MMAB Antibody (G-3): sc-271424
- MMAB Antibody (G-3) is a mouse monoclonal IgG1 κ MMAB antibody provided at 200 µg/ml
- raised against amino acids 34-250 mapping at the C-terminus of MMAB of human origin
- MMAB Antibody (G-3) is recommended for detection of MMAB of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-MMAB Antibody (G-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MMAB Antibody (G-3) for WB applications. These reagents are now offered in bundles with MMAB Antibody (G-3) (see ordering information below).
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MMAB Antibody (G-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects MMAB protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MMAB (G-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MMAB protein, also known as methylmalonic aciduria (cobalamin deficiency) type B protein or Cob(I)alamin adenosyltransferase, is a crucial mitochondrial protein predominantly expressed in skeletal muscle and liver tissues. MMAB protein is integral to the biosynthesis of adenosylcobalamin (AdoCbl), which serves as an essential cofactor for the enzyme methylmalonyl-CoA mutase (MUT). MMAB protein catalyzes the final step of the AdoCbl biosynthesis pathway, converting vitamin B12 into AdoCbl. AdoCbl is vital for the catabolism of various metabolites, including cholesterol, branched-chain amino acids, and odd-numbered fatty acids. Deficiencies or mutations in the MMAB gene can lead to methylmalonic aciduria type B, a condition characterized by impaired AdoCbl synthesis, highlighting MMAB protein′s critical function in metabolic processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MMAB Antibody (G-3) References:
- Functional genomic, biochemical, and genetic characterization of the Salmonella pduO gene, an ATP:cob(I)alamin adenosyltransferase gene. | Johnson, CL., et al. 2001. J Bacteriol. 183: 1577-84. PMID: 11160088
- Identification of the gene responsible for the cblB complementation group of vitamin B12-dependent methylmalonic aciduria. | Dobson, CM., et al. 2002. Hum Mol Genet. 11: 3361-9. PMID: 12471062
- The structural basis for methylmalonic aciduria. The crystal structure of archaeal ATP:cobalamin adenosyltransferase. | Saridakis, V., et al. 2004. J Biol Chem. 279: 23646-53. PMID: 15044458
- Mutation and biochemical analysis of patients belonging to the cblB complementation class of vitamin B12-dependent methylmalonic aciduria. | Lerner-Ellis, JP., et al. 2006. Mol Genet Metab. 87: 219-25. PMID: 16410054
- Impact of cblB mutations on the function of ATP:cob(I)alamin adenosyltransferase in disorders of vitamin B12 metabolism. | Zhang, J., et al. 2006. Mol Genet Metab. 87: 315-22. PMID: 16439175
- Regulation by SREBP-2 defines a potential link between isoprenoid and adenosylcobalamin metabolism. | Murphy, C., et al. 2007. Biochem Biophys Res Commun. 355: 359-64. PMID: 17300749
- Novel mutations found in two genes of thai patients with isolated methylmalonic acidemia. | Keeratichamroen, S., et al. 2007. Biochem Genet. 45: 421-30. PMID: 17410422
- Restricted role for methionine synthase reductase defined by subcellular localization. | Froese, DS., et al. 2008. Mol Genet Metab. 94: 68-77. PMID: 18221906
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MMAB Antibody (G-3) | sc-271424 | 200 µg/ml | $322.00 | |||
MMAB Antibody (G-3): m-IgG Fc BP-HRP Bundle | sc-529183 | 200 µg Ab; 10 µg BP | $361.00 | |||
MMAB Antibody (G-3): m-IgGκ BP-HRP Bundle | sc-521942 | 200 µg Ab, 40 µg BP | $361.00 | |||
MMAB Antibody (G-3) AC | sc-271424 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MMAB Antibody (G-3) HRP | sc-271424 HRP | 200 µg/ml | $322.00 | |||
MMAB Antibody (G-3) FITC | sc-271424 FITC | 200 µg/ml | $336.00 | |||
MMAB Antibody (G-3) PE | sc-271424 PE | 200 µg/ml | $349.00 | |||
MMAB Antibody (G-3) Alexa Fluor® 488 | sc-271424 AF488 | 200 µg/ml | $364.00 | |||
MMAB Antibody (G-3) Alexa Fluor® 546 | sc-271424 AF546 | 200 µg/ml | $364.00 | |||
MMAB Antibody (G-3) Alexa Fluor® 594 | sc-271424 AF594 | 200 µg/ml | $364.00 | |||
MMAB Antibody (G-3) Alexa Fluor® 647 | sc-271424 AF647 | 200 µg/ml | $364.00 | |||
MMAB Antibody (G-3) Alexa Fluor® 680 | sc-271424 AF680 | 200 µg/ml | $364.00 | |||
MMAB Antibody (G-3) Alexa Fluor® 790 | sc-271424 AF790 | 200 µg/ml | $364.00 |