Date published: 2025-9-7
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Aldehyde dehydrogenase 1-A1/ALDH1A1 Antibody (B-5) - Western Blotting - Image 148775
ALDH1A1 抗体 (B-5): sc-374149. Hep G2 (A) 和 K-562 (B) 全细胞裂解液中 ALDH1A1 表达的 Western 印迹分析.
ALDH1A1 抗体 (B-5): sc-374149. Hep G2 (A) 和 K-562 (B) 全细胞裂解液中 ALDH1A1 表达的 Western 印迹分析.

Aldehyde dehydrogenase 1-A1/ALDH1A1 抗体 (B-5): sc-374149

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说明书
  • Aldehyde dehydrogenase 1-A1/ALDH1A1 抗体 B-5 是小鼠单克隆 IgG2a κ，Aldehyde dehydrogenase 1-A1/ALDH1A1抗体, 在29篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的ALDH1A1的N-terminus附近的氨基酸105-137之间
  • Aldehyde dehydrogenase 1-A1/ALDH1A1 抗体 (B-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 ALDH1A1
  • 抗Aldehyde dehydrogenase 1-A1/ALDH1A1抗体（B-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Aldehyde dehydrogenase 1-A1/ALDH1A1 (B-5): sc-374149的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 Aldehyde dehydrogenase 1-A1/ALDH1A1 抗体 (B-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Aldehyde dehydrogenase 1-A1/ALDH1A1 抗体 (B-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 ALDH 抗体 包括 ALDH1, ALDH16A1, ALDH18A1, ALDH1A1, ALDH1A2, ALDH1B1, ALDH1L1, ALDH2, ALDH3A1和ALDH3A2

ALDH1A1抗体（B-5）是一种IgG2a κ小鼠单克隆ALDH1A1抗体（也被称为ALDH1抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的ALDH1A1蛋白。ALDH1A1抗体（B-5）既有非偶联的抗ALDH1A1抗体形式，也有多种偶联形式的抗ALDH1A1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。醛脱氢酶（ALDHs）在酒精衍生的乙醛解毒过程中介导NADP+依赖的醛氧化为酸的过程；还参与皮质类固醇、生物源性胺和神经递质的代谢过程；以及脂质过氧化过程。ALDH1A1，也被称为视黄醛脱氢酶1（RalDH1或RALDH1）、醛脱氢酶家族1成员A1、胞质醛脱氢酶、ALDHII、ALDH-E1或ALDH E1，是一种参与视黄酸（RA）生物合成的视黄醛脱氢酶。ALDH1主要有两种肝脏异构体，可定位于胞质或线粒体空间。ALDH1A2（RALDH2、RALDH2-T）基因可产生三种不同的转录本，并催化视黄醛合成RA。ALDH1A3（ALDH6、RALDH3、ALDH1A6）是一个由13个外显子组成的37kb基因，可产生一个在唾液腺、胃和肾脏中最丰富的主要转录本，大小约为3.5kb。ALDH3A1（胃型、ALDH3、ALDHIII）可形成一种细胞质同二聚体，优先氧化芳香醛底物。作为氧化应激反应的一部分，ALDH基因上调，似乎在某些加速化疗药物代谢的肿瘤中含量丰富。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Aldehyde dehydrogenase 1-A1/ALDH1A1 抗体 (B-5) 参考文献:

  1. 选择性醛脱氢酶1A1（ALDH1A1）抑制剂的发现和开发。  |  Li, B., et al. 2021. Eur J Med Chem. 209: 112940. PMID: 33328099
  2. 新型二硫仑衍生物作为ALDH1a1选择性抑制剂。  |  Omran, Z. 2022. Molecules. 27: PMID: 35056791
  3. 癌症中的ALDH1A1：双向功能、耐药性和调节机制。  |  Yue, H., et al. 2022. Front Oncol. 12: 918778. PMID: 35814382
  4. ALDH1A1表达在早发性大肠癌中富集。  |  Venugopal, A., et al. 2022. Gastroenterology. 163: 1679-1681.e1. PMID: 35985512
  5. ALDH1A1通过增强视黄酸受体介导的DNA聚合酶θ表达，促进对PARP抑制剂的耐药性。  |  Lavudi, K., et al. 2023. NPJ Precis Oncol. 7: 66. PMID: 37429899
  6. 赖氨酸-260 2-羟基异丁酰化修饰可破坏ALDH1A1的稳定性，从而调节膀胱癌的进展。  |  Zhang, Z., et al. 2023. iScience. 26: 108142. PMID: 37867947
  7. 自闭症患者SHANK2BY29X突变影响ALDH1A1负多巴胺神经元的发育。  |  Lai, W., et al. 2024. Mol Psychiatry. 29: 3180-3194. PMID: 38704506
  8. ALDH1A1通过ZBTB7B-糖酵解途径促进肿瘤细胞的免疫逃逸。  |  Wang, M., et al. 2024. Cell Death Dis. 15: 568. PMID: 39107297
  9. ALDH1A1在胶质母细胞瘤增殖和侵袭中的作用。  |  Huang, YK., et al. 2024. Chem Biol Interact. 402: 111202. PMID: 39128802
  10. 通过铁死亡靶向ALDH1A1，提高KRAS靶向疗法的有效性。  |  Bian, Y., et al. 2024. Redox Biol. 77: 103361. PMID: 39317105

订购信息

产品名称产品编号规格价格数量收藏夹

Aldehyde dehydrogenase 1-A1/ALDH1A1 抗体 (B-5)

sc-374149
200 µg/ml
$316.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 (B-5): m-IgG Fc BP-HRP 套装

sc-529674
200 µg Ab; 10 µg BP
$354.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 (B-5): m-IgGκ BP-HRP 套装

sc-522639
200 µg Ab, 40 µg BP
$354.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 (B-5): m-IgG2a BP-HRP 套装

sc-547358
200 µg Ab; 10 µg BP
$354.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 抗体 (B-5) AC

sc-374149 AC
500 µg/ml, 25% agarose
$416.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 抗体 (B-5) HRP

sc-374149 HRP
200 µg/ml
$316.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 抗体 (B-5) FITC

sc-374149 FITC
200 µg/ml
$330.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 抗体 (B-5) PE

sc-374149 PE
200 µg/ml
$343.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 抗体 (B-5) Alexa Fluor® 488

sc-374149 AF488
200 µg/ml
$357.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 抗体 (B-5) Alexa Fluor® 546

sc-374149 AF546
200 µg/ml
$357.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 抗体 (B-5) Alexa Fluor® 594

sc-374149 AF594
200 µg/ml
$357.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 抗体 (B-5) Alexa Fluor® 647

sc-374149 AF647
200 µg/ml
$357.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 抗体 (B-5) Alexa Fluor® 680

sc-374149 AF680
200 µg/ml
$357.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 抗体 (B-5) Alexa Fluor® 790

sc-374149 AF790
200 µg/ml
$357.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 (B-5) 中和勝肽

sc-374149 P
100 µg/0.5 ml
$68.00

Can this antibody be used for IF in 4% PFA fixed rat brain tissue

Asked by: bob27
Thank you for your question. sc-374149ALDH1A1 (B-5) can be used for IF in 4% PFA fixed mouse, rat or human samples.
Answered by: BlakeJ
Date published: 2025-05-14

Is this product (sc-374149) conjugated with any fluorescein like FITC or PE?

Asked by: Lina_xu
Thank you for your question. Anti-Aldehyde dehydrogenase 1-A1/ALDH1A1 Antibody (B-5): sc-374149 is unconjugated. For a FITC- or PE- conjugated version of this antibody, please indicate the product code 'sc-374149 FITC' or 'sc-374149 PE', respectively, when you place an order.
Answered by: Tech Support Europe
Date published: 2022-02-21

I would like to use Alexa fluor 488-labeled anti-ALDH1 product (SC-374149 AF488) for flow cytometry. Can you let me know the corresponding isotype antibody product of this antibody?

