Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (C464.6) - Western Blotting - Image 4626
Na+/K+- ATPase α1 (C464. 6): sc-21712. HeLa (A) 和 MDCK (B) 全细胞裂解液及大鼠胎盘组织提取物 (C) 中 Na+/K+- ATPase α1 表达的 Western 印迹分析.
Na+/K+- ATPase α1 (C464. 6): sc-21712. HeLa (A) 和 MDCK (B) 全细胞裂解液及大鼠胎盘组织提取物 (C) 中 Na+/K+- ATPase α1 表达的 Western 印迹分析.

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (C464.6): sc-21712

4.8(21)
寫評論提問

说明书
  • alpha 1 Sodium Potassium ATPase/ATP1A1抗体(C464.6)是小鼠单克隆IgG1 κ, 在206篇文献中引用，规格为200 µg/ml
  • 纯化的rabbit肾外髓质
  • alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (C464.6) 推荐用于WB, IP, IF 和 IHC(P)，检测broad species 来源的 Na+/K+-ATPase α1
  • 抗alpha 1 Sodium Potassium ATPase/ATP1A1抗体（C464.6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取alpha 1 Sodium Potassium ATPase/ATP1A1 (C464.6): sc-21712的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (C464.6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (C464.6) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
说明书与实验方案
研究信息

関連項目

额外Sodium/Potassium-ATPase Protein抗体，包括H+/K+ ATPase β, Na+/K+-ATPase α, Na+/K+-ATPase β 1, Na+/K+-ATPase β 3和Na+/K+-ATPase α 1

Na+/K+-ATPase α1 抗体（C464.6）是一种小鼠单克隆 IgG1 kappa 轻链抗体，经过工程改造，可识别多种物种的 Na+/K+-ATPase α1 (ATP1A1)，适用于多种应用，如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）。Na+/K+-ATPase α1 单克隆抗体（C464.6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 结合型，可满足不同的研究需求。Na+/K+-ATP酶是P型离子转运蛋白家族中一种重要的膜蛋白，通过主动将三个钠离子泵出细胞，将两个钾离子泵入细胞，从而维持细胞内环境的稳定。这一作用可调节细胞体积，维持静止膜电位，并实现各种溶质的二次主动转运。Na+/K+-ATP酶复合物由α、β和γ亚基的多个同工型组成；α1亚基包含ATP和离子结合位点，而β亚基则确保α亚基的正确折叠和膜插入。γ亚基可增强特定组织（尤其是肾脏的肾小节）中ATP的亲和力。Na+/K+-ATPase α1 (C464.6) 抗体使研究人员能够研究这种重要蛋白在各种生物学环境中的调节和作用，从而增进对细胞机制、高血压、心脏功能和神经疾病的理解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (C464.6) 参考文献:

  1. Na,K-ATPase gamma 亚基的 gamma a 和 gamma b 形式的功能作用和免疫细胞化学定位。  |  Pu, HX., et al. 2001. J Biol Chem. 276: 20370-8. PMID: 11278761
  2. 化学修饰是阐明钠泵结构与功能关系的一种方法。  |  Pedemonte, CH. and Kaplan, JH. 1990. Am J Physiol. 258: C1-23. PMID: 2154108
  3. 钠泵需要其 beta 亚基。  |  McDonough, AA., et al. 1990. FASEB J. 4: 1598-605. PMID: 2156741
  4. Na,K-ATPaseα-亚基和β-亚基在正确的翻译后处理和细胞内转运方面相互依赖。  |  Ackermann, U. and Geering, K. 1990. FEBS Lett. 269: 105-8. PMID: 2167238
  5. 一种与 Na,K-ATP 酶相关的蛋白脂质对 ATP 酶的活性并非必不可少。  |  Hardwicke, PM. and Freytag, JW. 1981. Biochem Biophys Res Commun. 102: 250-7. PMID: 6272791
  6. 异源二聚体 P 型 ATP 酶 beta 亚基的功能意义。  |  Chow, DC. and Forte, JG. 1995. J Exp Biol. 198: 1-17. PMID: 7891030
  7. Na+,K+ -ATP酶。  |  Lingrel, JB. and Kuntzweiler, T. 1994. J Biol Chem. 269: 19659-62. PMID: 8051040
  8. 在酵母细胞中表达功能性钠泵的亚基要求。  |  Scheiner-Bobis, G. and Farley, RA. 1994. Biochim Biophys Acta. 1193: 226-34. PMID: 8054343
  9. Na,K-ATP 酶的α-亚基具有独立于β-亚基的催化活性。  |  Blanco, G., et al. 1994. J Biol Chem. 269: 23420-5. PMID: 8089106
  10. 在杆状病毒感染的昆虫细胞中表达, 定位和组装功能性 Na,K-ATP 酶多肽。  |  DeTomaso, AW., et al. 1993. J Biol Chem. 268: 1470-8. PMID: 8380413
  11. 与 Na,K-ATP 酶相关的小蛋白--γ 多肽的分子克隆和免疫学特征。  |  Mercer, RW., et al. 1993. J Cell Biol. 121: 579-86. PMID: 8387529
  12. Na,K 泵的结构和功能：分子生物学十年。  |  Pressley, TA. 1996. Miner Electrolyte Metab. 22: 264-71. PMID: 8933497

订购信息

产品名称产品编号规格价格数量收藏夹

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (C464.6)

sc-21712
200 µg/ml
$316.00

alpha 1 Sodium Potassium ATPase/ATP1A1 (C464.6): m-IgGκ BP-HRP 套装

sc-520721
200 µg Ab, 40 µg BP
$354.00

alpha 1 Sodium Potassium ATPase/ATP1A1 (C464.6): m-IgG1 BP-HRP 套装

sc-533469
200 µg Ab; 20 µg BP
$354.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (C464.6) AC

sc-21712 AC
500 µg/ml, 25% agarose
$416.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (C464.6) HRP

sc-21712 HRP
200 µg/ml
$316.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (C464.6) FITC

sc-21712 FITC
200 µg/ml
$330.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (C464.6) PE

sc-21712 PE
200 µg/ml
$343.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (C464.6) Alexa Fluor® 488

sc-21712 AF488
200 µg/ml
$357.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (C464.6) Alexa Fluor® 546

sc-21712 AF546
200 µg/ml
$357.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (C464.6) Alexa Fluor® 594

sc-21712 AF594
200 µg/ml
$357.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (C464.6) Alexa Fluor® 647

sc-21712 AF647
200 µg/ml
$357.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (C464.6) Alexa Fluor® 680

sc-21712 AF680
200 µg/ml
$357.00

alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (C464.6) Alexa Fluor® 790

sc-21712 AF790
200 µg/ml
$357.00

If this antibody can test for mouse samples?

Asked by: Yanina
Thank you for your question. Yes, this antibody is fine for mouse samples.
Answered by: Tech Service
Date published: 2019-11-01

What is the epitope sequence recognized by Na+/K+ Atpase alpha1 antibody? is the epitope recognized by Na+/K+ Atpase alpha1 antibody (C464.6) conserved in Human?

Asked by: Lupe
Thank you for your question. This antibody was raised against purified rabbit renal outer medulla. The exact binding epitope has not been mapped. If you have any further questions, please contact Technical Service.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

Does this antibody react with xenopus tissue ? Thank you.

Asked by: KKKKKKKKK
Thank you for your question. This antibody has not been tested on Xenopus tissue, so we do not have information to support that use. However, it is broadly reactive and may very well react with Xenopus samples. Please contact Technical Service by phone, email or by live chat directly on our website, www.scbt.com, if you would like to try a sample of this antibody.
Answered by: Tech Service
Date published: 2017-10-26

What is the epitope recognized by Na+/K+-ATPase α1 Antibody (C464.6)?

Asked by: cjMara
Thank you for your question. Na+/K+-ATPase α1 Antibody (C464.6) is a mouse monoclonal IgG1 raised against purified rabbit renal outer medulla. The exact epitope recognized by this antibody has not been determined.
Answered by: Technical Support
Date published: 2016-12-22

I need to use Na+/K+-ATPase α1 Antibody (C464.6): sc-21712 for Western blot in canine samples. Does this antibody react with canine?

Asked by: cjMara
Thank you for your question. Yes, this antibody has been cited in a few publications for use in canine samples. For reference please see, PMID: 16701566, 16701566 & 18778408 on PubMed.
Answered by: Technical Support
Date published: 2016-12-22
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_5
  • loc_zh_CN, sid_21712, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 99ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from WORKS WELL IN WBApplcation WB Cells: CFBE (human bronchial cell line) Dilution: 1 :1000 Very specific band. Not strong signal but clean and with no background. We used the antibody as loading control.
Date published: 2025-03-20
Rated 3 out of 5 by from Na+/K+-ATPase α1Good western blots with sc-21712 antibody obtained on rat heart lysate samples.
Date published: 2020-10-20
Rated 5 out of 5 by from Worked greatGood for immunofluorescence on paraffin-embedded mouse tissue
Date published: 2020-10-06
Rated 5 out of 5 by from Great featuresWorked great for immunochemistry for murine samples.
Date published: 2019-11-18
Rated 5 out of 5 by from Worked greatWorked great at 1:100 for IF for human tissues. Very good.
Date published: 2019-09-25
Rated 5 out of 5 by from Na+,K+-ATP ase alpha 1 subunit renal tissueWe used this antibody in renal cortex and medulla. We performed a western blot and immunohistochemistry. We could visualized a very good band at 100kDa in western blot. Immunohistochemistry showed a prominent Na+,K+-ATP ase alpha1 in basolateral membrane from renal tubules.
Date published: 2019-02-08
Rated 5 out of 5 by from Very good antibodyThis antibody gave very clean membrane staining in cells by immunofluorescence.
Date published: 2018-04-30
Rated 5 out of 5 by from great featuresI bought this antibody for a while, and I'd used it for my experiments for a long time, I think I'm capable for writting down the review for this antibody. This antibody was very great, the sigals of Na/K-ATPase was very clear, and, of course, the PVDF membrane's background was totally clean. This antibody is worth to be used.
Date published: 2017-12-22
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_21
  • loc_zh_CN, sid_21712, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 10ms
  • REVIEWS, PRODUCT
alpha 1 Sodium Potassium ATPase/ATP1A1 抗体 (C464.6) is rated 4.8 out of 5 by 21.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_21
  • loc_zh_CN, sid_21712, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 106ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。