Date published: 2025-11-4
Visit Santa Cruz Animal Health

1-800-457-3801

SCBT Portrait Logo
Seach Input
Contact Us
Sign In Account
Quick Order
Cart (0)
western blotting image from recommended Estrogen Receptor alpha (F-10) monoclonal antibody replacement. Image 369721
This polyclonal antibody has been discontinued. See our recommended Estrogen Receptor alpha (F-10) monoclonal antibody (sample data shown).
western blotting image from recommended Estrogen Receptor alpha (F-10) monoclonal antibody replacement. Image 369721
This polyclonal antibody has been discontinued. See our recommended Estrogen Receptor alpha (F-10) monoclonal antibody (sample data shown).
This polyclonal antibody has been discontinued. See our recommended Estrogen Receptor alpha (F-10) monoclonal antibody (sample data shown).

ERα Antibody (MC-20): sc-542

4.8(20)
Write a reviewAsk a question

  • ERα Antibody (MC-20) is a rabbit polyclonal IgG; 100 µg/ml
  • epitope mapping at the C-terminus of ERα of mouse origin
  • Discontinued polyclonal antibody

SEE ALSO...

Additional Estrogen Receptor Antibodies including Estrogen Receptor alpha, ER β, ERR α, ERR β and ERR γ

Ordering Information

Estrogen Receptor alpha (F-10): sc-8002 [ Recommended monoclonal replacement for ERα (MC-20) ]

Product NameCatalog #UNITPriceQtyFAVORITES

Estrogen Receptor alpha Antibody (F-10)

sc-8002
200 µg/ml
$316.00

Estrogen Receptor alpha Antibody (F-10): m-IgG Fc BP-HRP Bundle

sc-528195
200 µg Ab; 10 µg BP
$354.00

Estrogen Receptor alpha Antibody (F-10): m-IgG2a BP-HRP Bundle

sc-547044
200 µg Ab; 10 µg BP
$354.00

Estrogen Receptor alpha Antibody (F-10): m-IgGκ BP-HRP Bundle

sc-520517
200 µg Ab, 40 µg BP
$354.00

Estrogen Receptor alpha Antibody (F-10) X

sc-8002 X
200 µg/0.1 ml
$316.00

Estrogen Receptor alpha Antibody (F-10) AC

sc-8002 AC
500 µg/ml, 25% agarose
$416.00

Estrogen Receptor alpha Antibody (F-10) Alexa Fluor® 546

sc-8002 AF546
200 µg/ml
$357.00

Estrogen Receptor alpha Antibody (F-10) Alexa Fluor® 594

sc-8002 AF594
200 µg/ml
$357.00

Estrogen Receptor alpha Antibody (F-10) Alexa Fluor® 680

sc-8002 AF680
200 µg/ml
$357.00

Estrogen Receptor alpha Antibody (F-10) Alexa Fluor® 790

sc-8002 AF790
200 µg/ml
$357.00

Estrogen Receptor alpha (F-10) Neutralizing Peptide

sc-8002 P
100 µg/0.5 ml
$68.00

sc-8002 is not in the mouse tissues, I tried so hard with different antigen retrieval conditions, for both paraffin and cryosections on the mouse mammary gland. Really headache that SC542 is not available anymore.

Asked by: slimpopo
Thank you for your question. The ERα Antibody (F-10): sc-8002 is specific for an epitope mapping between amino acids 576-595 at the C-terminus of ERα of human origin and it is recommended for detection of ERα of human origin by WB, IP, IF, IHC(P) and ELISA. We do not recommend this antibody for detection of its epitope in murine samples. You can find alternative antibodies specific against the murine epitope by following this link: https://www.scbt.com/scbt/browse/er-alpha-Antibodies/_/N-1qbf0ke. For further information please contact our technical service directly.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-24

Hi Santa Cruz Biotechnology representative, I have an ERalpha (D-4) sc-514910 that I bought in 2016. I don't see it listed on the website and I was wondering if there is a reason for this.