Asked by: govltpfkj
Thank you for your question. Anti-Aldehyde dehydrogenase 1-A1/ALDH1A1 Antibody (B-5) is a mouse monoclonal IgG2a, so the appropriate isotype control for the AF488 conjugate would be normal mouse IgG2a Alexa Fluor® 488: sc-3891.
Answered by: Tech Support Europe
Date published: 2021-11-02

Can this product (FITC conjugated) be used for flow cytometry?

Asked by: govltpfk
Thank you for your question. Our FITC conjugated antibodies can be used for Immunofluorescence Cell Staining and Flow Cytometry.
Answered by: Tech Support Europe
Date published: 2021-10-14

I'm getting strong band just below 75.Could you pls tell me what its likely to be. Sometimes I see multiple bands as well, its hard for me to make conclusion. FYI - these are protein lysates of rhabdomyosarcoma. Whats its exact molecular wt

Asked by: Alamelu
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-01-26

can this product be used to check WB，IHC in mouse？

Asked by: z wh
Thanks for your question. Yes, this antibody is mouse, rat and human reactive.
Answered by: Technical Support
Date published: 2018-12-14

In human liver tissue extracts ALDH1A1 shows two bands! Can you tell me what is the explanation for that? Thank you!

Asked by: 05Anca
Dear Anca, thank you very much for your question. The MW is 56 kDa. For more details about your bands which kDa and your protocol, please contact our European Office by email europe@scbt.com or phone: toll-free 00800 4573 8000.
Answered by: Technical Support Europe
Date published: 2017-09-26

What application is the blocking peptide sc-374149 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from ottimo funzionamentoOttimi risultati in WB su linee di CRC: HCT15 e HCT116
Date published: 2024-12-09
Rated 4 out of 5 by from Promising IHC staining resultI got a promising IHC staining result with DAB in mouse liver
Date published: 2019-11-22
Rated 5 out of 5 by from Very goodI used it in IF, on hiPSCs-derived dopaminergic neurons differentiated in vitro: fixation with 4% PFA for 15' at RT; permeabilization 0,3% triton for 15'; blocking with 3% BSA, 2% Donkey serum, 0,1% tween for 1' and 1/2. I used a dilution 1:100 and the signal is high and without background
Date published: 2019-03-18
Rated 5 out of 5 by from ALDH1A1 antibody B-5Excelent antibody for western blot. Detected ALDH1A1 at expected size in 15 ug total proteine lysate, wet transfer one hour at 450 mAmps on PVDF membrane, overnight blocking with 5% nonfat dry milk in PBS, one hour incubation at RT with 1:500 anti ALDH1A1 in 5% PBS with 0,2%Tween followed by one hour incubation with 1:10.000 anti mouse (sc-358914) HRP conjugated antibody and detection with ECL prime substrate followed by short film exposure (under 1 minute) and automatic processing.
Date published: 2017-09-25
Rated 3 out of 5 by from Medium features in western blot assay.Blocking with milk for an hour. Primary antibody 1/500 PBST over night at 4ºC + anti-mouse for 1 hour. Performed western blot in 3 different breast cancer cell lines. Visible background, undefined band.
Date published: 2017-09-16
Rated 5 out of 5 by from Good for WBStrong and single band in WB at 1:500, UM-UC-3,T24 and 5637 cells
Date published: 2017-09-11
Rated 4 out of 5 by from Good result in total lysate human cell linesWe observed a marked band at approx. 60kDa and also a lower band which we haven't determined what it is. Blocking in milk for an hour; 1/1000 ALDH1A1 in PBS-T 1% at 4ºC ON; secondary atb 1/4000.
Date published: 2017-06-30
Rated 5 out of 5 by from The antibody quality is very goodMethods The cellular localization of ALDH1A1 Antibody in mouse kidney tissue was detected by immunoblot and immunohistochemistry. We found that Western Blotting can usually be diluted with 1:500.
Date published: 2017-02-09
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Aldehyde dehydrogenase 1-A1/ALDH1A1 抗体 (B-5) is rated 4.7 out of 5 by 12.
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