SLD5 Antibody (D-7): sc-398784

SLD5 Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects SLD5 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SLD5 (D-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SLD5 protein, also known as GINS4 (GINS complex subunit 4), is a 223 amino acid protein located in both cytoplasm and nucleus, and plays a pivotal role in DNA replication initiation and progression. As a critical component of GINS complex, which consists of Psf1, Psf2, and Psf3 proteins, SLD5 is essential for proper assembly of this heterotetrameric complex. GINS complex is highly conserved across evolution and crucial for maintaining genomic stability during cell division. Notably, SLD5 has been found significantly up-regulated in aggressive melanomas, highlighting potential role in cancer biology. Interaction between SLD5 and Psf1 is particularly important, as Psf1 binds to single-stranded DNA, facilitating GINS complex′s function in DNA replication. Using anti-SLD5 antibody (D-7), researchers can gain valuable insights into DNA replication mechanisms and SLD5′s implications in various cellular processes and diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SLD5 Antibody (D-7) References:

1. PSF1 is essential for early embryogenesis in mice.  |  Ueno, M., et al. 2005. Mol Cell Biol. 25: 10528-32. PMID: 16287864
2. Identification and characterization of mouse PSF1-binding protein, SLD5.  |  Kong, L., et al. 2006. Biochem Biophys Res Commun. 339: 1204-7. PMID: 16338220
3. Structure of the human GINS complex and its assembly and functional interface in replication initiation.  |  Kamada, K., et al. 2007. Nat Struct Mol Biol. 14: 388-96. PMID: 17417653
4. Molecular architecture of the human GINS complex.  |  Boskovic, J., et al. 2007. EMBO Rep. 8: 678-84. PMID: 17557111
5. Comprehensive expression profiling of tumor cell lines identifies molecular signatures of melanoma progression.  |  Ryu, B., et al. 2007. PLoS One. 2: e594. PMID: 17611626
6. Crystal structure of the GINS complex and functional insights into its role in DNA replication.  |  Chang, YP., et al. 2007. Proc Natl Acad Sci U S A. 104: 12685-90. PMID: 17652513
7. The initiation step of eukaryotic DNA replication.  |  Pospiech, H., et al. 2010. Subcell Biochem. 50: 79-104. PMID: 20012578
8. PSF3 marks malignant colon cancer and has a role in cancer cell proliferation.  |  Nagahama, Y., et al. 2010. Biochem Biophys Res Commun. 392: 150-4. PMID: 20059967
9. Structure and function of the GINS complex, a key component of the eukaryotic replisome.  |  MacNeill, SA. 2010. Biochem J. 425: 489-500. PMID: 20070258

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SLD5 Antibody (D-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-SLD5 Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SLD5 Antibody (D-7) Specifications

Alternate Product Name: SLD5

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-223

Epitope Target: SLD5

Epitope Species: human

Product Reactivity/Detection: SLD5

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for SLD5 Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin