Date published: 2025-9-8

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Alkaline Phosphatase 抗体 (A-10): sc-271431

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说明书
  • Alkaline Phosphatase 抗体 A-10 是小鼠单克隆 IgG2a κ,Alkaline Phosphatase抗体, 在44篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的PLAP的内部区域内的氨基酸66-111之间
  • Alkaline Phosphatase 抗体 (A-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测 PLAP, ALPPL2 and IAP of human origin, and Akp-3 of mouse and rat origin
  • 抗Alkaline Phosphatase抗体(A-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Alkaline Phosphatase (A-10): sc-271431免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2a BP-HRP 是Alkaline Phosphatase 抗体 (A-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Alkaline Phosphatase 抗体 (A-10) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

碱性磷酸酶抗体(A-10)是一种IgG2a κ小鼠单克隆碱性磷酸酶抗体,可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人的碱性磷酸酶蛋白。碱性磷酸酶抗体(A-10)既可以是非偶联的抗碱性磷酸酶抗体形式,也可以是多种偶联形式的抗碱性磷酸酶抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。碱性磷酸酶(AP)是糖基-磷脂酰肌醇(GPI)锚定的、二聚体的、Zn2+金属化的糖蛋白,可催化磷酸单酯水解为无机磷酸和醇。胎盘碱性磷酸酶(也称为PLAP、ALPP、PALP、胎盘ALP-1或Regan同工酶)是一种由530个氨基酸组成的组织特异性AP,在胎盘、孕妇血清中表达,并在各种癌症中异位表达,包括卵巢和睾丸癌症。PLAP可能有助于引导迁移细胞和运输特定分子(如脂肪酸和免疫球蛋白)穿过细胞膜。在人类中已发现的三种组织特异性AP,即PLAP、生殖细胞AP(GCAP)和肠AP,具有90-98%的同源性,且它们的基因聚集在2号染色体2q上。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Alkaline Phosphatase 抗体 (A-10) 参考文献:

  1. 人类恶性肿瘤中胎盘碱性磷酸酶的表达概况:肿瘤细胞活化对碱性磷酸酶表达的影响。  |  Dabare, AA., et al. 1999. Urol Int. 63: 168-74. PMID: 10738188
  2. 分辨率为 1.8 A 的人类胎盘碱性磷酸酶晶体结构。底物特异性的含义。  |  Le Du, MH., et al. 2001. J Biol Chem. 276: 9158-65. PMID: 11124260
  3. 子宫内膜异位症妇女腹腔液中的胎盘型碱性磷酸(酯)酶。  |  Kang, JO., et al. 1990. Clin Chim Acta. 186: 285-94. PMID: 2311256
  4. 人胃癌细胞产生的胎盘碱性磷酸(酯)酶同工酶  |  Aizawa, K., et al. 1989. Acta Pathol Jpn. 39: 630-7. PMID: 2686348
  5. 血清胎盘样碱性磷酸酶(PLAP):用于监测卵巢癌的新型酶联免疫测定。  |  Fisken, J., et al. 1989. J Clin Pathol. 42: 40-5. PMID: 2921344
  6. 通过 H17E2 单克隆抗体测定评估睾丸生殖细胞肿瘤中胎盘碱性磷酸酶样活性的血清标记物潜力。  |  Tucker, DF., et al. 1985. Br J Cancer. 51: 631-9. PMID: 2986664
  7. 碱性磷酸(酯)酶及其同工酶的诊断方面。  |  Moss, DW. 1987. Clin Biochem. 20: 225-30. PMID: 3319285
  8. 人类胎盘和肠道碱性磷酸(酯)酶基因位于2q34-q37。  |  Griffin, CA., et al. 1987. Am J Hum Genet. 41: 1025-34. PMID: 3687940
  9. 监测睾丸肿瘤的血清胎盘碱性磷酸酶单克隆抗体测定。  |  Epenetos, AA., et al. 1985. Br J Cancer. 51: 641-4. PMID: 3994908
  10. 针对胎盘碱性磷酸酶和其他人类滋养层相关决定因子的单克隆抗体的制备和表征。  |  Travers, P. and Bodmer, W. 1984. Int J Cancer. 33: 633-41. PMID: 6202646
  11. 使用两种不同的胎盘碱性磷酸酶单克隆抗体对睾丸生殖细胞瘤进行免疫组织学研究。  |  Epenetos, AA., et al. 1984. Br J Cancer. 49: 11-5. PMID: 6362705
  12. 人类碱性磷酸(酯)酶生物学,特别是癌症。  |  Millán, JL. and Fishman, WH. 1995. Crit Rev Clin Lab Sci. 32: 1-39. PMID: 7748466

订购信息

产品名称产品编号规格价格数量收藏夹

Alkaline Phosphatase 抗体 (A-10)

sc-271431
200 µg/ml
$316.00

Alkaline Phosphatase (A-10): m-IgG2a BP-HRP 套装

sc-551925
200 µg Ab; 10 µg BP
$354.00

Alkaline Phosphatase 抗体 (A-10) AC

sc-271431 AC
500 µg/ml, 25% agarose
$416.00

Alkaline Phosphatase 抗体 (A-10) HRP

sc-271431 HRP
200 µg/ml
$316.00

Alkaline Phosphatase 抗体 (A-10) FITC

sc-271431 FITC
200 µg/ml
$330.00

Alkaline Phosphatase 抗体 (A-10) PE

sc-271431 PE
200 µg/ml
$343.00

Alkaline Phosphatase 抗体 (A-10) Alexa Fluor® 488

sc-271431 AF488
200 µg/ml
$357.00

Alkaline Phosphatase 抗体 (A-10) Alexa Fluor® 546

sc-271431 AF546
200 µg/ml
$357.00

Alkaline Phosphatase 抗体 (A-10) Alexa Fluor® 594

sc-271431 AF594
200 µg/ml
$357.00

Alkaline Phosphatase 抗体 (A-10) Alexa Fluor® 647

sc-271431 AF647
200 µg/ml
$357.00

Alkaline Phosphatase 抗体 (A-10) Alexa Fluor® 680

sc-271431 AF680
200 µg/ml
$357.00

Alkaline Phosphatase 抗体 (A-10) Alexa Fluor® 790

sc-271431 AF790
200 µg/ml
$357.00

Alkaline Phosphatase (A-10) 中和勝肽

sc-271431 P
100 µg/0.5 ml
$68.00

this antibody can be use in rat?

Asked by: tx3359836
Thank you for your question. Yes, this mouse monoclonal antibody, Alkaline Phosphatase (A-10): sc-271431, can be used in rat. We are aware of two publications citing use of the antibody in rat samples. These include PMID numbers: 25662160 and 26781793.
Answered by: Technical Support
Date published: 2018-11-11

What application is the blocking peptide sc-271431 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2024-07-23
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Rated 5 out of 5 by from Used in Mesenchymal Stem CellsThis antibody worked well with MSCs, used 1:500 dilution.
Date published: 2019-09-12
Rated 3 out of 5 by from Does not detect SEAP in western blotI tried to detect exogenously expressed SEAP (secreted alkaline phosphatase - a truncated version of PLAP) in HeLa cell lysate and media supernatant with this antibody in western blot (1:200 dilution). It didn't detect SEAP either in media or in cell lysate, even if the immunogen of this antibody should also be present in SEAP.
Date published: 2018-02-23
Rated 1 out of 5 by from A Blur of False Hope and Empty PromisesUsed a 1:250 dilution attempting to probe human osteosarcoma cells. The result was a faint blur at approximately the correct size, but by no means a well-define band. Attempted to incubate primary antibody again at 4 degrees overnight, and the band disappeared. No concentration of primary or secondary antibodies could resurrect it.
Date published: 2018-01-05
Rated 5 out of 5 by from IHC/PWork well in the IHC/P of mouse tibia(1:100). Osteoblasts and other cells could be stained.
Date published: 2017-10-23
Rated 5 out of 5 by from Great Western blot data of PLAP expression in nonGreat Western blot data of PLAP expression in non-transfected and human PLAP transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-12-01
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Alkaline Phosphatase 抗体 (A-10) is rated 3.8 out of 5 by 5.
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