Date published: 2025-9-7
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Nicotinic Acetylcholine Receptor epsilon/CHRNE Antibody (B-11) - Western Blotting - Image 301133
AChRε 抗体 (B-11): sc-376747. NIH/3T3 (A), SH-SY5Y (B), Hep G2 (C) 和 HeLa (D) 全细胞裂解液及人骨骼肌组织提取物 (E) 中 AChRε 表达的 Western 印迹分析.
Nicotinic Acetylcholine Receptor epsilon/CHRNE Antibody (B-11) - Western Blotting - Image 301133
AChRε 抗体 (B-11): sc-376747. NIH/3T3 (A), SH-SY5Y (B), Hep G2 (C) 和 HeLa (D) 全细胞裂解液及人骨骼肌组织提取物 (E) 中 AChRε 表达的 Western 印迹分析.
Nicotinic Acetylcholine Receptor epsilon/CHRNE Antibody (B-11) - Western Blotting - Image 158463
AChRε (B-11): sc-376747. BC3H1 (A) 和 SH-SY5Y (B) 全细胞裂解液中 AChRε 表达的 Western 印迹分析.
Nicotinic Acetylcholine Receptor epsilon/CHRNE Antibody (B-11) - Western Blotting - Image 374463
AChRε 抗体 (B-11): sc-376747. BC3H1 (A) 和 3T3-L1 (B) 全细胞裂解液中 AChRε 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
AChRε 抗体 (B-11): sc-376747. NIH/3T3 (A), SH-SY5Y (B), Hep G2 (C) 和 HeLa (D) 全细胞裂解液及人骨骼肌组织提取物 (E) 中 AChRε 表达的 Western 印迹分析.

Nicotinic Acetylcholine Receptor epsilon/CHRNE 抗体 (B-11): sc-376747

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寫評論提問

说明书
  • Nicotinic Acetylcholine Receptor epsilon/CHRNE 抗体 B-11 是小鼠单克隆 IgG1 κ，Nicotinic Acetylcholine Receptor epsilon/CHRNE抗体, 在4篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列334-441位于human物种的AChRε的C-terminus
  • Nicotinic Acetylcholine Receptor epsilon/CHRNE 抗体 (B-11) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 AChRε
  • 抗Nicotinic Acetylcholine Receptor epsilon/CHRNE抗体（B-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Nicotinic Acetylcholine Receptor epsilon/CHRNE (B-11): sc-376747的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是Nicotinic Acetylcholine Receptor epsilon/CHRNE Antibody (B-11) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与Nicotinic Acetylcholine Receptor epsilon/CHRNE Antibody (B-11) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外AChR抗体，包括AChR α, AChR α 1, AChR α 2, AChR α 3, AChR α 4, AChR α 5, AChR α 6, AChR α 7, AChR α 8, AChR α 9, AChRβ, AChRbeta1, AChRbeta2, AChR δ, AChR ε和AChR γ

AChRε抗体（B-11）是一种IgG1 κ小鼠单克隆AChRε抗体（也称为CHRNE抗体、ACHRE抗体或烟碱型乙酰胆碱受体ε抗体），可通过WB、IP、IF和ELISA方法检测小鼠、大鼠和人类来源的AChRε蛋白。AChRε抗体（B-11）既有非偶联形式的抗AChRε抗体，也有多种偶联形式的抗AChRε抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。配体门控离子通道受体家族的成员以其对神经递质的快速传递反应为特征。该家族的两个重要成员是烟碱型乙酰胆碱受体和谷氨酸受体，它们都由五个同源亚基组成，形成一个跨膜水孔。这些跨膜受体在响应其同源神经递质时会改变构象。烟碱型乙酰胆碱受体（AChRs）位于神经肌肉接头的突触后膜上，与乙酰胆碱分子结合，允许离子通过孔道。谷氨酸受体存在于中枢神经系统细胞的突触后膜上。乙酰胆碱与乙酰胆碱受体结合在突触处产生的活动会被乙酰胆碱酯酶终止，乙酰胆碱酯酶是一种能迅速水解乙酰胆碱的酶。AChRε，也称为CMS1D、CMS1E、CMS2A、FCCMS、SCCMS或CHRNE，是一种493个氨基酸的多次跨膜蛋白，与先天性肌无力综合征慢通道型（SCCMS）、先天性肌无力综合征快通道型（FCCMS）和乙酰胆碱受体缺乏型先天性肌无力综合征相关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Nicotinic Acetylcholine Receptor epsilon/CHRNE 抗体 (B-11) 参考文献:

  1. 利用傅立叶变换红外差分光谱探测尼古丁乙酰胆碱受体的构象变化。  |  Baenziger, JE., et al. 1992. Biophys J. 62: 64-6. PMID: 1600101
  2. 大脑中的配体门控离子通道：氨基酸受体超家族。  |  Betz, H. 1990. Neuron. 5: 383-92. PMID: 1698394
  3. 乙酰胆碱酯酶再激活剂改变了尼古丁乙酰胆碱受体离子通道的功能特性。  |  Alkondon, M., et al. 1988. J Pharmacol Exp Ther. 245: 543-56. PMID: 2452874
  4. 神经递质的作用：打开配体门控离子通道。  |  Unwin, N. 1993. Cell. 72 Suppl: 31-41. PMID: 7679054
  5. 神经元烟碱乙酰胆碱受体的多样性。  |  Sargent, PB. 1993. Annu Rev Neurosci. 16: 403-43. PMID: 7681637
  6. 神经递质的定量释放和长期电位。  |  Stevens, CF. 1993. Cell. 72 Suppl: 55-63. PMID: 8094037
  7. NMDA 受体在信息处理中的作用。  |  Daw, NW., et al. 1993. Annu Rev Neurosci. 16: 207-22. PMID: 8460891
  8. α5亚基对神经元乙酰胆碱受体通道的功能贡献。  |  Ramirez-Latorre, J., et al. 1996. Nature. 380: 347-51. PMID: 8598930

订购信息

产品名称产品编号规格价格数量收藏夹

Nicotinic Acetylcholine Receptor epsilon/CHRNE 抗体 (B-11)

sc-376747
200 µg/ml
$316.00

Nicotinic Acetylcholine Receptor epsilon/CHRNE (B-11): m-IgG Fc BP-HRP 套装

sc-529929
200 µg Ab; 10 µg BP
$354.00

Nicotinic Acetylcholine Receptor epsilon/CHRNE (B-11): m-IgGκ BP-HRP 套装

sc-523078
200 µg Ab, 40 µg BP
$354.00

Nicotinic Acetylcholine Receptor epsilon/CHRNE 抗体 (B-11) AC

sc-376747 AC
500 µg/ml, 25% agarose
$416.00

Nicotinic Acetylcholine Receptor epsilon/CHRNE 抗体 (B-11) HRP

sc-376747 HRP
200 µg/ml
$316.00

Nicotinic Acetylcholine Receptor epsilon/CHRNE 抗体 (B-11) FITC

sc-376747 FITC
200 µg/ml
$330.00

Nicotinic Acetylcholine Receptor epsilon/CHRNE 抗体 (B-11) PE

sc-376747 PE
200 µg/ml
$343.00

Nicotinic Acetylcholine Receptor epsilon/CHRNE 抗体 (B-11) Alexa Fluor® 488

sc-376747 AF488
200 µg/ml
$357.00

Nicotinic Acetylcholine Receptor epsilon/CHRNE 抗体 (B-11) Alexa Fluor® 546

sc-376747 AF546
200 µg/ml
$357.00

Nicotinic Acetylcholine Receptor epsilon/CHRNE 抗体 (B-11) Alexa Fluor® 594

sc-376747 AF594
200 µg/ml
$357.00

Nicotinic Acetylcholine Receptor epsilon/CHRNE 抗体 (B-11) Alexa Fluor® 647

sc-376747 AF647
200 µg/ml
$357.00

Nicotinic Acetylcholine Receptor epsilon/CHRNE 抗体 (B-11) Alexa Fluor® 680

sc-376747 AF680
200 µg/ml
$357.00

Nicotinic Acetylcholine Receptor epsilon/CHRNE 抗体 (B-11) Alexa Fluor® 790

sc-376747 AF790
200 µg/ml
$357.00

I need to use an antibody to detect the protein in samples of avian origin. Do you know if AChRε (B-11): sc-376747 mouse monoclonal antibody will work?

Asked by: AbPolly
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from works greatThis antibody works great for WB of mouse endothelial cells. Specific band is observed at 1:800 dilution,recommend use.
Date published: 2017-08-12
Rated 5 out of 5 by from good for western blottingIn western blotting this antibody specifically recognised a cell lysate from HEK293T cells transfected with cDNA encoding the human AChR epsilon subunit. A much fainter band was visible in mock transfected cells (the protein is thought to be expressed at low levels in this cell line). Primary antibody was used 1:100 but could be diluted more as the band was visible after exposing the autoradiograph for only about 1 second.
Date published: 2016-12-15
Rated 5 out of 5 by from Great Western Blot data of AChR expressionGreat Western Blot data of AChR expression in NIH/3T3, SH-SY5Y, Hep G2 and HeLa whole cell lysates and human skeletal muscle tissue extract . -SCBT QC
Date published: 2015-03-08
Rated 5 out of 5 by from Good Western Blot data of AChRGood Western Blot data of AChR_ expression in BC3H1 and SH-SY5Y whole cell lysates. -SCBT QC
Date published: 2013-06-03
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Nicotinic Acetylcholine Receptor epsilon/CHRNE 抗体 (B-11) is rated 5.0 out of 5 by 4.
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