CaMKII delta (h): 293T Lysate: sc-115074

CaMKIIδ (h): 293T Lysate is derived from the 293T cell line, a human embryonic kidney cell line modified to express the SV40 large T antigen, which enhances its transfection efficiency and protein expression capabilities. This lysate is enriched with human CaMKIIδ, a subtype of the Ca2+/calmodulin-dependent protein kinase II (CaMKII) family, known for its crucial role in various cellular processes, including signal transduction, gene expression, and synaptic plasticity. CaMKIIδ is particularly important in the regulation of cardiac function and neuronal signaling, where it phosphorylates multiple target proteins in response to calcium influx. In research, CaMKIIδ (h): 293T Lysate is utilized to study the molecular mechanisms of CaMKIIδ activation and its downstream effects. Researchers employ this lysate in Western blotting to detect and quantify CaMKIIδ expression levels under different experimental conditions, providing insights into its regulation and activity. The lysate is also used in kinase activity assays to measure the phosphorylation of specific substrates by CaMKIIδ, helping to explain its role in signaling pathways. Additionally, co-immunoprecipitation assays using CaMKIIδ (h): 293T Lysate are conducted to identify protein-protein interactions involving CaMKIIδ, which aids in understanding its participation in complex cellular networks. The lysate is also valuable in studying the effects of CaMKIIδ on cellular processes such as cardiac muscle contraction and synaptic transmission, contributing to a deeper understanding of its physiological roles.