ERalpha Antibody (HC-20)
  • ERα Antibody (HC-20) is a rabbit polyclonal IgG provided at 100 µg/ml (Note concentration change)
  • epitope mapping at the C-terminus of ERα of human origin
  • recommended for detection of ERα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
  • available as TransCruz reagent for Gel Supershift and ChIP applications (sc-543 X, 100 µg/0.1 ml)
  • available as blocking peptide, sc-543 P

ERα Antibody (HC-20): sc-543

ERalpha Antibody (HC-20) is rated 4.588235294117647 out of 5 by 17.
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Datasheets
    • ERα Antibody (HC-20) is a rabbit polyclonal IgG provided at 100 µg/ml (Note concentration change)
    • epitope mapping at the C-terminus of ERα of human origin
    • recommended for detection of ERα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
    • available as TransCruz reagent for Gel Supershift and ChIP applications (sc-543 X, 100 µg/0.1 ml)
    • available as blocking peptide, sc-543 P
Monos, satisfaction guaranteed

ERα (HC-20) will be discontinued December 31, 2016; to receive a 10 µg sample of ERα (C-3): sc-514857, our highly recommended and much superior monoclonal antibody alternative to this product, please contact Technical Service.

Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
ERα Antibody (HC-20) sc-543 100 µg/ml $279.00
ERα Antibody (HC-20) X sc-543 X 100 µg/0.1 ml $279.00
ERα (HC-20) P sc-543 P 100 µg/0.5 ml $61.00

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ERalpha Antibody (HC-20) Product Citations

See how others have used ERalpha Antibody (HC-20). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 230 total

PMID: # 26763249
Vareslija, D. et al. 2016. Adaptation to AI therapy in breast cancer can induce dynamic alterations in ER activity resulting in estrogen independent metastatic tumours. Clinical cancer research : an official journal of the American Association for Cancer Research.

PMID: # 26137275
He, DE. et al. 2015. Clinicopathological characteristics and prognosis of breast cancer patients with type 2 diabetes mellitus. Molecular and clinical oncology. 3: 607-612.

PMID: # 26348925
Pesiri, V. et al. 2015. Estrogen Receptor α L429 and A430 Regulate 17β-estradiol-induced cell proliferation via CREB1. Cellular signalling.

PMID: # 25860340
Totta, P. et al. 2015. Clathrin heavy chain interacts with estrogen receptor α and modulates 17β-estradiol signaling. Molecular endocrinology (Baltimore, Md.). me20141385.

PMID: # 26480297
Totta, P. et al. 2015. Modulation of 17β-estradiol signaling on cellular proliferation by caveolin-2. Journal of cellular physiology.

PMID: # 26079946
Ma, S. et al. 2015. Tyrosine dephosphorylation enhances the therapeutic target activity of epidermal growth factor receptor (EGFR) by disrupting its interaction with estrogen receptor (ER). Oncotarget. 6: 13320-33.

PMID: # 25582774
Patki, M. et al. 2015. Differential effects of estrogen-dependent transactivation vs. transrepression by the estrogen receptor on invasiveness of HER2 overexpressing breast cancer cells. Biochemical and biophysical research communications. 457: 404-11.

PMID: # 26080448
Gao, WW. et al. 2015. Arginine methylation of HSP70 regulates retinoid acid-mediated RARβ2 gene activation. Proceedings of the National Academy of Sciences of the United States of America. 112: E3327-36.

PMID: # 24736371
Totta, P. et al. 2014. Lysosomal Function Is Involved in 17b-Estradiol-Induced Estrogen Receptor a Degradation and Cell Proliferation Plos One. 9: e94880.

PMID: # 25242450
Ronghe, A. et al. 2014. Differential regulation of estrogen receptors α and β by 4-(E)-{(4-hydroxyphenylimino)-methylbenzene,1,2-diol}, a novel resveratrol analog. The Journal of steroid biochemistry and molecular biology. 144 Pt B: 500-12.

Citations 1 to 10 of 230 total
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Rated 1 out of 5 by from Used for rat sample Used for rat sample, obtained very faint band at expected size, but many non-specific bands that could not account for. Not able to obtain positive result with positive control. Trying a mouse monoclonal to see if improves specificity.
Date published: 2015-04-23
Rated 4 out of 5 by from This is the best antibody against ER This is the best antibody against ER-alpha that I have so far used. I used this antibody for detection of ER-alpha during different stages of breast cancer models. I chose the antibody because of its high specificity.
Date published: 2015-02-12
Rated 5 out of 5 by from I have used this antibody to do CHIP assay I have used this antibody to do CHIP assay as many paper used. To be honest, it works quite well. What s more, it is much cheaper than the similar products of other company.
Date published: 2015-10-15
Rated 5 out of 5 by from this is the best anti this is the best anti-ERalpha I ever used. It works very very well for IP, IF WB, CHIP CELLS MCF-7, T47D-1 APPLICATION IP, IF, WB, CHIP
Date published: 2015-09-11
Rated 5 out of 5 by from I have been using this product for the past I have been using this product for the past week against ER alpha in mouse samples and am pleased with the results so far.
Date published: 2015-03-31
Rated 4 out of 5 by from WB data was really good WB data was really good. We are using is IF and the images are also turning out really good.
Date published: 2015-09-04
Rated 4 out of 5 by from Gets results for IF in methanol Gets results for IF in methanol-fixed T-47D cells showing nuclear localization -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from I used this in WB in mouse cells and works great I used this in WB in mouse cells and works great, correct band seen at 1:500 dilution.
Date published: 2015-10-07
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