Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Adducin α Antibody (4D1) - Western Blotting - Image 45683
Adducin α (4D1): sc-33633. T98G (A), H4 (B), KNRK (C) 和 C6 (D) 全细胞裂解液中 Adducin α 表达的 Western 印迹分析.
Adducin α (4D1): sc-33633. T98G (A), H4 (B), KNRK (C) 和 C6 (D) 全细胞裂解液中 Adducin α 表达的 Western 印迹分析.

Adducin α 抗体 (4D1): sc-33633

4.7(6)
寫評論提問

说明书
  • Adducin alpha 抗体 4D1 是小鼠单克隆 IgG1 κ，Adducin α抗体, 在1篇文献中引用，规格为200 µg/ml
  • 免疫rat物种的recombinant Adducin α的氨基酸181-245
  • Adducin alpha 抗体 (4D1) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 Adducin α
  • 抗Adducin alpha抗体（4D1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Adducin α (4D1): sc-33633的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Adducin α 抗体 (4D1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Adducin α 抗体 (4D1) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Adducin 抗体 包括 Adducin α, Adducin β和Adducin γ

Adducin α 抗体（4D1）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类来源的 Adducin α 蛋白。抗Adducin α抗体（4D1）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Adducin是由三个基因（α、β、γ）编码的细胞骨架蛋白家族，其中Adducin α在组织皮质肌动蛋白细胞骨架网络方面发挥着关键作用，该网络可维持细胞形状和完整性。Adducin α主要定位于质膜的spectrin-actin交界处，有助于结构稳定并参与细胞信号传导和运动。Adducin α的重要性还体现在它与肾小管钠泵活性之间的关联上，从而将Adducin α与高血压这一重大健康问题联系起来。虽然Adducin α和Adducin γ的表达无处不在，但Adducin β主要存在于大脑和造血组织中，这凸显了这些蛋白质在不同细胞环境中的不同作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Adducin α 抗体 (4D1) 参考文献:

  1. 靶向干扰 beta adducin 基因（Add2）会导致小鼠红细胞球形细胞增多症。  |  Gilligan, DM., et al. 1999. Proc Natl Acad Sci U S A. 96: 10717-22. PMID: 10485892
  2. 正常血压双胞胎的血压与α-胰岛素基因座存在缔合作用，但缺乏关联性。  |  Busjahn, A., et al. 1999. J Hypertens. 17: 1437-41. PMID: 10526904
  3. α-胰高血糖素多态性与血压和心肌梗死风险的关系。  |  Psaty, BM., et al. 2000. J Hum Hypertens. 14: 95-7. PMID: 10723114
  4. 在顺铂诱导的肾上皮细胞凋亡过程中，caspase-3先在Asp-Asp-Ser-Asp633-Ala处对肌动蛋白盖蛋白α-adducin进行裂解，然后在丝氨酸726上对其进行磷酸化。  |  van de Water, B., et al. 2000. J Biol Chem. 275: 25805-13. PMID: 10823823
  5. 轻度球形红细胞遗传性椭圆形细胞增多症和β-adducin缺陷小鼠体内α-和γ-adducins水平的改变  |  Muro, AF., et al. 2000. Blood. 95: 3978-85. PMID: 10845937
  6. adducinα-亚基中与高血压相关的突变会导致更高的AP2-mu2磷酸化，并影响Na+,K+-ATP酶对GPCR信号和细胞内钠的转运。  |  Efendiev, R., et al. 2004. Circ Res. 95: 1100-8. PMID: 15528469
  7. 高血压：分子方法。  |  Lele, RD. 2004. J Assoc Physicians India. 52: 53-62. PMID: 15633721
  8. 分析正常和恶性人类卵巢表面上皮细胞中雌激素调控基因表达的变化。  |  Syed, V., et al. 2005. Oncogene. 24: 8128-43. PMID: 16116479
  9. C2C12 肌细胞在 SHIP2 缺失和胰岛素刺激下的转录物分析。  |  Huard, C., et al. 2007. Genomics. 89: 270-9. PMID: 17123777
  10. 人类 alpha 和 beta adducin 的一级结构和结构域组织。  |  Joshi, R., et al. 1991. J Cell Biol. 115: 665-75. PMID: 1840603
  11. adducinα和β亚基中与高血压相关的点突变会影响肌动蛋白细胞骨架和离子转运。  |  Tripodi, G., et al. 1996. J Clin Invest. 97: 2815-22. PMID: 8675693
  12. Rabphilin-3A：突触囊泡交通的多功能调节器。  |  Burns, ME., et al. 1998. J Gen Physiol. 111: 243-55. PMID: 9450942

