GADD 45 alpha Antibody (C-6): sc-48394
- GADD 45α Antibody (C-6) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-165 representing full length GADD 45α of human origin
- recommended for detection of GADD 45α of human origin by WB, IP, IF and ELISA
- See GADD 45α (C-4): sc-6850 for GADD 45α antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GADD 45α Antibody (C-6) for WB applications. These reagents are now offered in bundles with GADD 45α Antibody (C-6) (see ordering information below).
GADD 45α Antibody (C-6) is a mouse monoclonal IgG1 antibody that detects GADD 45α in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GADD 45α plays a crucial role in the cellular response to DNA damage, acting as a mediator between the p53 tumor suppressor pathway and DNA repair mechanisms. GADD 45α is primarily located in the nucleus, where anti-GADD 45α antibody (C-6) can detect interactions with key proteins involved in DNA repair processes, such as cyclin-dependent kinases (Cdks) and proliferating cell nuclear antigen (PCNA). Through binding to these proteins, GADD 45α not only inhibits their activity, thereby preventing cell cycle progression in the presence of DNA damage, but also enhances the efficiency of DNA excision repair. This function is essential for maintaining genomic stability and preventing the propagation of damaged DNA, which can lead to tumorigenesis. GADD 45α monoclonal antibody (C-6) serves as an invaluable tool for researchers studying the intricate mechanisms of cell cycle regulation and DNA repair pathways in human cells.
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LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GADD 45 alpha Antibody (C-6) References:
- ATR and GADD45alpha mediate HIV-1 Vpr-induced apoptosis. | Andersen, JL., et al. 2005. Cell Death Differ. 12: 326-34. PMID: 15650754
- DNA mismatch repair-dependent activation of c-Abl/p73alpha/GADD45alpha-mediated apoptosis. | Li, LS., et al. 2008. J Biol Chem. 283: 21394-403. PMID: 18480060
- Induction of GADD45α protects M17 neuroblastoma cells against MPP*. | Wang, XF., et al. 2014. IUBMB Life. 66: 786-92. PMID: 25469469
- Upregulation of GADD45α in light-damaged retinal pigment epithelial cells. | Gao, ML., et al. 2016. Cell Death Discov. 2: 16013. PMID: 27551507
- GADD45α is involved in the apoptosis of lymphocytes induced by riboflavin and ultraviolet light. | Yang, P., et al. 2017. Transfusion. 57: 646-656. PMID: 27905125
- GADD45α sensitizes cervical cancer cells to radiotherapy via increasing cytoplasmic APE1 level. | Li, Q., et al. 2018. Cell Death Dis. 9: 524. PMID: 29743554
- Transcriptional repression of IKKβ by p53 in arsenite-induced GADD45α accumulation and apoptosis. | Hu, Y., et al. 2019. Oncogene. 38: 731-746. PMID: 30177839
- The Natural Compound Neobractatin Induces Cell Cycle Arrest by Regulating E2F1 and Gadd45α. | Zheng, Z., et al. 2019. Front Oncol. 9: 654. PMID: 31380287
- EGR1/GADD45α Activation by ROS of Non-Thermal Plasma Mediates Cell Death in Thyroid Carcinoma. | Jung, SN., et al. 2021. Cancers (Basel). 13: PMID: 33477921
- KP372-1-Induced AKT Hyperactivation Blocks DNA Repair to Synergize With PARP Inhibitor Rucaparib via Inhibiting FOXO3a/GADD45α Pathway. | Jiang, L., et al. 2022. Front Oncol. 12: 976292. PMID: 36203459
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GADD 45α Antibody (C-6) | sc-48394 | 200 µg/ml | $322.00 | |||
GADD 45α Antibody (C-6): m-IgG Fc BP-HRP Bundle | sc-536827 | 200 µg Ab; 10 µg BP | $361.00 | |||
GADD 45α Antibody (C-6): m-IgGκ BP-HRP Bundle | sc-533994 | 200 µg Ab; 40 µg BP | $361.00 | |||
GADD 45α Antibody (C-6): m-IgG1 BP-HRP Bundle | sc-544845 | 200 µg Ab; 20 µg BP | $361.00 |