Date published: 2025-9-7

00800 4573 8000

SCBT Portrait Logo
Seach Input

c-Fos Anticorpo (E-8): sc-166940

4.0(47)
Escrever uma avaliaçãoFazer uma pergunta

Fichas de dados
  • c-Fos Anticorpo E-8é um anticorpo monoclonal produzido em camundongo IgG1 κ c-Fos anticorpo, citado em 171 publicações, fornecido em 200 µg/ml
  • específico para um epítopo mapeado entre os aminoácidos 128-152 dentro de uma região interna de c-Fos de human origem
  • c-Fos Anticorpo (E-8) é recomendado para a detecção de c-Fos, Fos B, Fra-1 and Fra-2 de mouse, rat, human, zebrafish e de origem WB, IP, IF, IHC(P) e ELISA; Reage também com outras espécies, incluindo and equine, canine, bovine, porcine and avian
  • Anticorpo anti-c-Fos O anticorpo anti-c-Fos (E-8) está disponível conjugado com agarose para IP; HRP para WB, IHC(P) e ELISA; e com fioeritrina ou FITC para IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 para WB (RGB), IF, IHC(P) e FCM, e para utilização com sistemas de imagiologia fluorescente RGB, tais como iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • disponível como reagente TransCruz para ensaios de Gel Supershift e uso em ChIP (sc-166940 X, 200 µg/0.1 ml)
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de c-Fos (E-8): sc-166940.
  • m-IgG Fc BP-HRP e m-IgG1 BP- HRP são os reagentes de deteção secundários preferidos para c-Fos Anticorpo (E-8) para aplicações WB and IHC(P). Estes reagentes são agora oferecidos em conjuntos com c-Fos Anticorpo (E-8) (ver informações para encomenda abaixo).

LINKS RÁPIDOS

VEJA TAMBÉM

O anticorpo c-Fos (E-8) é um anticorpo monoclonal de camundongo IgG1 κ que detecta a proteína c-Fos de origem de peixe-zebra (m, r, h, zebrafish) por WB, IP, IF, IHC(P) e ELISA. O anticorpo c-Fos (E-8) está disponível tanto na forma de anticorpo anti-c-Fos não conjugado quanto em múltiplas formas conjugadas de anticorpo anti-c-Fos, incluindo agarose, HRP, PE, FITC e múltiplos conjugados de Alexa Fluor®. O oncogene c-Fos foi inicialmente detectado em dois isolados independentes do vírus da osteossarcoma em camundongos e um vírus de nefroblastoma aviário. O homólogo celular, c-Fos, codifica uma fosfoproteína nuclear que é rapidamente e transitoriamente induzida por uma variedade de agentes e funciona como um regulador transcricional para vários genes. Em contraste com as proteínas c-Jun, que formam homodímeros e heterodímeros que se ligam a elementos de resposta de DNA específicos, as proteínas c-Fos são apenas ativas como heterodímeros com membros da família de genes Jun. Homólogos funcionais de c-Fos incluem os genes Fra-1, Fra-2 e Fos B. Além disso, membros selecionados da família ATF/CREB podem formar dímeros de zíper de leucina com Fos e Jun. Diferentes dímeros exibem especificidade e afinidade diferenciais para sítios AP-1 e CRE.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

c-Fos Anticorpo (E-8)

sc-166940
200 µg/ml
$316.00

Pacote do c-Fos (E-8): m-IgG Fc BP-HRP

sc-527195
200 µg Ab; 10 µg BP
$354.00

Pacote do c-Fos (E-8): m-IgG1 BP-HRP

sc-532568
200 µg Ab; 20 µg BP
$354.00

c-Fos Anticorpo (E-8) X

sc-166940 X
200 µg/0.1 ml
$316.00

c-Fos Anticorpo (E-8) AC

sc-166940 AC
500 µg/ml, 25% agarose
$416.00

c-Fos Anticorpo (E-8) HRP

sc-166940 HRP
200 µg/ml
$316.00

c-Fos Anticorpo (E-8) FITC

sc-166940 FITC
200 µg/ml
$330.00

c-Fos Anticorpo (E-8) PE

sc-166940 PE
200 µg/ml
$343.00

c-Fos Anticorpo (E-8) Alexa Fluor® 488

sc-166940 AF488
200 µg/ml
$357.00

c-Fos Anticorpo (E-8) Alexa Fluor® 546

sc-166940 AF546
200 µg/ml
$357.00

c-Fos Anticorpo (E-8) Alexa Fluor® 594

sc-166940 AF594
200 µg/ml
$357.00

c-Fos Anticorpo (E-8) Alexa Fluor® 647

sc-166940 AF647
200 µg/ml
$357.00

c-Fos Anticorpo (E-8) Alexa Fluor® 680

sc-166940 AF680
200 µg/ml
$357.00

c-Fos Anticorpo (E-8) Alexa Fluor® 790

sc-166940 AF790
200 µg/ml
$357.00

c-Fos (E-8) peptídeo neutralizante

sc-166940 P
100 µg/0.5 ml
$68.00

Has E-8 already been tested for the iDisco protocol? If so, at what dilution? Many thanks!

Asked by: Vimast
Thank you for your question. sc-166940 c-Fos (E-8) has not been used in a iDisco protocol.
Answered by: BlakeJ
Date published: 2025-04-01

What dilution do you recommend starting with for flow cytometry using the fluorophore-conjugated version of this antibody? Are there any publications using this antibody for flow cytometry?

Asked by: dr9004
Thank you for your question. We do not have any publications but we do warranty the antibody for flow cytometry. We recommend using 1 µg normal immunoglobulins per 1 million cells. Here is our protocol: https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2023-09-19

sc-166940这只c-fos的抗体,我染小鼠脑组织的冰冻切片,0.3%Triton打孔10分钟,山羊血清室温封闭一小时,一抗4℃过夜,使用Alexa Fluo 488的二抗室温避光孵育1小时,但无阳性信号出现,请问问题出在哪里了呢?

Asked by: pangpangmao
感谢您的提问。我们很遗憾地获悉,这种抗体没有达到您的预期。我们希望你的研究取得成功。请联系我们的技术支持部门,亚洲技术支持电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”。 Thank you for your question. We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Please contact our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody.
Answered by: SCBT Representative
Date published: 2022-07-07

What temperature is suitable for storing and reusing c-fos antibody (Anti-Fos/c-Fos Antibody (E-8) X) following dilution, 4C, or -20C freezer?

Asked by: Parisa
We do not recommend freezing our antibodies. Recommended storage is 4C.
Answered by: Technical Service
Date published: 2022-04-07

I have a question about Anti-Fos/c-Fos Antibody (E-8) X (catalog: sc-166940 X) for western blot analysis. I would like to know if it is possible to use both HRP and fluorescent dye conjugated secondary antibodies for detecting the sc-166940 X antibody.

Asked by: Parisa
Thank you for your question. This primary antibody can be detected by either fluorescence-, or by an HRP-based secondary detection. Our secondary binding proteins m-IgG1 BP-HRP: sc-525408 and m-IgG1 BP-CFL 488: sc-533661 have been optimised for detecting primary mouse IgG1 antibodies including this one.
Answered by: Tech Support Europe
Date published: 2022-02-17

Hello, The c-Fos Antibody (K-25): sc-253 was discontinued and replaced by c-Fos Antibody (E-8): sc-166940? In what way are they the same? sc-253 has been shown to work for fish c-fos detection, so will sc-166940 work in the same way? Cheers!

Asked by: Yatz2020
Thank you for your question. c-Fos Antibody (E-8): sc-166940 is a mouse monoclonal IgG1 antibody that was raised against the same peptide antigen that was used to develop the rabbit polyclonal antibody, c-Fos Antibody (K-25): sc-253. The mouse monoclonal antibody has also been published in zebrafish samples, and is recommended to work for zebrafish. For some examples of literature using this mouse monoclonal antibody in zebrafish, please refer on our website to the product citations with PMID's: 30222997, 29626809 and 29061647.
Answered by: Technical Support
Date published: 2020-03-12

Is the c-Fos Antibody (E-8): sc-166940 tested for flow cytometry or if you could suggest any published references that have used this antibody for flow cytometry? Thanks!!

Asked by: Hema
The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-08-20

I would like to determine the c-Fos expression in the mouse whole brain cleared with CUBIC or iDISCO. Have you tried to use E-8 for that?

Asked by: Atsu
Thank you for your question. We have not tested this antibody in whole brain tissues, or using either CUBIC or iDISCO.
Answered by: Technical Support
Date published: 2019-07-30
  • y_2025, m_8, d_29, h_8CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_20
  • loc_pt_PT, sid_166940, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 104ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from Doesn't workWe could not detect any signal on PFA fixated mouse brain slice after IHC
Date published: 2024-04-05
Rated 4 out of 5 by from c-fosGood antibody for detecting c-fos in mouse mice tissue. Dilution: 1:100, perfect staining.
Date published: 2024-01-16
Rated 1 out of 5 by from Did not work in mouse brainNo staining was detected in frozen mouse brain slices fixed in PFA
Date published: 2023-01-05
Rated 1 out of 5 by from Does not work for ChIP in mouse neuronsI tested this antibody for ChIP in mouse primary neurons after stimulation, and there was no detectable enrichment by qPCR. I had used the same protocol for Egr1 ChIP (a similar inducible transcription factor) and it works nicely. Also, quite some background in WB.
Date published: 2022-03-09
Rated 5 out of 5 by from It can be usedIt can be used for osteoclasts which obtained from mouse.
Date published: 2021-07-21
Rated 5 out of 5 by from 条带清晰,效价高之前用的A牌的抗体,就是不出,然后就申请了santa cruz的,想试试,没想到出图效果特别好,条带清晰,效价高
Date published: 2021-07-09
Rated 1 out of 5 by from Per immunofluorescenza non funziona!Ho provato questo anticorpo in immunofluorescenza su spinal cord di topo criostatato (diluizione 1:100), purtroppo senza alcun risultato positivo. Proverò ad utilizzarlo anche per wester blot
Date published: 2020-11-03
Rated 5 out of 5 by from Amazing in CLARITYI use this antibody in CLARITY and it has consistently produced the best images I have ever taken.
Date published: 2020-03-10
  • y_2025, m_8, d_29, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_47
  • loc_pt_PT, sid_166940, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 19ms
  • REVIEWS, PRODUCT
c-Fos Anticorpo (E-8) is rated 4.0 out of 5 by 47.
  • y_2025, m_8, d_29, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_47
  • loc_pt_PT, sid_166940, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 103ms
  • REVIEWS, PRODUCT