Date published: 2025-9-9

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MAD1 Anticorpo (D-6): sc-137026

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Fichas de dados
  • MAD1 Anticorpo D-6é um anticorpo monoclonal produzido em camundongo IgG2b κ, citado em 1 publicações, fornecido em 200 µg/ml
  • suscitadas contra os aminoácidos 241-540 que se mapeiam numa região interna de MAD1 de origem mouse
  • recomendado para a detecção de MAD1 de mouse, rat e human origem em
  • O m-IgGκ BP-HRP é o reagente de deteção secundário preferido para o anticorpo MAD1 (D-6) para aplicações WB. Este reagente é agora oferecido num pacote com o anticorpo MAD1 (D-6)(ver informações de encomenda abaixo). Para mais conjugados m-IgGκ BP, ver a nossa lista completa de Proteínas de Ligação IgG de Rato.

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VEJA TAMBÉM

Cell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD1 and MAD2 (for mitotic arrest-deficient 1 and 2) are components of the mitotic spindle checkpoint. Incorrect spindle assembly in normal cells leads to mitotic arrest. MAD1 prevents the onset of anaphase until all chromosomes are aligned correctly at the metaphase plate and is crucial for anchoring MAD2L1 to the nuclear periphery. It also plays an important role in septum positioning. MAD1 can form a homo-dimer, but may also form a heterodimer with MAD2 to form the tetrameric MAD1L1-MAD2L1 core complex. MAD1 localizes primarily to the nucleus, but during mitosis, it moves from a nuclear distribution to the centrosome, to the spindle midzone and then on to the midbody. MAD1 activity is induced by BUB1 and the protein is hyperphosphorylated after mitotic spindle damage and/or in late S through M phase. Defects in the gene encoding for MAD1, MAD1L1, play a major role in the development and progression of various cancer types.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

MAD1 Anticorpo (D-6)

sc-137026
200 µg/ml
$316.00

Pacote do MAD1 (D-6): m-IgGκ BP-HRP

sc-534642
200 µg Ab; 40 µg BP
$354.00

What is the recommended starting dilution for Western blot for MAD1 (D-6): sc-137026 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-137026.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Nice Western blot data of MAD1 expression in nonNice Western blot data of MAD1 expression in non-transfected and mouse MAD1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-03-15
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MAD1 Anticorpo (D-6) is rated 5.0 out of 5 by 1.
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