Chibby Antibody (C-10): sc-393295
- Chibby Antibody (C-10) is a mouse monoclonal IgG2b κ Chibby antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 16-37 within an internal region of Chibby of human origin
- Chibby Antibody (C-10) is recommended for detection of Chibby of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Chibby Antibody (C-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Chibby Antibody (C-10). This work is in progress.
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Chibby Antibody (C-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects Chibby protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Chibby antibody (C-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Chibby protein, also known as Cytosolic leucine-rich protein or PIGEA-14, is a highly conserved 126 amino acid protein that plays a crucial role in inhibiting β-catenin-mediated transcriptional activation by competing with LEF-1 for binding to β-catenin. This function is vital for regulating Wnt signaling pathways, which are essential for various cellular processes, including cell proliferation and differentiation. Chibby is primarily localized in the nucleus and the Golgi apparatus within the trans-Golgi network, and acts as a homodimer. Interaction with the 14-3-3 protein leads to sequestration of Chibby in the cytoplasm, forming a stable complex with β-catenin that facilitates nuclear export of β-catenin. While Chibby is widely expressed, particularly high levels are found in skeletal muscle, heart, placenta, and kidney. Down-regulation of Chibby has been observed in thyroid and metastatic uterine tumors, indicating that the gene encoding Chibby may function as a tumor suppressor, highlighting potential importance in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Chibby Antibody (C-10) References:
- Chibby, a nuclear beta-catenin-associated antagonist of the Wnt/Wingless pathway. | Takemaru, K., et al. 2003. Nature. 422: 905-9. PMID: 12712206
- PIGEA-14, a novel coiled-coil protein affecting the intracellular distribution of polycystin-2. | Hidaka, S., et al. 2004. J Biol Chem. 279: 35009-16. PMID: 15194699
- Is the gene encoding Chibby implicated as a tumour suppressor in colorectal cancer ? | Gad, S., et al. 2004. BMC Cancer. 4: 31. PMID: 15245581
- TC1 (C8orf4) enhances the Wnt/beta-catenin pathway by relieving antagonistic activity of Chibby. | Jung, Y., et al. 2006. Cancer Res. 66: 723-8. PMID: 16424001
- Reduced expression of beta-catenin inhibitor Chibby in colon carcinoma cell lines. | Schuierer, MM., et al. 2006. World J Gastroenterol. 12: 1529-35. PMID: 16570344
- Chibby promotes adipocyte differentiation through inhibition of beta-catenin signaling. | Li, FQ., et al. 2007. Mol Cell Biol. 27: 4347-54. PMID: 17403895
- The intrinsically disordered TC-1 interacts with Chibby via regions with high helical propensity. | Gall, C., et al. 2007. Protein Sci. 16: 2510-8. PMID: 17905836
- Chibby cooperates with 14-3-3 to regulate beta-catenin subcellular distribution and signaling activity. | Li, FQ., et al. 2008. J Cell Biol. 181: 1141-54. PMID: 18573912
- Fine-tuning of nuclear-catenin by Chibby and 14-3-3. | Takemaru, K., et al. 2009. Cell Cycle. 8: 210-3. PMID: 19158508
- Chibby 1: a new component of β-catenin-signaling in chronic myeloid leukemia. | Mancini, M., et al. 2017. Oncotarget. 8: 88244-88250. PMID: 29152155
- Chibby is a weak regulator of β-catenin activity in gastric epithelium. | Li, FQ., et al. 2019. J Cell Physiol. 234: 1871-1879. PMID: 30063079
- Combined Chibby and β-Catenin Predicts Clinical Outcomes in Patients with Hepatocellular Carcinoma. | Tsai, MC., et al. 2020. Int J Mol Sci. 21: PMID: 32192213
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Chibby Antibody (C-10) | sc-393295 | 200 µg/ml | $322.00 | |||
Chibby Antibody (C-10) AC | sc-393295 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Chibby Antibody (C-10) HRP | sc-393295 HRP | 200 µg/ml | $322.00 | |||
Chibby Antibody (C-10) FITC | sc-393295 FITC | 200 µg/ml | $336.00 | |||
Chibby Antibody (C-10) PE | sc-393295 PE | 200 µg/ml | $349.00 | |||
Chibby Antibody (C-10) Alexa Fluor® 488 | sc-393295 AF488 | 200 µg/ml | $364.00 | |||
Chibby Antibody (C-10) Alexa Fluor® 546 | sc-393295 AF546 | 200 µg/ml | $364.00 | |||
Chibby Antibody (C-10) Alexa Fluor® 594 | sc-393295 AF594 | 200 µg/ml | $364.00 | |||
Chibby Antibody (C-10) Alexa Fluor® 647 | sc-393295 AF647 | 200 µg/ml | $364.00 | |||
Chibby Antibody (C-10) Alexa Fluor® 680 | sc-393295 AF680 | 200 µg/ml | $364.00 | |||
Chibby Antibody (C-10) Alexa Fluor® 790 | sc-393295 AF790 | 200 µg/ml | $364.00 | |||
Chibby (C-10) Neutralizing Peptide | sc-393295 P | 100 µg/0.5 ml | $69.00 |