订购信息

产品名称产品编号规格价格数量收藏夹

Adducin α 抗体 (4D1)

sc-33633
200 µg/ml
$316.00

Adducin α (4D1): m-IgG Fc BP-HRP 套装

sc-528477
200 µg Ab; 10 µg BP
$354.00

Adducin α (4D1): m-IgGκ BP-HRP 套装

sc-520867
200 µg Ab, 40 µg BP
$354.00

Adducin α (4D1): m-IgG1 BP-HRP 套装

sc-542955
200 µg Ab; 20 µg BP
$354.00

Adducin α 抗体 (4D1) AC

sc-33633 AC
500 µg/ml, 25% agarose
$416.00

Adducin α 抗体 (4D1) HRP

sc-33633 HRP
200 µg/ml
$316.00

Adducin α 抗体 (4D1) FITC

sc-33633 FITC
200 µg/ml
$330.00

Adducin α 抗体 (4D1) PE

sc-33633 PE
200 µg/ml
$343.00

Adducin α 抗体 (4D1) Alexa Fluor® 488

sc-33633 AF488
200 µg/ml
$357.00

Adducin α 抗体 (4D1) Alexa Fluor® 546

sc-33633 AF546
200 µg/ml
$357.00

Adducin α 抗体 (4D1) Alexa Fluor® 594

sc-33633 AF594
200 µg/ml
$357.00

Adducin α 抗体 (4D1) Alexa Fluor® 647

sc-33633 AF647
200 µg/ml
$357.00

Adducin α 抗体 (4D1) Alexa Fluor® 680

sc-33633 AF680
200 µg/ml
$357.00

Adducin α 抗体 (4D1) Alexa Fluor® 790

sc-33633 AF790
200 µg/ml
$357.00

I need to use an antibody to detect the protein in samples of equine origin. Do you know if sc-33633: Adducin α (4D1) mouse monoclonal antibody will work?

Asked by: Professor Griffin
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_33633, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 107ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from SpecificI requested the trial-size sample of this antibody, to use for IHC with formalin-fixed, paraffin-embedded tissues. Reaction product was specific in heart sections. Also, a crisp band appeared on a Western blot with heart lysate.
Date published: 2017-09-21
Rated 5 out of 5 by from GreatVery happy. Ab worked very good for mouse spleen WB.
Date published: 2017-03-20
Rated 4 out of 5 by from Potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium detection of the ~120 kDa target in mouse tissues (brain/testes/thymus/muscle mix), and no detection of the target protein in a HeLa/Jurkat cell lysate mix. No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested.
Date published: 2016-11-10
Rated 5 out of 5 by from Performed well in WB in mouse Adducin transfectedPerformed well in WB in mouse Adducin transfected 293T and T98G whole cell lysates. -SCBT QC
Date published: 2015-05-26
Rated 5 out of 5 by from Strong positive band with good specificityStrong positive band with good specificity observed in WB in T98G, H4, KNRK and C6 whole cell lysates. -SCBT QC
Date published: 2015-04-21
Rated 5 out of 5 by from Produced very strong band in WB in rat brainProduced very strong band in WB in rat brain and rat spleen tissue extracts and LADMAC and T98G whole cell lysates. -SCBT QC
Date published: 2014-03-01
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_33633, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 13ms
  • REVIEWS, PRODUCT
Adducin α 抗体 (4D1) is rated 4.7 out of 5 by 6.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_33633, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 97ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。