PCNA Antibody (PC10) recommended for detection of PCNA p36 protein expressed at high levels in proliferating cells of mouse, rat, human, insect and S. pombe origin by WB, IP, IF, IHC(P) and FCM

PCNA Antibody (PC10): sc-56

PCNA Antibody (PC10) is rated 4.9 out of 5 by 94.
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Datasheets
    • PCNA Antibody (PC10) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against rat PCNA made in the protein A expression vector pR1T2T
    • recommended for detection of PCNA p36 protein expressed at high levels in proliferating cells of mouse, rat, human, insect and S. pombe origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA; and to either TRITC, PerCP, PerCP-Cy5.5 or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of PCNA (PC10): sc-56.
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What is the recommended method for IHC or IF staining of mouse tissue with this antibody?Can you give me a detailed protocols?

Asked by: robb
Thank you for your inquiry. Detailed protocols for IHC and IF applications are available on our website. Please refer to the following page: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Tech Help
Date published: 2018-01-23

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: jerojero
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody PCNA Antibody (PC10): sc-56 is currently available conjugated to biotin (sc-56 B), 200 µg/ml, for WB, IHC(P) and ELISA; and to either TRITC (sc-56 TRITC, 200 µg/ml), PerCP (sc-56 PerCP) or Alexa Fluor® 405 (sc-56 AF405), 100 tests in 2 ml, for IF, IHC(P) and FCM
Answered by: Tech Service 3
Date published: 2016-12-15

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2016-12-14

Could you please suggest a suitable secondary antibody kit for this product ?

Asked by: Senthil

Could you please suggest a secondary antibody kit for this product as I am going to do IHC (P) in rat testis?

Asked by: Senthil

Can I use this mouse monoclonal antibody for immunohistochemical staining of swine(pig) tissue?

Asked by: xgtian

PCNA Antibody (PC10) how much of 200ug/ml of this antibody I am going to get in one order?

Asked by: Anukana
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Rated 4 out of 5 by from Buon funzionamento in immunofluorescenza We performed indirect immunofluorescence staining of formalin-fixed MC3T3-E1 mouse preosteoblast cell line. Some cells presented a clear signal of nuclear positivity while other cells presented a weaker signal with smears in the cytoplasm.
Date published: 2018-11-05
Rated 4 out of 5 by from Fine for western, not so much for IP Used at 1:500 dilution for western blot, strong bands. However, this antibody works poorly in immunoprecipitation setting.
Date published: 2018-08-01
Rated 5 out of 5 by from Great antibody I got very clear band at low concentration for WB.
Date published: 2018-07-27
Rated 5 out of 5 by from Great antibody for IHC We use 1:100 on adult mouse brain sections, it worked great.
Date published: 2018-07-19
Rated 5 out of 5 by from Great for WB Strong and specific signal with no background in WB of protein isolated from myometrium and leiomyoma
Date published: 2018-07-06
Rated 4 out of 5 by from Very good antibody Antibody gives a nice nuclear staining on tissue sections
Date published: 2018-06-07
Rated 3 out of 5 by from decent fluorescent IHC I got a decent fluo IHC on mouse neonate retina frozen section using at 1:100.
Date published: 2018-05-25
Rated 5 out of 5 by from PCNA Very good monoclonal antibody. High specificity: No background. I have used it in WB on human cell lysates (20 ug total protein in 12%SDS PAGE). Dilution 1:500 was successfull.
Date published: 2018-05-10
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