Amino acid ABC transporter permease/substrate-binding protein (E-5): sc-393959. Western blot analysis of Amino acid ABC transporter permease/substrate-binding protein expression in Streptococcus equi (virulent) (A), Streptococcus equi (avirulent) (B), Rhodococcus equi (C) and Escherichia coli (D) whole cell lysates. Note lack of reactivity with unrelated bacterial lysates in lanes C and D.
Amino acid-binding ABC transporter Antibody (E-5): sc-393959
- Amino acid-binding ABC transporter Antibody (E-5) is a mouse monoclonal IgG2a κ Amino acid-binding ABC transporter antibody provided at 200 µg/ml
- raised against amino acids 1-278 representing full length Amino acid-binding ABC transporter protein of Streptococcus equi subsp. equi 4047 origin
- Amino acid-binding ABC transporter Antibody (E-5) is recommended for detection of Amino acid-binding ABC transporter protein of S. equi origin by WB, IP, IF and ELISA
- Anti-Amino acid-binding ABC transporter Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP is the preferred secondary detection reagent for Amino acid-binding ABC transporter Antibody (E-5) for WB applications. This reagent is now offered in a bundle with Amino acid-binding ABC transporter Antibody (E-5) (see ordering information below).
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Amino acid-binding ABC transporter Antibody (E-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects Amino acid-binding ABC transporter of S. equi origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Amino acid-binding ABC transporter antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Amino acid-binding ABC transporter plays a crucial role in transporting amino acids across cellular membranes, maintaining cellular homeostasis and supporting various metabolic processes. Amino acid-binding ABC transporter is primarily located in plasma membranes, facilitating essential amino acid uptake necessary for protein synthesis and metabolic functions. Amino acid-binding ABC transporter enables S. equi to acquire nutrients needed for growth and virulence, contributing to strangles pathogenesis - a highly contagious disease characterized by severe respiratory symptoms and lymphadenitis. S. equi infections spread through inhalation or direct contact with infected materials, making Amino acid-binding ABC transporter function and location understanding critical for developing effective therapeutic strategies.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Amino acid-binding ABC transporter Antibody (E-5) References:
- Functional characterization of monocarboxylic acid, large neutral amino acid, bile acid and peptide transporters, and P-glycoprotein in MDCK and Caco-2 cells. | Putnam, WS., et al. 2002. J Pharm Sci. 91: 2622-35. PMID: 12434407
- Functional assignment of solute-binding proteins of ABC transporters using a fluorescence-based thermal shift assay. | Giuliani, SE., et al. 2008. Biochemistry. 47: 13974-84. PMID: 19063603
- Getting to grips with strangles: an effective multi-component recombinant vaccine for the protection of horses from Streptococcus equi infection. | Guss, B., et al. 2009. PLoS Pathog. 5: e1000584. PMID: 19763180
- Molecular characterisation of 'strangles' outbreaks in the UK: the use of M-protein typing of Streptococcus equi ssp. equi. | Ivens, PA., et al. 2011. Equine Vet J. 43: 359-64. PMID: 21492215
- Association of Streptococcus equi with equine monocytes. | Mérant, C., et al. 2011. Vet Immunol Immunopathol. 143: 83-6. PMID: 21752476
- Streptococcus equi: a pathogen restricted to one host. | Waller, AS., et al. 2011. J Med Microbiol. 60: 1231-1240. PMID: 21757503
- Streptococcus equi subspecies equi infection (strangles) in horses. | Boyle, A. 2011. Compend Contin Educ Vet. 33: E1-7; quiz E8. PMID: 21870348
- Antiphagocytic function of an IgG glycosyl hydrolase from Streptococcus equi subsp. equi and its use as a vaccine component. | Flock, M., et al. 2012. Infect Immun. 80: 2914-9. PMID: 22615244
- Development of a novel mucosal vaccine against strangles by supercritical enhanced atomization spray-drying of Streptococcus equi extracts and evaluation in a mouse model. | Rodrigues, MA., et al. 2012. Eur J Pharm Biopharm. 82: 392-400. PMID: 22841882
- Detection of Streptococcus equi subspecies equi using a triplex qPCR assay. | Webb, K., et al. 2013. Vet J. 195: 300-4. PMID: 22884566
- Biophysical insights into a highly selective l-arginine-binding lipoprotein of a pathogenic treponeme. | Deka, RK., et al. 2018. Protein Sci. 27: 2037-2050. PMID: 30242931
- Absence of basal ganglia amino acid neuron deficits in schizophrenia in three collections of brains. | Simpson, MD., et al. 1998. Schizophr Res. 31: 167-75. PMID: 9689721
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Amino acid-binding ABC transporter Antibody (E-5) | sc-393959 | 200 µg/ml | $322.00 | |||
Amino acid-binding ABC transporter Antibody (E-5): m-IgG2a BP-HRP Bundle | sc-545969 | 200 µg Ab; 10 µg BP | $361.00 | |||
Amino acid-binding ABC transporter Antibody (E-5) AC | sc-393959 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Amino acid-binding ABC transporter Antibody (E-5) HRP | sc-393959 HRP | 200 µg/ml | $322.00 | |||
Amino acid-binding ABC transporter Antibody (E-5) FITC | sc-393959 FITC | 200 µg/ml | $336.00 | |||
Amino acid-binding ABC transporter Antibody (E-5) PE | sc-393959 PE | 200 µg/ml | $349.00 | |||
Amino acid-binding ABC transporter Antibody (E-5) Alexa Fluor® 488 | sc-393959 AF488 | 200 µg/ml | $364.00 | |||
Amino acid-binding ABC transporter Antibody (E-5) Alexa Fluor® 546 | sc-393959 AF546 | 200 µg/ml | $364.00 | |||
Amino acid-binding ABC transporter Antibody (E-5) Alexa Fluor® 594 | sc-393959 AF594 | 200 µg/ml | $364.00 | |||
Amino acid-binding ABC transporter Antibody (E-5) Alexa Fluor® 647 | sc-393959 AF647 | 200 µg/ml | $364.00 | |||
Amino acid-binding ABC transporter Antibody (E-5) Alexa Fluor® 680 | sc-393959 AF680 | 200 µg/ml | $364.00 | |||
Amino acid-binding ABC transporter Antibody (E-5) Alexa Fluor® 790 | sc-393959 AF790 | 200 µg/ml | $364.00 |