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western blotting image from recommended Shh (E-1) monoclonal antibody replacement. Image 137799 — Este anticorpo policlonal foi descontinuado. Consulta o nosso anticorpo monoclonal Shh (E-1) recomendado (dados de amostra apresentados).

Este anticorpo policlonal foi descontinuado. Consulta o nosso anticorpo monoclonal Shh (E-1) recomendado (dados de amostra apresentados).

Shh Anticorpo (H-160): sc-9024

4.5(13)

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Shh Anticorpo H-160 é um anticorpo policlonal produzido em coelho IgG fornecido em200 µg/ml
Anticorpo policlonal descontinuado

Veja citações de produtos (56)

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Descricao

VEJA TAMBÉM

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor^® é uma marca comercial da Molecular Probes Inc., OR., EUA

Informacoes sobre ordens

Shh (E-1): sc-365112 [ anticorpo monoclonal substituto recomendado para Shh (H-160) ]

Nome do Produto	Numero de Catalogo	UNID	Preco	Qde	FAVORITOS
Shh Anticorpo (E-1)	sc-365112	200 µg/ml	$322.00
Shh Anticorpo (E-1) AC	sc-365112 AC	500 µg/ml, 25% agarose	$424.00
Shh Anticorpo (E-1) HRP	sc-365112 HRP	200 µg/ml	$322.00
Shh Anticorpo (E-1) FITC	sc-365112 FITC	200 µg/ml	$336.00
Shh Anticorpo (E-1) PE	sc-365112 PE	200 µg/ml	$349.00
Shh Anticorpo (E-1) Alexa Fluor^® 488	sc-365112 AF488	200 µg/ml	$364.00
Shh Anticorpo (E-1) Alexa Fluor^® 546	sc-365112 AF546	200 µg/ml	$364.00
Shh Anticorpo (E-1) Alexa Fluor^® 594	sc-365112 AF594	200 µg/ml	$364.00
Shh Anticorpo (E-1) Alexa Fluor^® 647	sc-365112 AF647	200 µg/ml	$364.00
Shh Anticorpo (E-1) Alexa Fluor^® 680	sc-365112 AF680	200 µg/ml	$364.00
Shh Anticorpo (E-1) Alexa Fluor^® 790	sc-365112 AF790	200 µg/ml	$364.00
Shh (E-1) peptídeo neutralizante	sc-365112 P	100 µg/0.5 ml	$69.00

Would this (discontinued) polyclonal antibody be expected to detect Indian Hedgehog (Ihh)?  The description stated it detected "broad range of hedgehog family proteins of mouse, rat, human.."

Asked by: David F

Thank you for your question. Yes, this antigen shares significant sequence identity (>90%) with Ihh.

Answered by: Technical Support

Date published: 2019-05-17

Hi, one comment - this H-160 pAb is useful for mouse tissue IF or IHC. Your mAb would be less useful for the same purpose. Where could I find the antigen region information that was used to raise the H-160 pAb?

Asked by: kurakura

This now discontinued rabbit polyclonal antibody, Shh (H-160): sc-9024, was raised against a recombinant protein corresponding to amino acids 41-200 of Shh of human origin. Our recommended replacement mouse monoclonal antibody, Shh (E-1): sc-365112, is also suitable for IF and IHC(P) on mouse tissue. We offer the antibody directly conjugated to HRP, or a variety of fluorescent dyes and you could use the conjugated antibody for staining without having to probe using any anti-mouse IgG secondary antibody for detection. There are also methods for mouse on mouse staining using the unconjugated primary antibody. Please contact Technical Service if you have further questions.

Answered by: Technical Support

Date published: 2018-12-21

Rated 5 out of 5 by IvanaS from Shh Antibody (H-160) in WBThis antibody worked perfectly in WB both for human cervical cancer cell lines and immortalized human keratinocytes (HaCaT). It has shown differential expression among different cell lines and the expression was in line with the results of semi-quantitative RT-PCR.

Date published: 2021-04-14

Rated 5 out of 5 by RIAZ from sc-9024I found the sc-9024 antibody very useful for immunohistochemistry, western blotting and immuno fluorescence confocal microscopy experiments. Thank you

Date published: 2019-10-03

Rated 5 out of 5 by Audrey_H_ from Works well for Western blot and IHCWorks well for Western blot and IHC-P

Date published: 2015-12-18

Rated 5 out of 5 by Audrey_H_ from Works well for IHC on FFPE human pancreaticWorks well for IHC on FFPE human pancreatic adenocarcinoma sections.

Date published: 2015-12-14

Rated 4 out of 5 by Anon from Published data using antibody with paraffinPublished data using antibody with paraffin embedded sections.

Date published: 2015-11-12

Rated 4 out of 5 by TS from Various publishable data in IHC using mouse embryoVarious publishable data in IHC using mouse embryo, bladder epithelial, ovary, testes and duodena. -SCBT Publication Review

Date published: 2015-04-14

Rated 5 out of 5 by MS from Produced excellent immunoperoxidase cytoplasmicProduced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human stomach tissue. -SCBT QC

Date published: 2015-03-30

Rated 5 out of 5 by Amanda from Great AntibodyGreat Antibody! Do not have any complaints as it has been continuously performing well.

Date published: 2015-03-30

Shh Anticorpo (H-160) Reviews - page 2

Shh Anticorpo (H-160) is rated 4.5 out of 5 by 13.

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Shh Anticorpo (H-160): sc-9024

Shh Anticorpo (E-1)

Shh Anticorpo (E-1) AC

Shh Anticorpo (E-1) HRP

Shh Anticorpo (E-1) FITC

Shh Anticorpo (E-1) PE

Shh Anticorpo (E-1) Alexa Fluor® 488

Shh Anticorpo (E-1) Alexa Fluor® 546

Shh Anticorpo (E-1) Alexa Fluor® 594

Shh Anticorpo (E-1) Alexa Fluor® 647

Shh Anticorpo (E-1) Alexa Fluor® 680

Shh Anticorpo (E-1) Alexa Fluor® 790

Shh (E-1) peptídeo neutralizante

Would this (discontinued) polyclonal antibody be expected to detect Indian Hedgehog (Ihh)? &nbsp;The description stated it detected &quot;broad range of hedgehog family proteins of mouse, rat, human..&quot;

Hi, one comment - this H-160 pAb is useful for mouse tissue IF or IHC. Your mAb would be less useful for the same purpose. Where could I find the antigen region information that was used to raise the H-160 pAb?

Shh Anticorpo (E-1) Alexa Fluor^® 488

Shh Anticorpo (E-1) Alexa Fluor^® 546

Shh Anticorpo (E-1) Alexa Fluor^® 594

Shh Anticorpo (E-1) Alexa Fluor^® 647

Shh Anticorpo (E-1) Alexa Fluor^® 680

Shh Anticorpo (E-1) Alexa Fluor^® 790

Would this (discontinued) polyclonal antibody be expected to detect Indian Hedgehog (Ihh)? The description stated it detected "broad range of hedgehog family proteins of mouse, rat, human.."