Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Histone Deacetylase 2 (HDAC2) CRISPR/Cas9 KO Plasmid (r): sc-437286
- Target species: rat
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Histone Deacetylase 2 (HDAC2) CRISPR/Cas9 Knockout (KO) Plasmid (r) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Histone Deacetylase 2 (HDAC2) genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Histone Deacetylase 2 Antibody (C-8): sc-9959
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Histone Deacetylase 2 (HDAC2) CRISPR/Cas9 KO Plasmid (r) | sc-437286 | 20 µg | $397.00 |
Overview
Histone deacetylase 2 (HDAC2) is a class I Zn²⁺-dependent histone deacetylase that removes acetyl groups from lysine residues on histones and other nuclear proteins, promoting chromatin compaction and transcriptional repression. In rat cells, HDAC2 functions within corepressor assemblies such as Sin3, NuRD, and CoREST to coordinate gene-expression programs controlling cell-cycle progression, differentiation, synaptic plasticity, and DNA damage responses. Through crosstalk with signaling networks including MAPK/ERK, PI3K-AKT, and p53-dependent checkpoints, HDAC2 contributes to context-specific regulation of proliferation and stress adaptation. Dysregulated HDAC2 activity has been associated with altered inflammatory gene expression and epigenetic remodeling relevant to neurobiology and oncogenic transformation, making it a frequent target in mechanistic studies of chromatin-driven phenotypes.
Histone Deacetylase 2 (HDAC2) CRISPR/Cas9 KO Plasmid (r) is a pool of plasmids designed for targeted disruption of the gene in rat cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Histone Deacetylase 2 (HDAC2) protein expression.
This CRISPR knockout system enables efficient generation of -deficient cell models for investigation of Histone Deacetylase 2 (HDAC2) signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting exon(s) critical for Histone Deacetylase 2 (HDAC2) function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Histone Deacetylase 2 (HDAC2) CRISPR/Cas9 KO Plasmid (r) and Histone Deacetylase 2 (HDAC2) CRISPR/Cas9 KO Plasmid (r2) target distinct sites within the locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Histone Deacetylase 2 (HDAC2) HDR Plasmid (r) and Histone Deacetylase 2 (HDAC2) HDR Plasmid (r2) contain a puromycin resistance cassette and an RFP reporter flanked by homology arms to support homology-directed repair at defined target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.