Date published: 2025-9-9

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V-ATPase D1 Anticorpo (34-Z): sc-81887

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Fichas de dados
  • V-ATPase D1 Anticorpo 34-Zé um anticorpo monoclonal produzido em camundongo IgG1 κ, citado em 7 publicações, fornecido em 100 µg/ml
  • Produzido contra recombinante V-ATPase D1 de human origem
  • recomendado para a detecção de V-ATPase D1 of human origin by WB, IP, IF, IHC(P) and ELISA
  • Atualmente, ainda não concluímos a identificação do(s) reagente(s) de deteção secundário(s) preferido(s) para V-ATPase D1 Anticorpo (34-Z). Este trabalho está em curso.

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VEJA TAMBÉM

A H+-ATPase de tipo vacuolar (V-ATPase) é uma enzima com várias subunidades responsável pela acidificação dos organelos intracelulares eucarióticos. As V-ATPases bombeiam protões contra um gradiente eletroquímico, enquanto as F-ATPases invertem o processo, sintetizando assim ATP. Um domínio V1 periférico, responsável pela hidrólise do ATP, e um domínio V0 integral, responsável pela translocação de protões, constituem o complexo V-ATPase. Nove subunidades (A-H) constituem o domínio V1 e cinco subunidades (A, D, C, C' e C") constituem o domínio V0. A V-ATPase D1 (ATPase, H+ transporting, lysosomal, V0 subunit D1), também conhecida como ATP6V0D1, P39, VATX, VMA6, ATP6D ou VPATPD, é a subunidade D do domínio V0. Expressa de forma ubíqua, a V-ATPase D1 actua em conjunto com outras subunidades V0 para acidificar cataliticamente uma variedade de compartimentos intracelulares, sintetizando assim ATP para ser utilizado no transporte vacuolar.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

V-ATPase D1 Anticorpo (34-Z)

sc-81887
100 µg/ml
$333.00

I detected only 100kDa band and weak band under 37kDa in HUVECs using this antibody. That's why I am asking about the transfected data in the data sheets.  Can this antibody detect other component of V-ATP ase?

Asked by: Maxi
Thank you for your question. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting this antibody for Western blotting.
Answered by: Tech Service
Date published: 2019-11-06

The molecular weight of V-ATPase D1 is 40kDa and the WB data of Hela in the data sheet is 40kDa. But WB of human V-ATPase D1 transfected 293T cells in the data sheet is around 100kDa. Which is the true molecular weight?

Asked by: Maxi
Thank you for your question. Thank you for bringing this discrepancy to our attention. The observed molecular weight in the transfected lysate is much higher than expected. We will be reviewing this data for accuracy and will remove or replace it if necessary.
Answered by: Technical Support
Date published: 2019-11-06

Can sc-81887: V-ATPase D1 (34-Z) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Professor Griffin
Thank you for your question. Yes, sc-81887: V-ATPase D1 (34-Z) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good specificity observed in WB in HeLa wholeGood specificity observed in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-26
Rated 4 out of 5 by from Produced robust immunoperoxidase cytoplasmicProduced robust immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human stomach tissue. -SCBT QC
Date published: 2015-03-31
Rated 4 out of 5 by from Published data for WB in HeLa cellsPublished data for WB in HeLa cells. -SCBT Publication Review
Date published: 2015-03-28
Rated 4 out of 5 by from Good antibody for WB in human VGood antibody for WB in human V-ATPase D1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-03-13
Rated 4 out of 5 by from Published data for WB in epithelial tissuesPublished data for WB in epithelial tissues from human breast cancer tissue. -SCBT Publication Review
Date published: 2015-01-12
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V-ATPase D1 Anticorpo (34-Z) is rated 4.0 out of 5 by 5.
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