Cytokeratin 19 Antibody (A-3) recommended for detection of Cytokeratin 19 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Cytokeratin 19 Antibody (A-3): sc-376126

Cytokeratin 19 Antibody (A-3) is rated 4.2 out of 5 by 14.
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Datasheets
    • Cytokeratin 19 Antibody (A-3) is a mouse monoclonal IgG3 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 7-29 at the N-terminus of Cytokeratin 19 of human origin
    • recommended for detection of Cytokeratin 19 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
    • blocking peptide, sc-376126 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of Cytokeratin 19 (A-3): sc-376126.
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How can Cytokeratin 19 Antibody (A-3): sc-376126 be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody is currently available conjugated to phycoerythrin (sc-376126 PE), fluorescein (sc-376126 FITC), Alexa Fluor® 488 (sc-376126 AF488) or Alexa Fluor® 647 (sc-376126 AF647)
Answered by: Technical Support 14
Date published: 2017-01-09
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Rated 1 out of 5 by from Did not work in rat tongue Keratin19 labels taste bud in rat tongue but this one is staining only few cell in the taste bud with a high background.
Date published: 2018-01-19
Rated 4 out of 5 by from work fine in IHC,but unknown in IF The antibody work in IF and IHC at 1:100 for mouse primary liver cancer cells, hepa1-6 cells and 3T3 cells, but the FITC is negative through stained.Maybe these cells essentially can't express the protein or other reasons. After we searched the certain cell lines of full CK19 expression,namely hepaG2, BUT we are sorry to be note that the sample is over. (The photo of IHC work fine, but we didn't upload resulted by the store location of photos.) 3T3 cells for negative group.
Date published: 2017-10-24
Rated 5 out of 5 by from Very good for FFPE This antibody was used on formalin-fixed paraffin-embedded human carcinomas at a dilution of 1:30 for 15 minutes. Staining was very good.
Date published: 2017-08-10
Rated 1 out of 5 by from Doesn't work for immunofluorescence on mouse cells Used on mouse ovarian epithelial cells at 1/100 overnight at 4C then 1/500 goat α-mouse secondary for 1H at room temp. Low signal to noise ratio.
Date published: 2017-03-24
Rated 4 out of 5 by from Cytokeratin 19 We used Cytokeratin 19 as a marker of HepG2 cells with IF and got nice stainning
Date published: 2017-02-16
Rated 5 out of 5 by from Outstanding for multiple applications !! Great antibody for WB, IHC and IF studies; much better than polyclonal alternatives
Date published: 2017-01-11
Rated 5 out of 5 by from Versatile Antibody This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, equine, and human samples.
Date published: 2016-12-29
Rated 4 out of 5 by from Good product. I recieved it 1 mouth ago and used it for IF,1:100,not bad.
Date published: 2016-12-22
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