Shh Antibody (E-1): sc-365112

Shh Antibody (E-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Shh protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Shh antibody (E-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Sonic hedgehog (Shh) protein plays a crucial role in embryonic development and tissue patterning, particularly in the central nervous system and limb formation. Shh functions as a signaling molecule that mediates cell-cell interactions, influencing growth and differentiation of neighboring cells. Shh is processed into an amino-terminal peptide that remains associated with the cell surface and a carboxy-terminal glycosylated peptide that is more diffusible, allowing for longer-distance signaling. This dual processing establishes essential signaling gradients vital for proper developmental processes. Shh interaction with specific receptors, such as Patched and Smoothened, further underscores Shh importance in developmental biology and implications in various diseases, including cancer.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Shh Antibody (E-1) References:

1. Sonic Hedgehog Signaling and Tooth Development.  |  Hosoya, A., et al. 2020. Int J Mol Sci. 21: PMID: 32111038
2. Germline Elongator mutations in Sonic Hedgehog medulloblastoma.  |  Waszak, SM., et al. 2020. Nature. 580: 396-401. PMID: 32296180
3. METTL3 regulates m6A methylation of PTCH1 and GLI2 in Sonic hedgehog signaling to promote tumor progression in SHH-medulloblastoma.  |  Zhang, ZW., et al. 2022. Cell Rep. 41: 111530. PMID: 36288719
4. Sonic hedgehog signaling in craniofacial development.  |  Xu, J., et al. 2023. Differentiation. 133: 60-76. PMID: 37481904
5. The long and short of hedgehog signaling.  |  Johnson, RL. and Tabin, C. 1995. Cell. 81: 313-6. PMID: 7736584
6. Floor plate and motor neuron induction by different concentrations of the amino-terminal cleavage product of sonic hedgehog autoproteolysis.  |  Roelink, H., et al. 1995. Cell. 81: 445-55. PMID: 7736596
7. Long-range sclerotome induction by sonic hedgehog: direct role of the amino-terminal cleavage product and modulation by the cyclic AMP signaling pathway.  |  Fan, CM., et al. 1995. Cell. 81: 457-65. PMID: 7736597
8. Requirement of 19K form of Sonic hedgehog for induction of distinct ventral cell types in CNS explants.  |  Martí, E., et al. 1995. Nature. 375: 322-5. PMID: 7753196
9. Sonic hedgehog induces the differentiation of ventral forebrain neurons: a common signal for ventral patterning within the neural tube.  |  Ericson, J., et al. 1995. Cell. 81: 747-56. PMID: 7774016
10. Function of protein kinase A in hedgehog signal transduction and Drosophila imaginal disc development.  |  Li, W., et al. 1995. Cell. 80: 553-62. PMID: 7867063
11. Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity.  |  Echelard, Y., et al. 1993. Cell. 75: 1417-30. PMID: 7916661
12. Sonic hedgehog mediates the polarizing activity of the ZPA.  |  Riddle, RD., et al. 1993. Cell. 75: 1401-16. PMID: 8269518

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Shh Antibody (E-1) has 53 citations in a variety of scientific publications.

Primary antibodies like Anti-Shh Antibody (E-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Shh Antibody (E-1) Specifications

Alternate Product Name: sonic hedgehog

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 25-57

Epitope Target: Shh

Epitope Species: human

Product Reactivity/Detection: Ihh and Shh

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, porcine and avian

Additional Non-Mammalian Reactivity: avian

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 53 citations in various scientific publications

Available Conjugates/Forms for Shh Antibody (E-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin