Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
14-3-3 ε Antibody (8C3) - Western Blotting - Image 355310
14-3-3 ε 抗体 (8C3): sc-23957. HeLa (A), SK-N-SH (B), NIH/3T3 (C), c4 (D), C6 (E) 和 A-10 (F) 全细胞裂解液中 14-3-3 ε 表达的 Western 印迹分析.
14-3-3 ε 抗体 (8C3): sc-23957. HeLa (A), SK-N-SH (B), NIH/3T3 (C), c4 (D), C6 (E) 和 A-10 (F) 全细胞裂解液中 14-3-3 ε 表达的 Western 印迹分析.

14-3-3 ε 抗体 (8C3): sc-23957

4.6(16)
寫評論提問

说明书
  • 14-3-3 epsilon 抗体 8C3 是小鼠单克隆 IgG2a κ，14-3-3 ε抗体, 在52篇文献中引用，规格为200 µg/ml
  • 针对部分纯化的松果体蛋白（ovine起源）
  • 14-3-3 epsilon 抗体 (8C3) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human 和ovine 来源的 14-3-3 ε
  • 抗14-3-3 epsilon抗体（8C3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取14-3-3 ε (8C3): sc-23957的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 14-3-3 ε 抗体 (8C3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与14-3-3 ε 抗体 (8C3) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 14-3-3 抗体 包括 14-3-3 β, 14-3-3 ε, 14-3-3 η, 14-3-3 γ, 14-3-3 σ, 14-3-3 θ, 14-3-3 ζ和pan 14-3-3

14-3-3 ε 抗体（8C3）是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠、人类和羊源性 14-3-3 epsilon 蛋白。14-3-3 ε 单克隆抗体（8C3）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。14-3-3 epsilon蛋白在调节细胞凋亡、有丝分裂信号和细胞周期检查点等细胞过程中发挥着关键作用，因此14-3-3 epsilon在癌症生物学中具有重要意义。14-3-3 epsilon蛋白主要位于细胞质中，与多种靶蛋白相互作用，促进其正常功能和定位。14-3-3蛋白在细胞质中的存在对于隔离和调节配体至关重要，这些配体通常包含14-3-3共识结合基序，并且通常在丝氨酸或苏氨酸残基上磷酸化。通过与这些磷酸化蛋白结合，14-3-3 epsilon可以调节活性、稳定性和相互作用，从而影响关键的信号通路。14-3-3 epsilon表达或功能失调可导致一系列病理状况，包括癌症，这凸显了14-3-3 epsilon在维持细胞稳态方面的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

14-3-3 ε 抗体 (8C3) 参考文献:

  1. DAL-1/蛋白 4.1B 肿瘤抑制因子的 4.1/ezrin/radixin/moesin 结构域与 14-3-3 蛋白相互作用。  |  Yu, T., et al. 2002. Biochem J. 365: 783-9. PMID: 11996670
  2. MADM 是一种新型适配蛋白，可介导髓系白血病因子 1 的 14-3-3 结合位点发生磷酸化。  |  Lim, R., et al. 2002. J Biol Chem. 277: 40997-1008. PMID: 12176995
  3. 14-3-3 癌症联系  |  Hermeking, H. 2003. Nat Rev Cancer. 3: 931-43. PMID: 14737123
  4. 拟南芥中 14-3-3 蛋白的同工酶特异性亚细胞定位似乎是由客户相互作用驱动的。  |  Paul, AL., et al. 2005. Mol Biol Cell. 16: 1735-43. PMID: 15659648
  5. 精子中的蛋白质 YWHAE（14-3-3 epsilon）对男性生育至关重要。  |  Eisa, A., et al. 2021. Andrology. 9: 312-328. PMID: 32657535
  6. 14-3-3 epsilon 是 TNFR2 受体复合物的细胞内成分，激活它可预防骨关节炎。  |  Fu, W., et al. 2021. Ann Rheum Dis. 80: 1615-1627. PMID: 34226187
  7. 柚皮素靶向巨噬细胞 TFEB-14-3-3 epsilon 接口可抑制腹主动脉瘤。  |  Jia, Y., et al. 2022. Cell Discov. 8: 21. PMID: 35228523
  8. 携带 hnRNP C 的 14-3-3 epsilon 的核-胞质穿梭促进自噬。  |  Guo, M., et al. 2023. Cancer Biol Ther. 24: 2246203. PMID: 37599448
  9. 14-3-3：信号蛋白的调节剂？  |  Morrison, D. 1994. Science. 266: 56-7. PMID: 7939645
  10. 人类 14-3-3 eta 链基因（YWHAH）的结构组织和染色体分配。  |  Muratake, T., et al. 1996. Genomics. 36: 63-9. PMID: 8812417
  11. 14-3-3 与磷酸肽结合特异性的结构基础。  |  Yaffe, MB., et al. 1997. Cell. 91: 961-71. PMID: 9428519
  12. 一种新型鞘氨醇依赖性蛋白激酶（SDK1）能特异性地使 14-3-3 蛋白的某些同工型发生磷酸化。  |  Megidish, T., et al. 1998. J Biol Chem. 273: 21834-45. PMID: 9705322

