Date published: 2025-9-6
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Adducin γ Antibody (G-2) - Western Blotting - Image 288283
Adducin γ 抗体 (G-2): sc-365177. F9 (A), HeLa (B) 和 K-562 (C) 全细胞裂解液中 Adducin γ 表达的 Western 印迹分析.
Adducin γ 抗体 (G-2): sc-365177. F9 (A), HeLa (B) 和 K-562 (C) 全细胞裂解液中 Adducin γ 表达的 Western 印迹分析.

Adducin γ 抗体 (G-2): sc-365177

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寫評論提問

说明书
  • Adducin gamma 抗体 G-2 是小鼠单克隆 IgG1 κ，Adducin γ抗体, 在3篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Adducin γ的C-terminus附近的氨基酸之间
  • Adducin gamma 抗体 (G-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Adducin γ
  • 抗Adducin gamma抗体（G-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Adducin γ (G-2): sc-365177的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Adducin γ 抗体 (G-2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

此外 Adducin 抗体 包括 Adducin α, Adducin β和Adducin γ

Adducin γ抗体（G-2）是一种IgG1 κ小鼠单克隆Adducin γ抗体（也称为γ-adducin抗体或ADD3抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的Adducin γ蛋白。Adducin γ抗体（G-2）既可作为非共轭抗Adducin γ抗体形式，也可作为多种共轭形式的抗Adducin γ抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。Adducins是由三个基因（α、β、γ）编码的一类细胞骨架蛋白。Adducin是一种与质膜内小叶相关的蛋白质，也是定位于膜骨架血影蛋白-肌动蛋白连接处的蛋白质之一。在细胞凋亡过程中，皮质肌动蛋白细胞骨架网络会丢失，而Adducins在皮质肌动蛋白网络组织中起核心作用。Adducin α是一种细胞骨架蛋白，参与肾小管中的钠泵活动，并与高血压相关。与Adducin β的受限表达相比，Adducin α和Adducin γ的表达无处不在。在人类中，Adducin β在大脑和造血组织（如骨髓）中高水平表达，而在小鼠中则在脾脏中高水平表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Adducin γ 抗体 (G-2) 参考文献:

  1. 与高血压有关的大脑γ-adducin表达减少。  |  Yang, H., et al. 2002. Circ Res. 91: 633-9. PMID: 12364392
  2. 靶向缺失α-ducin会导致小鼠缺失β-和γ-ducin、代偿性溶血性贫血和致死性脑积水。  |  Robledo, RF., et al. 2008. Blood. 112: 4298-307. PMID: 18723693
  3. 小鼠γ-ducin基因（Add3）的定向缺失揭示了红细胞和非红细胞中α-ducin相互作用的差异。  |  Sahr, KE., et al. 2009. Am J Hematol. 84: 354-61. PMID: 19425068
  4. γ-Adducin刺激对噻嗪敏感的NaCl共转运体。  |  Dimke, H., et al. 2011. J Am Soc Nephrol. 22: 508-17. PMID: 21164023
  5. γadducin突变与遗传性脑瘫有关。  |  Kruer, MC., et al. 2013. Ann Neurol. 74: 805-14. PMID: 23836506
  6. γ-adducin在荚膜病理生理学中肌动蛋白细胞骨架重排中的作用  |  Gao, W., et al. 2021. Am J Physiol Renal Physiol. 320: F97-F113. PMID: 33308016
  7. 敲除γ-Adducin可促进NG-硝基-L-精氨酸-甲基酯诱导的高血压肾损伤  |  Fan, L., et al. 2021. J Pharmacol Exp Ther. 377: 189-198. PMID: 33414130
  8. 一种γ-adducin裂解片段可诱导阿尔茨海默病的神经元缺陷和突触功能障碍。  |  Xiong, M., et al. 2021. Prog Neurobiol. 203: 102074. PMID: 33992672
  9. 转录后抑制γ-adducin可促进骨肉瘤细胞的增殖和迁移。  |  Suo, Z., et al. 2022. Tumori. 108: 600-608. PMID: 34632867
  10. γ-Adducin 1-357 片段可促进 tau 病理学。  |  Yu, H., et al. 2023. Front Aging Neurosci. 15: 1241750. PMID: 37771520

订购信息

产品名称产品编号规格价格数量收藏夹

Adducin γ 抗体 (G-2)

sc-365177
200 µg/ml
$316.00

Adducin γ 抗体 (G-2) AC

sc-365177 AC
500 µg/ml, 25% agarose
$416.00

Adducin γ 抗体 (G-2) HRP

sc-365177 HRP
200 µg/ml
$316.00

Adducin γ 抗体 (G-2) FITC

sc-365177 FITC
200 µg/ml
$330.00

Adducin γ 抗体 (G-2) PE

sc-365177 PE
200 µg/ml
$343.00

Adducin γ 抗体 (G-2) Alexa Fluor® 488

sc-365177 AF488
200 µg/ml
$357.00

Adducin γ 抗体 (G-2) Alexa Fluor® 546

sc-365177 AF546
200 µg/ml
$357.00

Adducin γ 抗体 (G-2) Alexa Fluor® 594

sc-365177 AF594
200 µg/ml
$357.00

Adducin γ 抗体 (G-2) Alexa Fluor® 647

sc-365177 AF647
200 µg/ml
$357.00

Adducin γ 抗体 (G-2) Alexa Fluor® 680

sc-365177 AF680
200 µg/ml
$357.00

Adducin γ 抗体 (G-2) Alexa Fluor® 790

sc-365177 AF790
200 µg/ml
$357.00

Adducin γ (G-2) 中和勝肽

sc-365177 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-365177 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 7
Date published: 2017-01-17
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Rated 1 out of 5 by from No signal on westernI could not get this antibody to work on a western blot using whole cell lysate from breast cancer cell lines. I tried various concentrations and an overnight exposure. The protein is highly expressed in these cells as determined using LC-MS/MS.
Date published: 2017-11-17
Rated 5 out of 5 by from Worked greatUsed for neonatal rat ventricular myocytes at 1:250 dilution in western blot. Gave a nice, crisp band.
Date published: 2017-10-04
Rated 5 out of 5 by from Works great for WBAdducin γ (G-2) gave a strong band at ~90 kDa in HeLa Whole Cell Lysate: sc-2200.
Date published: 2017-01-20
Rated 3 out of 5 by from Reasonably potent and highly specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was detection of the ~94 kDa target protein in an A431/HepG2/MCF7 cell lysate mix, but not in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested or in the A431/HepG2/MCF7 cell lysate mix.
Date published: 2016-11-10
Rated 5 out of 5 by from StrongStrong, specific band obtained in WB in F9, HeLa and K-562 whole cell lysates. -SCBT QC
Date published: 2015-05-15
Rated 5 out of 5 by from Produced exceptional immunofluorescence membraneProduced exceptional immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-18
Rated 5 out of 5 by from Performed well in WB in mouse Adducin transfectedPerformed well in WB in mouse Adducin transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-01-18
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Adducin γ 抗体 (G-2) is rated 4.1 out of 5 by 7.
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