Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NUB1 CRISPR/Cas9 KO Plasmid (m): sc-424677
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NUB1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NUB1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NUB1 Antibody (F-10): sc-377003
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NUB1 CRISPR/Cas9 KO Plasmid (m) | sc-424677 | 20 µg | $397.00 |
Overview
Nub1 (NEDD8 ultimate buster 1) encodes NUB1, an interferon-inducible protein that binds the ubiquitin-like modifier NEDD8 and promotes proteasome-dependent clearance of NEDD8-conjugated substrates. By regulating neddylation dynamics and turnover of proteins linked to cullin-RING E3 ligases, NUB1 influences ubiquitin–proteasome system homeostasis, cell-cycle progression, and cellular stress responses. NUB1 also interacts with ubiquitin-like proteins such as FAT10, connecting innate immune signaling to regulated protein degradation. Dysregulation of these pathways is frequently implicated in proteostasis imbalance and inflammatory signaling phenotypes, making Nub1 a useful node for mechanistic studies in mouse models.
NUB1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nub1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nub1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nub1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NUB1 protein expression.
This CRISPR knockout system enables efficient generation of Nub1-deficient cell models for investigation of NUB1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nub1 exon(s) critical for NUB1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nub1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NUB1 CRISPR/Cas9 KO Plasmid (m) and NUB1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nub1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NUB1 HDR Plasmid (m) and NUB1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nub1 homology arms to support homology-directed repair at defined Nub1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.