PCNA Antibody (PC10)recommended for detection of PCNA p36 protein expressed at high levels in proliferating cells of mouse, rat, human, insect and S. pombe origin by WB, IP, IF, IHC(P) and FCM

PCNA Antibody (PC10): sc-56

PCNA Antibody (PC10) is rated 4.9 out of 5 by 98.
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Datasheets
    • PCNA Antibody (PC10) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against rat PCNA made in the protein A expression vector pR1T2T
    • PCNA Antibody (PC10) is recommended for detection of PCNA p36 protein expressed at high levels in proliferating cells of mouse, rat, human, insect and S. pombe origin by WB, IP, IF, IHC(P) and FCM
    • PCNA Antibody (PC10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA; and to either TRITC, PerCP, PerCP-Cy5.5 or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PCNA (PC10): sc-56.
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Can I use this mouse monoclonal antibody for immunohistochemical staining of swine(pig) tissue?

Asked by: xgtian
Thank you for your question. We have not tested this antibody on swine or pig tissue, however, we are aware of a few publications citing use of this antibody in pig samples. For example, PMID: 26389662.
Answered by: Technical Support
Date published: 2019-07-13

We are new to IHC. Thinking of using this antibody, PCNA Antibody: sc-56on our chicken samples. Wondering if there are any preferences on the conjugate for the secondary antibody, such as HRP, biotin etc. We don't need fluorescence

Asked by: SDas
Thank you for your question. This antibody has been previously cited for detection of PCNA in chicken samples. For IHC, you could use the unconjugated antibody, with an appropriate secondary antibody for detection, or the Biotin conjugated primary with an ABC Staining System, or the HRP conjugate for direct immunoperoxidase staining. Using the directly conjugated primary antibody can save you time and money on reagents. We do not have a preference for this particular antibody, and think you would be successful using either version of the antibody, depending on your preferences in your lab.
Answered by: Technical Support
Date published: 2019-06-22

PCNA Antibody (PC10) how much of 200ug/ml of this antibody I am going to get in one order?

Asked by: Anukana
Thank you for your question. This is provided as 200 ug in 1 mL.
Answered by: Technical Support
Date published: 2019-04-13

I'm wondering about the region of PCNA that is recognized by this antibody SC-56, PC10? Would an N-terminal Flag tag get in the way? Thank you.

Asked by: Galyah
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not been determined. However, some report that this clone (PC10) recognizes an epitope within the amino acid range of 111-125. In that case, we would not expect an N-terminal Flag tag to prevent this antibody from recognizing PCNA that contains this sequence.
Answered by: Technical Support
Date published: 2019-03-21

Sir, I want to get free sample of PCNA Antibody (PC10): sc-56. Please update me with the process to avail. Thank you.

Asked by: Pinky Kaur
Thank you for your question. Please contact your local distributor for more information on how to receive a free 10 ug sample of this monoclonal antibody
Answered by: Tech Service
Date published: 2019-03-13

Could you please suggest a suitable secondary antibody kit for this product ?

Asked by: Senthil
Thank you for your question. You can find our secondary reagents here: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Technical Support
Date published: 2019-02-23

Could you please suggest a secondary antibody kit for this product as I am going to do IHC (P) in rat testis?

Asked by: Senthil
Thank you for your question. You can find our secondary reagents here: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Technical Support
Date published: 2019-02-23

What is the recommended method for IHC or IF staining of mouse tissue with this antibody?Can you give me a detailed protocols?

Asked by: robb
Thank you for your inquiry. Detailed protocols for IHC and IF applications are available on our website. Please refer to the following page: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Tech Help
Date published: 2018-01-23
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Rated 5 out of 5 by from anti-PCNA antibody works for western blot We used the anti-PCNA antibody for western blotting of HeLa whole cell lysate and detected a band of the correct size.
Date published: 2019-07-29
Rated 5 out of 5 by from Nice single bend! As title, only a strong nice single bend on my blot.
Date published: 2019-04-23
Rated 5 out of 5 by from PCNA (pc10) antibody Worked very well for western blot. 1:1000 dilution of the antibody gives very strong and specific signal.
Date published: 2019-04-09
Rated 5 out of 5 by from Good Antibody for IHC Good signal on European seabass (Dicentrarchus labrax) tissues fixed in Bouin's solution. Diluition 1:50
Date published: 2019-01-16
Rated 4 out of 5 by from Buon funzionamento in immunofluorescenza We performed indirect immunofluorescence staining of formalin-fixed MC3T3-E1 mouse preosteoblast cell line. Some cells presented a clear signal of nuclear positivity while other cells presented a weaker signal with smears in the cytoplasm.
Date published: 2018-11-05
Rated 4 out of 5 by from Fine for western, not so much for IP Used at 1:500 dilution for western blot, strong bands. However, this antibody works poorly in immunoprecipitation setting.
Date published: 2018-08-01
Rated 5 out of 5 by from Great antibody I got very clear band at low concentration for WB.
Date published: 2018-07-27
Rated 5 out of 5 by from Great antibody for IHC We use 1:100 on adult mouse brain sections, it worked great.
Date published: 2018-07-19
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