订购信息

产品名称产品编号规格价格数量收藏夹

14-3-3 ε 抗体 (8C3)

sc-23957
200 µg/ml
$316.00

14-3-3 ε (8C3): m-IgG Fc BP-HRP 套装

sc-528385
200 µg Ab; 10 µg BP
$354.00

14-3-3 ε (8C3): m-IgGκ BP-HRP 套装

sc-520751
200 µg Ab, 40 µg BP
$354.00

14-3-3 ε 抗体 (8C3) AC

sc-23957 AC
500 µg/ml, 25% agarose
$416.00

14-3-3 ε 抗体 (8C3) HRP

sc-23957 HRP
200 µg/ml
$316.00

14-3-3 ε 抗体 (8C3) FITC

sc-23957 FITC
200 µg/ml
$330.00

14-3-3 ε 抗体 (8C3) PE

sc-23957 PE
200 µg/ml
$343.00

14-3-3 ε 抗体 (8C3) Alexa Fluor® 488

sc-23957 AF488
200 µg/ml
$357.00

14-3-3 ε 抗体 (8C3) Alexa Fluor® 546

sc-23957 AF546
200 µg/ml
$357.00

14-3-3 ε 抗体 (8C3) Alexa Fluor® 594

sc-23957 AF594
200 µg/ml
$357.00

14-3-3 ε 抗体 (8C3) Alexa Fluor® 647

sc-23957 AF647
200 µg/ml
$357.00

14-3-3 ε 抗体 (8C3) Alexa Fluor® 680

sc-23957 AF680
200 µg/ml
$357.00

14-3-3 ε 抗体 (8C3) Alexa Fluor® 790

sc-23957 AF790
200 µg/ml
$357.00

Could you please teach me the amino acid sequence of "(136-152 amino acids) of ovine pineal gland"?

Asked by: やどりぎ
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Service SH
Date published: 2024-05-08

Where does this antibody, sc-23957, bind in the amino acid sequence of rat?

Asked by: HAOHAO
Thank you for your question. This antibody is raised against partially purified proteins (136-152 amino acids) of ovine pineal gland.
Answered by: Technical Service SH
Date published: 2024-05-06

I would like to use 14-3-3 ε Antibody (8C3): sc-23957 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-23957 is available conjugated to agarose, HRP, PE, FITC, Alexa Fluor 488 and Alexa Fluor 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 13
Date published: 2017-01-20

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with 14-3-3 ε Antibody (8C3): sc-23957?

Asked by: Trav11
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Service 11
Date published: 2017-01-16
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_23957, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 108ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Good for western blottingGood band was detected by western blotting using mouse heart tissue.
Date published: 2024-09-29
Rated 5 out of 5 by from Best antibodyI have validated this antibody for mouse and rat tissue in the western blotting.
Date published: 2024-05-07
Rated 5 out of 5 by from Excellent antibodyUsed the trial sample in WB at a concentration of 1:1000 on Melanoma cell lines A375 and SK-Mel-5. With Odissey scanner i obtained a really well defined band without aspecifics around 30-35kDa.
Date published: 2021-12-14
Rated 5 out of 5 by from Specific antibody on total cell extractsThe batch of antibody I have worked very well on HeLa, NIH/3T3, Jurkat and K562 total cell extracts. The signal was strong and specific.Membranes were incubated over night with a 1'000 dilution in 5% BSA, TBST.
Date published: 2017-11-16
Rated 1 out of 5 by from did not workedendogenous in 293 and overexpression of myc-14-3-3 ep was not detected. 1/1000 dil in 2% BSA TBST, overnight rxn.
Date published: 2017-03-22
Rated 5 out of 5 by from Superb western and IHC dataOutstanding western blot data; strong signal; also great for IHC
Date published: 2017-01-20
Rated 5 out of 5 by from Good qualityI got really good results for WB and IP with this antibody.
Date published: 2017-01-17
Rated 5 out of 5 by from good for stainingThis 14-3-3 epsilon antibody works great for staining.
Date published: 2017-01-12
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_16
  • loc_zh_CN, sid_23957, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 10ms
  • REVIEWS, PRODUCT
14-3-3 ε 抗体 (8C3) is rated 4.6 out of 5 by 16.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_16
  • loc_zh_CN, sid_23957, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 177ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。