UBA3 Antibody (E-5): sc-377272

UBA3 Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects UBA3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). UBA3 Antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UBA3, also known as ubiquitin-like modifier activating enzyme 3 or UBE1C, is a 463 amino acid protein that plays a crucial role in the ubiquitin-proteasome system, which is essential for maintaining cellular homeostasis. UBA3 is primarily located in the cytoplasm and nucleus, where UBA3 activates NEDD8, a ubiquitin-like protein, thereby regulating vital cellular processes such as cell division, signaling pathways, and embryogenesis. UBA3 functioning is critical, as dysregulation can lead to various diseases, including cancer, due to the accumulation of improperly folded or damaged proteins. UBA3 exists in two isoforms resulting from alternative splicing, which may contribute to functional diversity and regulatory mechanisms within different cellular contexts.

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UBA3 Antibody (E-5) References:

1. Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway.  |  Gong, L. and Yeh, ET. 1999. J Biol Chem. 274: 12036-42. PMID: 10207026
2. Ubiquitin, proteasomes, and the regulation of intracellular protein degradation.  |  Hochstrasser, M. 1995. Curr Opin Cell Biol. 7: 215-23. PMID: 7612274
3. The ubiquitin-proteasome proteolytic pathway.  |  Ciechanover, A. 1994. Cell. 79: 13-21. PMID: 7923371
4. The ubiquitin-mediated proteolytic pathway: mechanisms of recognition of the proteolytic substrate and involvement in the degradation of native cellular proteins.  |  Ciechanover, A. and Schwartz, AL. 1994. FASEB J. 8: 182-91. PMID: 8119489
5. SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box.  |  Bai, C., et al. 1996. Cell. 86: 263-74. PMID: 8706131
6. A novel protein modification pathway related to the ubiquitin system.  |  Liakopoulos, D., et al. 1998. EMBO J. 17: 2208-14. PMID: 9545234

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-UBA3 Antibody (E-5) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-UBA3 Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

UBA3 Antibody (E-5) Specifications

Alternate Product Name: UBA3

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 317-463

Epitope Target: UBA3

Epitope Species: human

Product Reactivity/Detection: UBA3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for UBA3 Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin