Melanoma Marker Antibody (HMB45) recommended for detection of a neuraminidase sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes in junctional and blue nevus cells of mouse, rat and human origin by WB, IP, IF and IHC(P)

Melanoma Marker Antibody (HMB45): sc-59305

Datasheets
    • Melanoma Marker Antibody (HMB45) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against extract of pigmented melanoma metastases from lymph nodes of human origin
    • recommended for detection of a neuraminidase sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes in junctional and blue nevus cells of mouse, rat and human origin by WB, IP, IF and IHC(P); non cross-reactive with non-melanocytic cells
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of Melanoma Marker (HMB45): sc-59305.
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Can this mouse monoclonal Melanoma Marker Antibody (HMB45): sc-59305, be used for staining of mouse tissue?

Asked by: jerojero
Yes, this antibody is recommended for detection in mouse. The best way to stain mouse tissue with a mouse monoclonal, is to use a directly conjugated primary. This antibody is available conjugated to HRP (sc-59305 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-59305 PE), fluorescein (sc-59305 FITC), Alexa Fluor® 488 (sc-59305 AF488) or Alexa Fluor® 647 (sc-59305 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Technical Support 10
Date published: 2017-01-16
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Rated 5 out of 5 by from Strong Staining of Melanoma We received this as a sample. We got this to work on the first try! HIER using Tris buffer pH9.0 for 30 minutes and a concentration of 1:200 looks great! We ran human (melanoma) and normal mouse skin. Both look great although we only need it for human staining.
Date published: 2017-12-20
Rated 5 out of 5 by from good results Band clear obtained using this antibody at 1/1000 dilution
Date published: 2017-09-08
Rated 4 out of 5 by from Fonctionnel J'ai essayé cet anticorps en culture 2 D et ça marche bien
Date published: 2017-07-25
Rated 5 out of 5 by from Effect satisfaction I highly recommend use 5% BSA diluted it at 1:500 for WB, bands were clear
Date published: 2017-06-01
Rated 5 out of 5 by from pretty resutlt We get pretty results in WB assay, with a specific band. Our samples are mouse cell , the working dilution is 1: 300.
Date published: 2017-05-27
Rated 5 out of 5 by from very happy Melanoma Marker antibody for WB detection, gives clean band
Date published: 2017-05-20
Rated 5 out of 5 by from Strong band for WB Melanoma Marker (HMB45) targets the “gp100” protein. Great for Western blot (WB).
Date published: 2016-12-28
Rated 5 out of 5 by from Clean WB blot with single strong band in SK Clean WB blot with single strong band in SK-MEL-28 whole cell lysate. -SCBT QC
Date published: 2015-04-23
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