
200 µg/ml
$357.00

ARK-2 抗体 (A-3) Alexa Fluor® 647

sc-393357 AF647
200 µg/ml
$357.00

ARK-2 抗体 (A-3) Alexa Fluor® 680

sc-393357 AF680
200 µg/ml
$357.00

ARK-2 抗体 (A-3) Alexa Fluor® 790

sc-393357 AF790
200 µg/ml
$357.00

ARK-2 (A-3) 中和勝肽

sc-393357 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-393357 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
  • y_2025, m_8, d_27, h_5CST
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Rated 5 out of 5 by from Work well!1:200 diluted for wb of rat heart protein,the band is clear and the position is about 35kd
Date published: 2018-01-31
Rated 5 out of 5 by from Very goodGreat antibody with clear band for detecting ARK-2 in hepG2 cells
Date published: 2017-11-05
Rated 4 out of 5 by from Works well in WBThis antibody works well in WB. The dilution used was 1:3000. It gives two bands the first around 30 kDa and the second around 40 kDa.
Date published: 2017-10-17
Rated 5 out of 5 by from Very good results of ARK-2The antibody detects one specific band at a dilution of 1:600 in HepG2 cells. Very good.
Date published: 2017-07-06
Rated 5 out of 5 by from WBRight size band at 35kd in human macrophage samples by WB, but no nuclear signal by IHC-P.
Date published: 2016-12-24
Rated 5 out of 5 by from Very good Western blot data of ARKVery good Western blot data of ARK-2 expression in K-562 whole cell lysate. -SCBT QC
Date published: 2013-08-19
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ARK-2 抗体 (A-3) is rated 4.8 out of 5 by 6.
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