Date published: 2025-9-7

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Notch1 Anticorpo (A-8): sc-376403

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  • Notch1 Anticorpo A-8é um anticorpo monoclonal produzido em camundongo IgM κ Notch1 anticorpo, citado em 50 publicações, fornecido em 200 µg/ml
  • específico para um epítopo mapeado entre os aminoácidos 2425-2444 no C-terminus de Notch 1 de human origem
  • recomendado para a detecção de Notch 1 de mouse, rat e human origem em
  • disponível conjugado à agarose/b> para IP; HRP/b> para WB, IHC(P) e ELISA; ficoeritrina/b>, FITC/b>, Alexa Fluor® 488/b> ou Alexa Flúor® 647/b> para IF, IHC(P) e FCM
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de Notch1 (A-8): sc-376403.
  • O m-IgGκ BP-HRP é o reagente de deteção secundário preferido para o anticorpo Notch1 (A-8) para aplicações WB e IHC(P). Este reagente é agora oferecido num pacote com o anticorpo Notch1 (A-8)(ver informações de encomenda abaixo). Para mais conjugados m-IgGκ BP, ver a nossa lista completa de Proteínas de Ligação IgG de Rato.

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VEJA TAMBÉM

O Anticorpo Notch 1 (A-8) é um anticorpo monoclonal Notch 1 IgM κ de camundongo (também designado anticorpo NOTCH1) que detecta a proteína Notch 1 de origem de camundongo, rato e humana por WB, IP, IF, IHC(P) e ELISA. O anticorpo Notch 1 (A-8) está disponível na forma de anticorpo anti-Notch 1 não conjugado, bem como em várias formas conjugadas de anticorpo anti-Notch 1, incluindo agarose, HRP, PE, FITC e vários conjugados Alexa Fluor®. Acredita-se que os receptores transmembrana LIN-12/Notch desempenhem um papel central no desenvolvimento, regulando as decisões sobre o destino das células. Quatro homólogos de Notch (Notch 1, Notch 2, Notch 3 e Notch 4) foram identificados em mamíferos. Os genes Notch são expressos em uma variedade de tecidos embrionários e adultos, sugerindo que os genes estão envolvidos em várias vias de sinalização. Descobriu-se que as proteínas Notch são superexpressas ou rearranjadas em tumores humanos. Os ligantes para Notch incluem Jagged1, Jagged2 e Delta. O Jagged1 pode ativar o Notch e impedir a diferenciação de mioblastos ao inibir a expressão de genes estruturais e reguladores musculares. O Jagged2 pode estar envolvido no desenvolvimento de tecidos que dependem de interações epiteliais e mesenquimais. Além de sua expressão normal na glândula adrenal e na placenta, a expressão de Delta também foi encontrada em tumores neuroendócrinos.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

Notch1 Anticorpo (A-8)

sc-376403
200 µg/ml
$316.00

Pacote do Notch1 (A-8): m-IgGκ BP-HRP

sc-552043
200 µg Ab; 40 µg BP
$354.00

Notch1 Anticorpo (A-8) AC

sc-376403 AC
500 µg/ml, 25% agarose
$416.00

Notch1 Anticorpo (A-8) Alexa Fluor® 488

sc-376403 AF488
200 µg/ml
$357.00

Notch1 Anticorpo (A-8) Alexa Fluor® 647

sc-376403 AF647
200 µg/ml
$357.00

Notch1 Anticorpo (A-8) FITC

sc-376403 FITC
200 µg/ml
$330.00

Notch1 Anticorpo (A-8) HRP

sc-376403 HRP
200 µg/ml
$316.00

Notch1 (A-8) peptídeo neutralizante

sc-376403 P
100 µg/0.5 ml
$68.00

Notch1 Anticorpo (A-8) PE

sc-376403 PE
200 µg/ml
$343.00

Hello, What secondary antibody should i use for WB? I can't see anywhere the host species,..Thanks

Asked by: myrto
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-04-20

Hello, I would like to use Notch-1 Antibody (A-8) primarily for western blot and later maybe for IC. Could you let me know if I could purchase the antibody conjugated to Alexa Fluor 488 and use it for WB Too or for WB only the HRP conjugated works?

Asked by: myrto
Yes, you can use conjugates to Alexa Fluor 488 for Western blotting.
Answered by: Tech Sales
Date published: 2021-03-15

Can I use this antibody for IF-P staining? If not which product can I use for IF tissue staining?

Asked by: JordanA
Thank you for your question. This antibody is recommended for IF and IHC(P), or immunofluorescence and immunohistochemistry on formalin-fixed paraffin-embedded tissue sections.
Answered by: Technical Support
Date published: 2019-07-18

I purchased this Notch 1 antibody (A-8) to do western blot for human breast cancer cell lines lysates, but I am not sure where the band will appear in the gel (110KD or 210 KD?). Please tell me a relatively accurate position. THX~

Asked by: Tianzi
Thank you for your question. The expected molecular weight for Notch one is ~300 kDa (precursor) and two possible cleaved forms which run around ~180 and ~115-120kDa.
Answered by: Tech Service
Date published: 2021-04-19

Hi, I am intersted in Notch 1 Antibody (A-8) Alexa Fluor® 647 (sc-376403 AF647, 314USD) I just saw its concentration is 200ug/mL. How about the volume? Thanks.

Asked by: Bin Zhao
Thank you for your question. It is provided as 1ml.
Answered by: Tech Service
Date published: 2018-11-21

I would like to use Notch 1 Antibody (A-8): sc-376403 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-376403 is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-26
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Rated 5 out of 5 by from Notch1 worked very well in IHCNotch1 worked very well in IHC in human tissues by DAB staining
Date published: 2023-05-16
Rated 4 out of 5 by from Buon anticorpo per WB su campioni proteici umanitestato per western blot ad una concentrazione di 1:1000 su estratti proteici totali di cellule THP1 e su LX2 umane. tempo di esposizione rapido, banda netta
Date published: 2022-11-21
Rated 1 out of 5 by from No staining in murine heartThe antibody has been tested with different antigen retrival protocols. None of them worked out.
Date published: 2022-03-03
Rated 4 out of 5 by from Good for flow cytometryThis antibody works well for flow cytometry at 1:50 but requires permeabilization of the cell membrane.
Date published: 2019-02-26
Rated 4 out of 5 by from THIS IS A GOOD ANTIBODYI got a free sample of this antibody and it is working very fine. I am interested in buying a new vial.
Date published: 2018-03-29
Rated 4 out of 5 by from Antibody works greatBought this Notch1 monoclonal A8 Ab. Works better than Notch C-20 antibody. Specificity is good
Date published: 2018-02-08
Rated 3 out of 5 by from be patient !This antibodies is working just fine but for me I just need to wait and give it a little long exposure time..
Date published: 2017-12-21
Rated 2 out of 5 by from backgroundthe antibody was tested on rat tissue samples in western blot. A signal is detected but with a high background level
Date published: 2017-12-13
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Notch1 Anticorpo (A-8) is rated 4.3 out of 5 by 27.
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