Date published: 2025-9-7
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ALK Antibody (F-12) - Western Blotting - Image 308430
ALK 抗体 (F-12): sc-398791. HuT 78 (A), U-937 (B), Raji (C), K-562 (D), MDA-MB-231 (E) 和 MDA-MB-435S (F) 全细胞裂解液中 ALK 表达的 Western 印迹分析.
ALK 抗体 (F-12): sc-398791. HuT 78 (A), U-937 (B), Raji (C), K-562 (D), MDA-MB-231 (E) 和 MDA-MB-435S (F) 全细胞裂解液中 ALK 表达的 Western 印迹分析.

ALK 抗体 (F-12): sc-398791

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说明书
  • ALK 抗体 F-12 是小鼠单克隆 IgG2a κ，ALK抗体, 在13篇文献中引用，规格为200 µg/ml
  • 特定于human起源的ALK的N-terminal胞外结构域内氨基酸117-145之间的表位图谱
  • ALK 抗体 (F-12) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 ALK
  • 抗ALK抗体（F-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ALK (F-12): sc-398791的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是ALK Antibody (F-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与ALK Antibody (F-12) 打包提供（请参阅下面的订购信息）。

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描述
说明书与实验方案
研究信息

関連項目

ALK抗体（F-12）是一种IgG2a κ小鼠单克隆ALK抗体（也称为CD246抗体、ALK RTK抗体或1型受体酪氨酸激酶ALK抗体），它可以通过蛋白质印迹法（WB）、免疫沉淀法（IP）、免疫荧光法（IF）和酶联免疫吸附测定法（ELISA）检测小鼠、大鼠和人类来源的ALK蛋白。ALK抗体（F-12）既可以是非偶联的抗ALK抗体形式，也可以是多种偶联形式的抗ALK抗体，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。LTK、ALK和Ros已被鉴定为受体酪氨酸激酶，它们与胰岛素受体亚家族激酶具有序列相似性。LTK（白细胞酪氨酸激酶）在小鼠B淋巴细胞前体中表达，也存在于前脑神经元中。ALK（间变性淋巴瘤激酶）通常在神经系统中高表达。在许多非霍奇金淋巴瘤中，由于发生了易位，会表达一种包含ALK催化结构域的截短形式。c-Ros基因最初是作为癌基因的突变形式被鉴定出来的。Ros通常在少数上皮细胞类型中表达，可能在上皮发育中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ALK 抗体 (F-12) 参考文献:

  1. 神经元中交替剪接的 ltk mRNA 预测受体具有较大的假定胞外结构域。  |  Haase, VH., et al. 1991. Oncogene. 6: 2319-25. PMID: 1662793
  2. 人类胶质母细胞瘤细胞系中 ROS1 cDNA 的特征。  |  Birchmeier, C., et al. 1990. Proc Natl Acad Sci U S A. 87: 4799-803. PMID: 2352949
  3. 克隆小鼠 c-ros 肾 cDNA, 其在发育中的作用以及与细胞外基质糖蛋白的关系。  |  Kanwar, YS., et al. 1995. Kidney Int. 48: 1646-59. PMID: 8544427
  4. c-ros 酪氨酸激酶受体控制着附睾上皮细胞的区域化和分化。  |  Sonnenberg-Riethmacher, E., et al. 1996. Genes Dev. 10: 1184-93. PMID: 8675006
  5. 通过白细胞酪氨酸激酶（一种与胰岛素受体相关的酪氨酸激酶）中两个不同的 NPXY 矩阵传递生长和生存信号。  |  Ueno, H., et al. 1996. J Biol Chem. 271: 27707-14. PMID: 8910363
  6. ALK 的分子特征，ALK 是一种专门在神经系统中表达的受体酪氨酸激酶。  |  Iwahara, T., et al. 1997. Oncogene. 14: 439-49. PMID: 9053841

订购信息

产品名称产品编号规格价格数量收藏夹

ALK 抗体 (F-12)

sc-398791
200 µg/ml
$316.00

ALK (F-12): m-IgG Fc BP-HRP 套装

sc-530930
200 µg Ab; 10 µg BP
$354.00

ALK (F-12): m-IgGκ BP-HRP 套装

sc-524488
200 µg Ab, 40 µg BP
$354.00

ALK (F-12): m-IgG2a BP-HRP 套装

sc-547637
200 µg Ab; 10 µg BP
$354.00

ALK 抗体 (F-12) AC

sc-398791 AC
500 µg/ml, 25% agarose
$416.00

ALK 抗体 (F-12) HRP

sc-398791 HRP
200 µg/ml
$316.00

ALK 抗体 (F-12) FITC

sc-398791 FITC
200 µg/ml
$330.00

ALK 抗体 (F-12) PE

sc-398791 PE
200 µg/ml
$343.00

ALK 抗体 (F-12) Alexa Fluor® 488

sc-398791 AF488
200 µg/ml
$357.00

ALK 抗体 (F-12) Alexa Fluor® 546

sc-398791 AF546
200 µg/ml
$357.00

ALK 抗体 (F-12) Alexa Fluor® 594

sc-398791 AF594
200 µg/ml
$357.00

ALK 抗体 (F-12) Alexa Fluor® 647

sc-398791 AF647
200 µg/ml
$357.00

ALK 抗体 (F-12) Alexa Fluor® 680

sc-398791 AF680
200 µg/ml
$357.00

ALK 抗体 (F-12) Alexa Fluor® 790

sc-398791 AF790
200 µg/ml
$357.00

ALK (F-12) 中和勝肽

sc-398791 P
100 µg/0.5 ml
$68.00

What is the recommending dilution for ELISA?

Asked by: EH123
Than you for your question. When using ALK (F-12): sc-398791 in solid phase ELISA we recommend a starting dilution of 1:30, with a dilution range of 1:30-1:3000.
Answered by: Tech Support Europe
Date published: 2024-04-19

I want to know whether the antibody (ALK Antibody (F-12): sc-398791) conjugated HRP can be used to examine the ALK rearrangement. Thank you!

Asked by: Leo1234
Thank you for your question. The HRP conjugated antibody is recommended for for WB, IHC(P) and ELISA. It is unclear what your intended research application is that you refer to as examining the ALK rearrangment. Please contact our Technical Service Department for further support.
Answered by: Technical Support
Date published: 2018-12-06

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with ALK Antibody (F-12): sc-398791?

Asked by: Trav11
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-25
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Rated 4 out of 5 by from Great featuresI use this antibody on pancreatic cancer cell line and have good bands.
Date published: 2018-03-14
Rated 5 out of 5 by from Good signalI used it for WB at 1:500 dilution, specific and clean signal
Date published: 2017-10-24
Rated 5 out of 5 by from Great feature!I used it in WB assay at 1:1000 and it works very well
Date published: 2017-07-12
Rated 5 out of 5 by from excellent antibody for westernsprobed at 1/500X dilution for 90min and used in conjunction with the Kappa-binding-HRP as secondary and the blot turned out perfect. Excellent antibody for western blotting.
Date published: 2017-06-23
Rated 5 out of 5 by from Excellent for WBThe (F-12) clone gives robust, clean and specific results in WB. An excellent choice!
Date published: 2017-01-12
Rated 5 out of 5 by from Produced positive Western Blot data of ALKProduced positive Western Blot data of ALK expression in HuT 78, U-937, Raji, K-562, MDA-MB-231 and MDA-MB-435S whole cell lysates. -SCBT QC
Date published: 2015-01-27
Rated 5 out of 5 by from Nice Western Blot data of ALK expressionNice Western Blot data of ALK expression in Jurkat whole cell lysate. -SCBT QC
Date published: 2013-08-03
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ALK 抗体 (F-12) is rated 4.9 out of 5 by 7.
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