LEF-1 Antibody (B-6): sc-374522

LEF-1 Antibody (B-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects LEF-1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-LEF-1 antibody (B-6) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. LEF-1, a member of the TCF (T cell factor) family of transcription factors, plays a crucial role in regulating gene expression during development, particularly in lymphoid and neuronal cells. LEF-1 is characterized by a high mobility group (HMG) box, which enables DNA-binding capability. This structural feature allows LEF-1 to interact with β-catenin in the nucleus, forming complexes that activate Wnt target genes, which are vital for various cellular processes including cell proliferation and differentiation. Dysregulation of this pathway, often due to mutations in the adenomatous polyposis coli (APC) gene, can lead to β-catenin accumulation and aberrant activation of LEF-1 target genes, contributing to oncogenesis, particularly in colorectal cancer. LEF-1 (B-6) antibody′s availability in multiple formats enhances experimental versatility, making this tool essential for researchers studying Wnt signaling and its implications in development and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LEF-1 Antibody (B-6) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-LEF-1 Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LEF-1 Antibody (B-6) Specifications

Alternate Product Name: LEF-1

Product Clone Name: B-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 61-130

Epitope Target: LEF-1

Epitope Species: human

Product Reactivity/Detection: LEF-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for LEF-1 Antibody (B-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred LEF-1 antibody is LEF-1 Antibody (B-10): sc-374412.