ER alpha Antibody (HC-20)
  • ERα Antibody (HC-20) is a rabbit polyclonal IgG; 100 µg/ml
  • epitope mapping at the C-terminus of ERα of human origin
  • recommended for detection of ERα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
  • TransCruz reagent for Gel Supershift and ChIP applications (sc-543 X, 100 µg/0.1 ml)
  • blocking peptide, sc-543 P

ERα Antibody (HC-20): sc-543

ERα Antibody (HC-20) is rated 4.6 out of 5 by 19.
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    • ERα Antibody (HC-20) is a rabbit polyclonal IgG; 100 µg/ml
    • epitope mapping at the C-terminus of ERα of human origin
    • recommended for detection of ERα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-543 X, 100 µg/0.1 ml)
    • blocking peptide, sc-543 P
Monos, satisfaction guaranteed

ERα (HC-20) has been discontinued; try ERα (F-10): sc-8002, our highly recommended monoclonal antibody alternative. If you have an interest in testing a 10 µg sample of the monoclonal alternative, please contact Technical Service.

Ordering Information

ERα (F-10): sc-8002 [ Recommended monoclonal replacement for ERα (HC-20) ]
PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
ERα Antibody (F-10) sc-8002 200 µg/ml $279.00
ERα Antibody (F-10) AC sc-8002 AC 500 µg/ml, 25% ag $366.00
ERα (F-10) Blocking Peptide sc-8002 P 100 µg/0.5 ml $61.00
ERα Antibody (F-10) X sc-8002 X 200 µg/0.1 ml $279.00

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ER alpha Antibody (HC-20) Product Citations

See how others have used ER alpha Antibody (HC-20). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 232 total

PMID: # 27009360
Totta, P. et al. 2016. Dynamin II is required for 17β-estradiol signaling and autophagy-based ERα degradation. Scientific reports. 6: 23727.

PMID: # 26987957
Mitrović, N. et al. 2016. 17β-Estradiol upregulates ecto-5'-nucleotidase (CD73) in hippocampal synaptosomes of female rats through action mediated by estrogen receptor-α and -β. Neuroscience. 324: 286-96.

PMID: # 26763249
Vareslija, D. et al. 2016. Adaptation to AI therapy in breast cancer can induce dynamic alterations in ER activity resulting in estrogen independent metastatic tumours. Clinical cancer research : an official journal of the American Association for Cancer Research.

PMID: # 26137275
He, DE. et al. 2015. Clinicopathological characteristics and prognosis of breast cancer patients with type 2 diabetes mellitus. Molecular and clinical oncology. 3: 607-612.

PMID: # 26348925
Pesiri, V. et al. 2015. Estrogen Receptor α L429 and A430 Regulate 17β-estradiol-induced cell proliferation via CREB1. Cellular signalling.

PMID: # 25860340
Totta, P. et al. 2015. Clathrin heavy chain interacts with estrogen receptor α and modulates 17β-estradiol signaling. Molecular endocrinology (Baltimore, Md.). me20141385.

PMID: # 26480297
Totta, P. et al. 2015. Modulation of 17β-estradiol signaling on cellular proliferation by caveolin-2. Journal of cellular physiology.

PMID: # 26079946
Ma, S. et al. 2015. Tyrosine dephosphorylation enhances the therapeutic target activity of epidermal growth factor receptor (EGFR) by disrupting its interaction with estrogen receptor (ER). Oncotarget. 6: 13320-33.

PMID: # 25582774
Patki, M. et al. 2015. Differential effects of estrogen-dependent transactivation vs. transrepression by the estrogen receptor on invasiveness of HER2 overexpressing breast cancer cells. Biochemical and biophysical research communications. 457: 404-11.

PMID: # 26080448
Gao, WW. et al. 2015. Arginine methylation of HSP70 regulates retinoid acid-mediated RARβ2 gene activation. Proceedings of the National Academy of Sciences of the United States of America. 112: E3327-36.

Citations 1 to 10 of 232 total

Hi I was using sc-543x for my chip experiments. I have few experiments left to do with the same antibody. why have been this antibody discontinued? Thanks

Asked by: prati
Thank you for your question. Yes, our rabbit polyclonal antibody, ERα (HC-20): sc-543, has been discontinued and is no longer available. Our highly recommended replacement monoclonal antibodies for ERα have also been validated for ChIP. Both clones, ERα (F-10): sc-8002 and ERα (D-12): sc-8005, have been cited in numerous publications for use in Chromatin Immunoprecipitation (ChIP) applications. Please contact our Technical Service Department if you would like to request a free 10 µg sample of these antibodies to test in your application. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-07-13
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Rated 5 out of 5 by from Good for WB I tested this antibody for western blot using MCF7 total protein. As seen in photo, I got a specific band of ERalpha.
Date published: 2017-01-10
Rated 5 out of 5 by from Published WB and ChIP data on HMLE and MCF10A Published WB and ChIP data on HMLE and MCF10A cells and on MEF
Date published: 2016-06-24
Rated 5 out of 5 by from It is very clearn for western blot It is very clearn for western blot
Date published: 2015-10-20
Rated 5 out of 5 by from I have used this antibody to do CHIP assay I have used this antibody to do CHIP assay as many paper used. To be honest, it works quite well. What s more, it is much cheaper than the similar products of other company.
Date published: 2015-10-15
Rated 5 out of 5 by from I used this in WB in mouse cells and works great I used this in WB in mouse cells and works great, correct band seen at 1:500 dilution.
Date published: 2015-10-07
Rated 5 out of 5 by from So far So far, so good!
Date published: 2015-10-01
Rated 5 out of 5 by from Worked very well in WB and IP Worked very well in WB and IP.
Date published: 2015-09-29
Rated 5 out of 5 by from this is the best anti this is the best anti-ERalpha I ever used. It works very very well for IP, IF WB, CHIP CELLS MCF-7, T47D-1 APPLICATION IP, IF, WB, CHIP
Date published: 2015-09-11
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