PCNA Antibody (PC10): sc-56

PCNA Antibody (PC10) is a mouse monoclonal IgG2a kappa light chain antibody. Proliferating Cell Nuclear Antigen (PCNA) is a critical nuclear protein involved in DNA replication and repair in eukaryotic cells. PCNA forms a ring structure that acts as a scaffold for recruiting proteins, supporting DNA polymerases during replication. PCNA plays key roles in DNA metabolism, chromatin remodeling, cell cycle regulation, and DNA repair pathways, ensuring high-fidelity DNA replication. PCNA is regulated by post-translational modifications, allowing dynamic response to DNA damage and maintenance of genomic integrity. Dysregulation of PCNA is linked to cancer, making PCNA an important biomarker for prognosis and a potential therapeutic target. PCNA monoclonal antibody (PC10) is used in various conjugated forms for IF and ELISA, providing clear nuclear staining during DNA replication. PCNA (PC10) antibody′s high specificity and sensitivity make this product useful for studying cell proliferation, DNA repair, and tumor growth, aiding in the development of therapeutic strategies for diseases related to abnormal cell proliferation.

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PCNA Antibody (PC10) References:

1. The contribution of E2F-regulated transcription to Drosophila PCNA gene function.  |  Thacker, SA., et al. 2003. Curr Biol. 13: 53-8. PMID: 12526745
2. Germline mutations at microsatellite loci in homozygous and heterozygous mutants for mismatch repair and PCNA genes in Drosophila.  |  Baida, A., et al. 2003. DNA Repair (Amst). 2: 827-33. PMID: 12826283
3. The human proliferating Cell nuclear antigen regulates transcriptional coactivator p300 activity and promotes transcriptional repression.  |  Hong, R. and Chakravarti, D. 2003. J Biol Chem. 278: 44505-13. PMID: 12937166
4. Armadillo/Pangolin regulates PCNA and DREF promoter activities.  |  Kwon, E., et al. 2004. Biochim Biophys Acta. 1679: 256-62. PMID: 15358517
5. GFP-PCNA as an S-phase marker in embryos during the first and subsequent cell cycles.  |  Kisielewska, J., et al. 2005. Biol Cell. 97: 221-9. PMID: 15584900
6. Germline genomic instability in PCNA mutants of Drosophila: DNA fingerprinting and microsatellite analysis.  |  López, A., et al. 2005. Mutat Res. 570: 253-65. PMID: 15708584
7. The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S+G2+M phase fraction (flow cytometric analysis) and prognosis.  |  Woods, AL., et al. 1991. Histopathology. 19: 21-7. PMID: 1680784
8. Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.  |  Waseem, NH. and Lane, DP. 1990. J Cell Sci. 96 (Pt 1): 121-9. PMID: 1695635
9. The archaeal PCNA proteins.  |  Pan, M., et al. 2011. Biochem Soc Trans. 39: 20-4. PMID: 21265741
10. Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites.  |  Bravo, R. and Macdonald-Bravo, H. 1987. J Cell Biol. 105: 1549-54. PMID: 2889739
11. Identification of PCNA-interacting protein motifs in human DNA polymerase δ.  |  Khandagale, P., et al. 2020. Biosci Rep. 40: PMID: 32314787
12. PCNA as Protein-Based Nanoruler for Sub-10 nm Fluorescence Imaging.  |  Helmerich, DA., et al. 2024. Adv Mater. 36: e2310104. PMID: 38009560

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PCNA Antibody (PC10) has 2,548 citations in a variety of scientific publications.

Primary antibodies like Anti-PCNA Antibody (PC10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PCNA Antibody (PC10) Specifications

Alternate Product Name: PCNA

Product Clone Name: PC10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 111-125

Epitope Target: PCNA

Epitope Species: rat

Product Reactivity/Detection: PCNA p36 protein expressed at high levels in proliferating cells

Species Reactivity/Detection: mouse, rat, human, insect, S. pombe

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2,548 citations in various scientific publications

Available Conjugates/Forms for PCNA Antibody (PC10): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine