pyridoxal kinase Antibody (F-12): sc-390082

Pyridoxal kinase Antibody (F-12) is a mouse monoclonal IgG1 antibody that detects pyridoxal kinase in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Pyridoxal kinase plays a crucial role in the phosphorylation of vitamin B6, a vital step in converting it to pyridoxal 5′ phosphate (PLP), the active form of vitamin B6 that serves as a coenzyme in numerous enzymatic reactions essential for maintaining metabolic homeostasis. This 312-amino acid cytoplasmic protein functions as a homodimer and is expressed ubiquitously across various tissues, with three identified isoforms, including isoform 3, which is specifically expressed in adult testis and spermatozoa. Pyridoxal kinase exhibits optimal activity at a pH range of 5.5 to 6.0, highlighting functional importance in slightly acidic cellular environments. The gene encoding pyridoxal kinase, PDXK, is located on chromosome 21q22.3 and has been implicated as a potential candidate gene for autoimmune polyglandular disease type 1, a genetic disorder associated with this chromosomal region. Anti-pyridoxal kinase antibody (F-12) is an invaluable tool for researchers studying vitamin B6 metabolism and its implications in health and disease.

The preferred pyridoxal kinase antibody is pyridoxal kinase Antibody (E-2): sc-365173.