PML Antibody (E-11): sc-377390

PML Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects PML protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PML Antibody (E-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PML protein, a crucial zinc finger transcription factor, plays a significant role in regulating gene expression and cellular differentiation, particularly in myeloid cells. PML expression is vital for maintaining normal hematopoiesis, and disruptions in PML function, such as those caused by the t(15;17) chromosomal translocation, can lead to acute promyelocytic leukemia. This translocation results in the formation of the PML/RARα chimeric protein, which interferes with normal myeloid differentiation by recruiting the nuclear co-repressor-histone deacetylase complex, ultimately promoting an undifferentiated state and increased cell proliferation. PML protein′s ability to interact with various transcriptional regulators and chromatin-modifying enzymes underscores PML′s importance in both normal cellular processes and the pathogenesis of leukemia, making anti-PML antibody (E-11) an essential tool for researchers studying these critical biological pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PML Antibody (E-11) has 26 citations in a variety of scientific publications.

Primary antibodies like Anti-PML Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PML Antibody (E-11) Specifications

Alternate Product Name: PML

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 27-63

Epitope Target: PML

Epitope Species: human

Product Reactivity/Detection: all isoforms of PML

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 26 citations in various scientific publications

Available Conjugates/Forms for PML Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred PML antibody is PML Antibody (PG-M3): sc-966.