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Aquaporin 7/AQP7 Antibody (D-12) - Western Blotting - Image 358570
AQP7 抗体 (D-12): sc-376407. Hep G2 (A) 和 MCF10A (B) 全细胞裂解液中 AQP7 表达的 Western 印迹分析.
Aquaporin 7/AQP7 Antibody (D-12) - Western Blotting - Image 358570
AQP7 抗体 (D-12): sc-376407. Hep G2 (A) 和 MCF10A (B) 全细胞裂解液中 AQP7 表达的 Western 印迹分析.
Aquaporin 7/AQP7 Antibody (D-12) - Western Blotting - Image 155531
AQP7 抗体 (D-12): sc-376407. 大鼠睾丸组织提取物中 AQP7 表达的 Western 印迹分析.
AQP7 抗体 (D-12): sc-376407. Hep G2 (A) 和 MCF10A (B) 全细胞裂解液中 AQP7 表达的 Western 印迹分析.

Aquaporin 7/AQP7 抗体 (D-12): sc-376407

4.5(2)
寫評論提問

说明书
  • Aquaporin 7/AQP7 抗体 D-12 是小鼠单克隆 IgG2a κ，Aquaporin 7/AQP7抗体, 在12篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列169-269位于rat物种的AQP7的C-terminus
  • Aquaporin 7/AQP7 抗体 (D-12) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 AQP7
  • 抗Aquaporin 7/AQP7抗体（D-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Aquaporin 7/AQP7 (D-12): sc-376407的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是Aquaporin 7/AQP7 Antibody (D-12) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与Aquaporin 7/AQP7 Antibody (D-12) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外AQP抗体，包括AQP0, AQP1, AQP2, AQP3, AQP4, AQP5, AQP7, AQP8和AQP9

AQP7抗体（D-12）是一种IgG2a κ小鼠单克隆AQP7抗体（也称为水通道蛋白7抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的AQP7蛋白。AQP7抗体（D-12）既可作为非偶联形式的抗AQP7抗体提供，也可作为多种偶联形式的抗AQP7抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。水通道蛋白（AQPs）是一大类整体膜水通道蛋白，可促进水通过细胞膜的运输。该功能在动物、植物和细菌中是保守的。在哺乳动物中已经鉴定出许多水通道蛋白的同工型，分别命名为AQP0至AQP10。水通道蛋白分布广泛，同一细胞中存在多种AQP的情况并不少见。虽然大多数水通道蛋白仅对水具有渗透性，但AQP3、AQP7、AQP9和两种AQP10转录本中的一种也对尿素和甘油具有渗透性。水通道蛋白参与肾水吸收、肺部分泌物的产生、泪液分泌以及脑脊液和房水的分泌与再吸收。人类AQP7是一种由342个氨基酸组成的蛋白质，主要促进水、甘油和尿素的运输，主要在脂肪组织中表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Aquaporin 7/AQP7 抗体 (D-12) 参考文献:

  1. 哺乳动物水通道蛋白家族：有希望的新药靶点  |  Beitz, E. and Schultz, JE. 1999. Curr Med Chem. 6: 457-67. PMID: 10213793
  2. 利用 RT-PCR、免疫印迹法和免疫细胞化学法确定大鼠和小鼠肾脏中的 aquaporin-7 的位置。  |  Nejsum, LN., et al. 2000. Biochem Biophys Res Commun. 277: 164-70. PMID: 11027658
  3. 正常骨骼肌纤维中水蒸素 7 的表达和定位  |  Wakayama, Y., et al. 2004. Cell Tissue Res. 316: 123-9. PMID: 14986101
  4. 大鼠胃肠道中aquaporin-7的表达和免疫定位  |  Laforenza, U., et al. 2005. Biol Cell. 97: 605-13. PMID: 15943587
  5. 人眼中的水汽蛋白 6-12  |  Tran, TL., et al. 2013. Acta Ophthalmol. 91: 557-63. PMID: 22974000
  6. 青光眼眼球中的水蒸发蛋白表达发生改变。  |  Tran, TL., et al. 2014. APMIS. 122: 772-80. PMID: 24479869
  7. 人与兔声带褶皱中整体膜蛋白定位的比较  |  Gartling, GJ., et al. 2021. Laryngoscope. 131: E1265-E1271. PMID: 33155693
  8. 人和小鼠白色脂肪组织中水甘油多肽的定位。  |  Iena, FM., et al. 2022. Histochem Cell Biol. 157: 623-639. PMID: 35235046
  9. 一种仅在人类肾脏中表达的新型水通道的 cDNA 克隆和基因结构：染色体 12q13 位点水蒸发蛋白基因簇的证据。  |  Ma, T., et al. 1996. Genomics. 35: 543-50. PMID: 8812490
  10. 具有甘油渗透性的脂肪组织新型人水蒸素的分子克隆和表达。  |  Kuriyama, H., et al. 1997. Biochem Biophys Res Commun. 241: 53-8. PMID: 9405233
  11. 人类 Aquaporin-7 基因的分子特征及其染色体图谱。  |  Ishibashi, K., et al. 1998. Biochim Biophys Acta. 1399: 62-6. PMID: 9714739
  12. 水通道蛋白。  |  Echevarría, M. and Ilundáin, AA. 1998. J Physiol Biochem. 54: 107-18. PMID: 9858131

订购信息

产品名称产品编号规格价格数量收藏夹

Aquaporin 7/AQP7 抗体 (D-12)

sc-376407
200 µg/ml
$316.00

Aquaporin 7/AQP7 (D-12): m-IgGκ BP-HRP 套装

sc-522946
200 µg Ab, 40 µg BP
$354.00

Aquaporin 7/AQP7 抗体 (D-12) AC

sc-376407 AC
500 µg/ml, 25% agarose
$416.00

Aquaporin 7/AQP7 抗体 (D-12) HRP

sc-376407 HRP
200 µg/ml
$316.00

Aquaporin 7/AQP7 抗体 (D-12) FITC

sc-376407 FITC
200 µg/ml
$330.00

Aquaporin 7/AQP7 抗体 (D-12) PE

sc-376407 PE
200 µg/ml
$343.00

Aquaporin 7/AQP7 抗体 (D-12) Alexa Fluor® 488

sc-376407 AF488
200 µg/ml
$357.00

Aquaporin 7/AQP7 抗体 (D-12) Alexa Fluor® 546

sc-376407 AF546
200 µg/ml
$357.00

Aquaporin 7/AQP7 抗体 (D-12) Alexa Fluor® 594

sc-376407 AF594
200 µg/ml
$357.00

Aquaporin 7/AQP7 抗体 (D-12) Alexa Fluor® 647

sc-376407 AF647
200 µg/ml
$357.00

Aquaporin 7/AQP7 抗体 (D-12) Alexa Fluor® 680

sc-376407 AF680
200 µg/ml
$357.00

Aquaporin 7/AQP7 抗体 (D-12) Alexa Fluor® 790

sc-376407 AF790
200 µg/ml
$357.00

Hi Do you know if this antibody is functional in IF or IHC on human kidney tissues? has anyone tried it? Regards

Asked by: jado
Thank you for you question. sc-376407 is just recommended for detection of AQP7 of mouse, rat and human origin by WB, IP, IF and ELISA.
Answered by: Tech Support Europe
Date published: 2024-01-29

For anyone who has used this ab in blotting against AQP-7 in brown adipose tissue (mouse): did you detect specific bands at 75-80 kDa or at any other unpredicted MW?

Asked by: mina
Thank you for your question. We do not have any data for 75-80 kDa. To discuss your blot please contact technical service by email at scbt@scbt.com or call (800)-457-3801.
Answered by: BlakeJ
Date published: 2023-12-01

Could this antibody be tried with Flow Cytometery for bovine aquaporin?

Asked by: KaleemSarwar
Thank you for your question. This antibody does not have any data or product citations in bovine samples. This monoclonal antibody is raised against amino acids 169-269 mapping at the C-terminus of AQP7 of rat origin. This antigenic sequence only shares approximately 62% sequence identity with AQP7 in bovine (accession number NP_001069846). We do not know the exact epitope recognized by this monoclonal antibody. But with only 62% sequence identity between the antigen and the bovine protein, we would not expect this antibody to cross-react with bovine. That would have to be determined experimentally. We are aware of one product citation using this antibody for flow cytometry in mouse samples. Please refer to PMID: 31990671 for details.
Answered by: Technical Support
Date published: 2020-05-14

Where is the epitope of the antibody? Is the staining extracellular, cytosolic or plasmatic membrane? Can you pass me references please?

Asked by: lspoblet
Thank you for your question. This mouse monoclonal antibody is raised against amino acids 169-269 mapping at the C-terminus of AQP7 of rat origin. The exact epitope recognized by this clone has not been determined, and since this antigen includes both extracellular and cytoplasmic domains it could recognize either of these subcellular locations. We are not aware of any product citations yet for this newer antibody.
Answered by: Technical Support
Date published: 2018-11-20

What is the recommended starting dilution for Western blot for sc-376407: AQP7 (D-12) monoclonal antibody?

Asked by: AbPolly
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from Anti-aquaglyceroporin 7I did not have problems in the westernblot using this antibody
Date published: 2018-10-19
Rated 5 out of 5 by from Worked well in WB in rat testis tissue extractWorked well in WB in rat testis tissue extract. -SCBT QC
Date published: 2013-08-19
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Aquaporin 7/AQP7 抗体 (D-12) is rated 4.5 out of 5 by 2.
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