HSV-1 ICP8 Antibody (10A3): sc-53329

HSV-1 ICP8 Antibody (10A3) is a mouse monoclonal IgG1 kappa light chain antibody that detects the HSV1 ICP8 protein of Herpes simplex virus origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-HSV-1 ICP8 antibody (10A3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The herpes simplex virus (HSV), commonly known for causing cold sores, is a highly contagious pathogen that can establish latent infections in the host, leading to potential reactivation and disease. HSV-1 ICP8, the major single-strand DNA-binding protein encoded by HSV1, plays a crucial role in the viral life cycle by facilitating the assembly of single-stranded DNA into a homologous duplex plasmid, which is essential for viral replication. Additionally, HSV-1 ICP8 exhibits helix destabilizing activity, allowing promotion of the reverse reaction at higher concentrations, thereby influencing the dynamics of DNA replication. Understanding HSV-1 ICP8 function is vital, as this protein not only aids in viral replication but also serves as a target for therapeutic interventions aimed at controlling HSV infections.

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HSV-1 ICP8 Antibody (10A3) References:

1. Photoaffinity labeling of the herpes simplex virus type-1 single-strand DNA-binding protein (ICP8) with oligodeoxyribonucleotides.  |  White, EJ. and Boehmer, PE. 1999. Biochem Biophys Res Commun. 264: 493-7. PMID: 10529391
2. Equilibrium binding of single-stranded DNA with herpes simplex virus type I-coded single-stranded DNA-binding protein, ICP8.  |  Gourves, AS., et al. 2000. J Biol Chem. 275: 10864-9. PMID: 10753882
3. In vitro strand exchange promoted by the herpes simplex virus type-1 single strand DNA-binding protein (ICP8) and DNA helicase-primase.  |  Nimonkar, AV. and Boehmer, PE. 2002. J Biol Chem. 277: 15182-9. PMID: 11832483
4. The herpes simplex virus type-1 single-strand DNA-binding protein (ICP8) promotes strand invasion.  |  Nimonkar, AV. and Boehmer, PE. 2003. J Biol Chem. 278: 9678-82. PMID: 12645567
5. On the mechanism of strand assimilation by the herpes simplex virus type-1 single-strand DNA-binding protein (ICP8).  |  Nimonkar, AV. and Boehmer, PE. 2003. Nucleic Acids Res. 31: 5275-81. PMID: 12954763
6. Catalysis of strand exchange by the HSV-1 UL12 and ICP8 proteins: potent ICP8 recombinase activity is revealed upon resection of dsDNA substrate by nuclease.  |  Reuven, NB., et al. 2004. J Mol Biol. 342: 57-71. PMID: 15313607
7. Visualization of the annealing of complementary single-stranded DNA catalyzed by the herpes simplex virus type 1 ICP8 SSB/recombinase.  |  Makhov, AM. and Griffith, JD. 2006. J Mol Biol. 355: 911-22. PMID: 16343538
8. Establishment of a novel foreign gene delivery system combining an HSV amplicon with an attenuated replication-competent virus, HSV-1 HF10.  |  Zhang, L., et al. 2006. J Virol Methods. 137: 177-83. PMID: 16854473
9. The herpes simplex virus type-1 single-strand DNA-binding protein, ICP8, increases the processivity of the UL9 protein DNA helicase.  |  Boehmer, PE. 1998. J Biol Chem. 273: 2676-83. PMID: 9446572

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HSV-1 ICP8 Antibody (10A3) has 22 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HSV-1 ICP8 Antibody (10A3) Specifications

Alternate Product Name: HSV-1 ICP8

Product Clone Name: 10A3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: HSV-1 ICP8

Epitope Species: viral

Product Reactivity/Detection: HSV1 ICP8

Species Reactivity/Detection: Herpes simplex virus

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for HSV-1 ICP8 Antibody (10A3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred HSV-1 ICP8 antibody is HSV-1 ICP8 Antibody (11E2): sc-53330.