Date published: 2025-9-8

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adenosine deaminase 抗体 (D-4): sc-28346

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说明书
  • adenosine deaminase 抗体 D-4 是小鼠单克隆 IgG1 κ,adenosine deaminase抗体, 在2篇文献中引用,规格为200 µg/ml
  • 免疫human物种的adenosine deaminase的氨基酸64-363
  • adenosine deaminase 抗体 (D-4) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 adenosine deaminase
  • 抗adenosine deaminase抗体(D-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取adenosine deaminase (D-4): sc-28346免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 adenosine deaminase 抗体 (D-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与adenosine deaminase 抗体 (D-4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

腺苷脱氨酶抗体(D-4)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)检测人源腺苷脱氨酶。抗腺苷脱氨酶抗体(D-4)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。腺苷脱氨酶在嘌呤回收途径中起着至关重要的作用,它催化腺苷脱氨生成肌苷,而肌苷对于调节细胞外腺苷水平至关重要。腺苷水平过高会对淋巴细胞产生毒性,因此,腺苷脱氨酶活性对于维持免疫稳态至关重要。此外,腺苷脱氨酶与CD26相互作用,影响T细胞中的共刺激信号,这对于有效的免疫反应至关重要。腺苷脱氨酶缺乏会导致严重的免疫缺陷疾病,如严重联合免疫缺陷(SCID),并可能与遗传性溶血性贫血有关,因为腺苷脱氨酶水平会急剧上升。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

adenosine deaminase 抗体 (D-4) 参考文献:

  1. 腺苷脱氨酶:功能意义和不同类别的抑制剂。  |  Cristalli, G., et al. 2001. Med Res Rev. 21: 105-28. PMID: 11223861
  2. A3腺苷受体信号转导有助于腺苷脱氨酶缺陷小鼠的气道炎症和粘液产物。  |  Young, HW., et al. 2004. J Immunol. 173: 1380-9. PMID: 15240734
  3. 腺苷脱氨酶在自身免疫性疾病中的作用。  |  Gao, ZW., et al. 2021. Autoimmun Rev. 20: 102709. PMID: 33197575
  4. 晕厥患者中的腺苷、腺苷脱氨酶和植入式记录仪结果。  |  Mitro, P., et al. 2022. Pacing Clin Electrophysiol. 45: 768-772. PMID: 35502914
  5. 早期发现腺苷脱氨酶缺乏症与过度诊断风险之间的平衡。  |  Revak, S., et al. 2022. Ann Allergy Asthma Immunol. 129: 665-666. PMID: 36464395
  6. 腺苷脱氨酶在前列腺癌进展中的作用。  |  Charles, C., et al. 2023. Am J Clin Exp Urol. 11: 594-612. PMID: 38148936
  7. 人类腺苷脱氨酶正常形态和突变形态分析综述  |  Daddona, PE. and Kelley, WN. 1980. Mol Cell Biochem. 29: 91-101. PMID: 6988697
  8. 与遗传性溶血性贫血缔合作用相关的红细胞酶病的分子基础:突变酶表。  |  Miwa, S. and Fujii, H. 1996. Am J Hematol. 51: 122-32. PMID: 8579052
  9. SCID:腺苷脱氨酶缺乏症的作用。  |  Resta, R. and Thompson, LF. 1997. Immunol Today. 18: 371-4. PMID: 9267078
  10. 确定 CD26 上的腺苷脱氨酶结合域及其对 T 细胞活化的免疫调节作用。  |  Dong, RP., et al. 1997. J Immunol. 159: 6070-6. PMID: 9550406
  11. 外腺苷脱氨酶在淋巴细胞中的酶促和酶外作用  |  Franco, R., et al. 1998. Immunol Rev. 161: 27-42. PMID: 9553762
  12. CD26 在 T 细胞免疫反应中的结构和功能。  |  Morimoto, C. and Schlossman, SF. 1998. Immunol Rev. 161: 55-70. PMID: 9553764

订购信息

产品名称产品编号规格价格数量收藏夹

adenosine deaminase 抗体 (D-4)

sc-28346
200 µg/ml
$316.00

adenosine deaminase (D-4): m-IgG Fc BP-HRP 套装

sc-528429
200 µg Ab; 10 µg BP
$354.00

adenosine deaminase (D-4): m-IgGκ BP-HRP 套装

sc-520804
200 µg Ab, 40 µg BP
$354.00

adenosine deaminase (D-4): m-IgG1 BP-HRP 套装

sc-542929
200 µg Ab; 20 µg BP
$354.00

adenosine deaminase 抗体 (D-4) AC

sc-28346 AC
500 µg/ml, 25% agarose
$416.00

adenosine deaminase 抗体 (D-4) HRP

sc-28346 HRP
200 µg/ml
$316.00

adenosine deaminase 抗体 (D-4) FITC

sc-28346 FITC
200 µg/ml
$330.00

adenosine deaminase 抗体 (D-4) PE

sc-28346 PE
200 µg/ml
$343.00

adenosine deaminase 抗体 (D-4) Alexa Fluor® 488

sc-28346 AF488
200 µg/ml
$357.00

adenosine deaminase 抗体 (D-4) Alexa Fluor® 546

sc-28346 AF546
200 µg/ml
$357.00

adenosine deaminase 抗体 (D-4) Alexa Fluor® 594

sc-28346 AF594
200 µg/ml
$357.00

adenosine deaminase 抗体 (D-4) Alexa Fluor® 647

sc-28346 AF647
200 µg/ml
$357.00

adenosine deaminase 抗体 (D-4) Alexa Fluor® 680

sc-28346 AF680
200 µg/ml
$357.00

adenosine deaminase 抗体 (D-4) Alexa Fluor® 790

sc-28346 AF790
200 µg/ml
$357.00

Anti-adenosine deaminase Antibody (D-4) is an antibody that detects adenosine deaminase of human origin. Which of the two? ADA-1 or ADA-2 Sincerely Jacobus

Asked by: Jacobus
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2024-12-19

This was a suggested replacement product for the previous anti-mouse ADA antibody. Does this monoclonal detect murine ADA?

Asked by: mADA
Thank you for your question. This antibody has not yet been tested in mouse and so that is not a recommended species at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2023-03-03

Can adenosine deaminase (D-4): sc-28346 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, adenosine deaminase (D-4): sc-28346 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 2 out of 5 by from western blot通过七种不同细胞的使用情况表明,完全适用于人源的不同细胞系A549、Hela、Jurkat等的Western Blot
Date published: 2017-07-21
Rated 5 out of 5 by from Exceptional immunoperoxidase membrane stainingExceptional immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human cerebellum tissue. -SCBT QC
Date published: 2015-03-25
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adenosine deaminase 抗体 (D-4) is rated 4.0 out of 5 by 3.
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