Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GPR14 Double Nickase Plasmid (h): sc-402393-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR14 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPR14 Double Nickase Plasmid (h) and GPR14 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UTS2R. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GPR14 Antibody (D-4): sc-514460
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR14 Double Nickase Plasmid (h) | sc-402393-NIC | 20 µg | $410.00 | |||
GPR14 Double Nickase Plasmid (h2) | sc-402393-NIC-2 | 20 µg | $410.00 |
UTS2R encodes the G protein-coupled receptor GPR14, a high-affinity receptor for the neuropeptide urotensin II that regulates vascular tone, smooth muscle contraction, and neuroendocrine signaling. Upon ligand engagement, GPR14 couples predominantly to Gαq/11 to activate phospholipase C, intracellular Ca²⁺ mobilization, and downstream MAPK/ERK signaling, with additional effects on RhoA/ROCK-dependent cytoskeletal remodeling. These pathways link UTS2R to modulation of endothelial and cardiomyocyte function, inflammatory signaling, and cellular proliferation programs. Altered urotensin II–UTS2R signaling has been associated with cardiovascular and metabolic disease biology, supporting mechanistic studies in relevant human cell models.
GPR14 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UTS2R locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UTS2R. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UTS2R function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UTS2R-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.