Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
alpha 1 Sodium Potassium ATPase/ATP1A1 Double Nickase Plasmid (h): sc-400239-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- alpha 1 Sodium Potassium ATPase/ATP1A1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- alpha 1 Sodium Potassium ATPase/ATP1A1 Double Nickase Plasmid (h) and alpha 1 Sodium Potassium ATPase/ATP1A1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ATP1A1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (C464.6): sc-21712
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
alpha 1 Sodium Potassium ATPase/ATP1A1 Double Nickase Plasmid (h) | sc-400239-NIC | 20 µg | $410.00 | |||
alpha 1 Sodium Potassium ATPase/ATP1A1 Double Nickase Plasmid (h2) | sc-400239-NIC-2 | 20 µg | $410.00 |
ATP1A1 encodes the catalytic α1 subunit of the Na⁺/K⁺-ATPase, an essential P-type ATPase that uses ATP hydrolysis to export Na⁺ and import K⁺ across the plasma membrane. By maintaining ionic gradients, ATP1A1 supports resting membrane potential, cell volume control, secondary active transport, and excitability, and it influences signaling pathways linked to adhesion, cytoskeletal organization, and stress responses. α1 Na⁺/K⁺-ATPase activity is central to epithelial and neuronal physiology and contributes to ion homeostasis programs that shape metabolic demand and Ca²⁺ handling. Dysregulated ATP1A1 function or expression has been associated with altered excitability and transport phenotypes in disease-relevant contexts, making it a useful node for studying membrane physiology and signaling cross-talk.
alpha 1 Sodium Potassium ATPase/ATP1A1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ATP1A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ATP1A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ATP1A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ATP1A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.