SREBP-1 Antibody (F-10): sc-365514

SREBP-1 Antibody (F-10) is a mouse monoclonal IgG3 kappa light chain antibody that detects SREBP-1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SREBP-1 Antibody (F-10) is available in a non-conjugated form, allowing for versatile use in research settings. SREBP-1, also known as ADD1 (adipocyte determination and differentiation factor), plays a crucial role in lipid metabolism by regulating the expression of genes involved in cholesterol and fatty acid synthesis. SREBP-1 is synthesized as a precursor that is anchored to the nuclear envelope and endoplasmic reticulum. Upon depletion of cellular sterol levels, SREBP-1 precursor undergoes proteolytic cleavage, releasing a soluble NH2-terminal fragment that translocates to the nucleus to activate transcription of target genes, including the low-density lipoprotein (LDL) receptor. The LDL receptor is essential for the endocytic uptake of cholesterol-carrying lipoproteins, thereby maintaining cellular and plasma cholesterol homeostasis. Notably, SREBP-1 activity is tightly regulated by sterol levels, as excess sterols inhibit SREBP-1 cleavage, highlighting SREBP-1′s importance in cellular lipid regulation and potential implications in metabolic disorders.

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SREBP-1 Antibody (F-10) References:

1. PAS kinase drives lipogenesis through SREBP-1 maturation.  |  Wu, X., et al. 2014. Cell Rep. 8: 242-55. PMID: 25001282
2. Co-regulation of SREBP-1 and mTOR ameliorates lipid accumulation in kidney of diabetic mice.  |  Wang, H., et al. 2015. Exp Cell Res. 336: 76-84. PMID: 26112216
3. Feedback Loop Regulation of SCAP/SREBP-1 by miR-29 Modulates EGFR Signaling-Driven Glioblastoma Growth.  |  Ru, P., et al. 2016. Cell Rep. 16: 1527-1535. PMID: 27477273
4. An important role of SREBP-1 in HBV and HCV co-replication inhibition by PTEN.  |  Wu, Q., et al. 2018. Virology. 520: 94-102. PMID: 29803738
5. SREBP-1 inhibitor Betulin enhances the antitumor effect of Sorafenib on hepatocellular carcinoma via restricting cellular glycolytic activity.  |  Yin, F., et al. 2019. Cell Death Dis. 10: 672. PMID: 31511501
6. Inhibition of SREBP-1 Activation by a Novel Small-Molecule Inhibitor Enhances the Sensitivity of Hepatocellular Carcinoma Tissue to Radiofrequency Ablation.  |  Zou, XZ., et al. 2021. Front Oncol. 11: 796152. PMID: 34900747
7. A Novel Small-Molecule Inhibitor of SREBP-1 Based on Natural Product Monomers Upregulates the Sensitivity of Lung Squamous Cell Carcinoma Cells to Antitumor Drugs.  |  Ma, DB., et al. 2022. Front Pharmacol. 13: 895744. PMID: 35662712
8. Bufalin Inhibits Tumorigenesis and SREBP-1-Mediated Lipogenesis in Hepatocellular Carcinoma via Modulating the ATP1A1/CA2 Axis.  |  Huang, CJ., et al. 2023. Am J Chin Med. 51: 461-485. PMID: 36655687
9. Exosomes from adipose-derived stem cells activate sebocytes through the PI3K/AKT/SREBP-1 pathway to accelerate wound healing.  |  Zhang, Y., et al. 2024. Cell Tissue Res. 396: 329-342. PMID: 38411945
10. Polyphyllin I induces rapid ferroptosis in acute myeloid leukemia through simultaneous targeting PI3K/SREBP-1/SCD1 axis and triggering of lipid peroxidation.  |  Zhou, X., et al. 2024. J Nat Med. 78: 618-632. PMID: 38668832

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SREBP-1 Antibody (F-10) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-SREBP-1 Antibody (F-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SREBP-1 Antibody (F-10) Specifications

Alternate Product Name: SREBF1

Product Clone Name: F-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 1119-1147

Epitope Target: SREBP-1

Epitope Species: human

Product Reactivity/Detection: SREBP-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for SREBP-1 Antibody (F-10): P: Blocking Peptide

The preferred SREBP-1 antibody is SREBP-1 Antibody (2A4): sc-13551.