Asked by: NotTom
Thank you for your inquiry. sc-514910 is polyclonal antibody and all the polyclonal antibodies have been discontinued since 2016. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2018-08-14

Dear Santa Cruz, Why was the very good antibody against the ER-alpha receptor SC-542 discontinued ?? Look at the reference list how many studies were published with this antibody. I also worked with the antibody (cf. Kruijver et al., 2002 JCN). Sincerely

Asked by: Kruijver
Thank you for your question. All our polyclonal antibodies have been discontinued, we are now monoclonal specific. We offer many excellent ER-alpha specific monoclonal antibodies that are fine alternatives to sc-542, such as sc-8002, sc-787 and sc-8005. You can find them here: https://www.scbt.com/scbt/browse/estrogen-receptor-Antibodies/_/N-cuom06
Answered by: Tech Service
Date published: 2017-11-21

Hi, I am wondering if you guys still have the old stock of sc-542 antibody that used to be 200ug/ml? I find that that one works better for my experiments. Please let me know whether I can purchase the old stock. Thank you! Yichen

Asked by: Yichen
Thank you for your question. We have completely switched over all of our stock of this rabbit polyclonal, ERalpha Antibody (MC-20): sc-542 to the new concentration of 100 µg/ml. The difference in antibody performance you are experiencing may be more related to the lot number of the product, rather than the concentration. This is a rabbit polyclonal antibody and new lots are purified from different host animals. As a result, it is typical to observe variation between different lots. And each new lot must be optimized. Please note that sc-542 is going to be discontinued at the end of this year. We recommend you try one of our suggested replacement mouse monoclonal antibodies against ERalpha. Please contact our Technical Service Department and we can send you a free sample to test of the best antibody for your research needs. Please call us at (800) 457-3801 option 2, email scbt@scbt.com, or connect with us on live chat.
Answered by: Technical Support
Date published: 2016-10-05
  • y_2025, m_10, d_14, h_6CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_en_US, sid_542, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 101ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Work in IHCWorked well in Immunohistochemistry with a dillution of 1/500
Date published: 2022-07-07
Rated 5 out of 5 by from well in WBwo use it for years,and test it in some cell and tissues.for this result,wo test it in human cell
Date published: 2016-12-05
Rated 4 out of 5 by from goodworks well in frozen section of mammary gland, fixation with 4% PFA 2h
Date published: 2016-11-30
Rated 3 out of 5 by from not very good in ChIPit did not work well in ChIP using mouse thymocyte cells
Date published: 2016-11-29
Rated 5 out of 5 by from Great for immunohistochemistryWorked very well in immunohistochemistry (1:100) on murine mammary gland tissue (paraffin embedded with antigen retrieval)
Date published: 2016-11-22
Rated 5 out of 5 by from Antibody was used on mouse pancreas for immunoblotAntibody was used on mouse pancreas for immunoblot at 1:200 giving excellent result.
Date published: 2016-02-02
Rated 5 out of 5 by from The antibody effect is very clearThe antibody effect is very clear, the background is low, with special perfect!
Date published: 2015-10-14
Rated 5 out of 5 by from Able to publish using the antibody for WBAble to publish using the antibody for WB and IHC in mouse sample.
Date published: 2015-09-16
  • y_2025, m_10, d_14, h_6
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_19
  • loc_en_US, sid_542, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT
ERα Antibody (MC-20) is rated 4.8 out of 5 by 20.
  • y_2025, m_10, d_14, h_6
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_19
  • loc_en_US, sid_542, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 98ms
  • REVIEWS, PRODUCT
1-800-457-3801
Visit Santa Cruz Animal Health|Visit San Juan Ranch
Santa Cruz Animal Health is a leading provider of animal health care products. ©2025 Santa Cruz Biotechnology Inc. All Rights Reserved.
Toll-Free: (800) 457-3801 - Terms & Conditions | Privacy Policy | Legal
“Santa Cruz Animal Health”, “San Juan Ranch”, the San Juan Ranch logo, “UltraCruz”, “Supplement of Champions”, are registered trademarks of Santa Cruz Biotechnology, Inc. All other trademarks are the property of their respective owners.