ER alpha Antibody (HC-20) DISCONTINUED: recommended for detection of ERα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

ERα Antibody (HC-20): sc-543

ERα Antibody (HC-20) is rated 4.5 out of 5 by 22.
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    • ERα Antibody (HC-20) is a rabbit polyclonal IgG; 100 µg/ml
    • epitope mapping at the C-terminus of ERα of human origin
    • recommended for detection of ERα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
Monos, satisfaction guaranteed

ERα (HC-20) has been discontinued and replaced by ERα (F-10): sc-8002. This new monoclonal antibody is more specific, provides a stronger signal and eliminates lot to lot variation characteristic of most, if not all, polyclonal antibodies.

Ordering Information

ERα (F-10): sc-8002 [ Recommended monoclonal replacement for ERα (HC-20) ]
PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
ERα Antibody (F-10) sc-8002 200 µg/ml $284.00
ERα Antibody (F-10) AC sc-8002 AC 500 µg/ml, 25% agarose $372.00
ERα (F-10) Blocking Peptide sc-8002 P 100 µg/0.5 ml $62.00
ERα Antibody (F-10) X sc-8002 X 200 µg/0.1 ml $284.00

Hi I was using sc-543x for my chip experiments. I have few experiments left to do with the same antibody. why have been this antibody discontinued? Thanks

Asked by: prati
Thank you for your question. Yes, our rabbit polyclonal antibody, ERα (HC-20): sc-543, has been discontinued and is no longer available. Our highly recommended replacement monoclonal antibodies for ERα have also been validated for ChIP. Both clones, ERα (F-10): sc-8002 and ERα (D-12): sc-8005, have been cited in numerous publications for use in Chromatin Immunoprecipitation (ChIP) applications. Please contact our Technical Service Department if you would like to request a free 10 µg sample of these antibodies to test in your application. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2019-02-21
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Rated 5 out of 5 by from WB ER Wb for Estrogen receptor in MCF7 cells, 50ug of whole protein extract were used
Date published: 2019-02-13
Rated 4 out of 5 by from ER-alpha antibody western blot It is a good antibody for Er-alpha detection by Western blot . We used it in a 1:1000 dilution in human placenta samples and the results can be observed in this paper. It is an antibody of specific recognition.
Date published: 2018-05-17
Rated 3 out of 5 by from Used to detect ER alpha protein levels This antibody is very useful for detection of ER alpha protein levels in animal brain tissue. In particular, we used this antibody to detect protein levels in mytosol in hippocampus and prefrontal cortex of rats. In general, we are satisfied with the obtained results from our samples. Dilutions of Ab, up to 1:1000.
Date published: 2017-11-23
Rated 5 out of 5 by from Good for WB I tested this antibody for western blot using MCF7 total protein. As seen in photo, I got a specific band of ERalpha.
Date published: 2017-01-10
Rated 5 out of 5 by from Published WB and ChIP data on HMLE and MCF10A Published WB and ChIP data on HMLE and MCF10A cells and on MEF
Date published: 2016-06-24
Rated 5 out of 5 by from It is very clearn for western blot It is very clearn for western blot
Date published: 2015-10-20
Rated 5 out of 5 by from I have used this antibody to do CHIP assay I have used this antibody to do CHIP assay as many paper used. To be honest, it works quite well. What s more, it is much cheaper than the similar products of other company.
Date published: 2015-10-15
Rated 5 out of 5 by from I used this in WB in mouse cells and works great I used this in WB in mouse cells and works great, correct band seen at 1:500 dilution.
Date published: 2015-10-07
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