Date published: 2025-9-7

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c-Fos Anticorpo (D-1): sc-8047

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Fichas de dados
  • c-Fos Anticorpo D-1é um anticorpo monoclonal produzido em camundongo IgG2b κ, citado em 103 publicações, fornecido em 200 µg/ml
  • raised against amino acids 210-335 mapping at the C-terminus of c-Fos of human origin
  • Anti-c-Fos Anticorpo (D-1) é recomendado para a detecção de c-Fos de mouse, rat e human origem em WB, IP, IF, IHC(P) e ELISA
  • c-Fos O anticorpo (D-1) está disponível conjugado com Alexa Fluor® 546 ou Alexa Fluor® 594 para WB (RGB), IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • disponível como reagente TransCruz para ensaios de Gel Supershift e uso em ChIP (sc-8047 X, 200 µg/0.1 ml)
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de c-Fos (D-1): sc-8047.
  • m-IgG Fc BP-HRP e m-IgGκ BP-HRP são os reagentes de deteção secundários preferidos para c-Fos Anticorpo (D-1) para aplicações WB and IHC(P). Estes reagentes são agora oferecidos em conjuntos com c-Fos Anticorpo (D-1) (ver informações para encomenda abaixo).

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VEJA TAMBÉM

O anticorpo c-Fos (D-1) é um anticorpo monoclonal de camundongo IgG2b κ que detecta c-Fos de origem de camundongo, rato e humano por WB, IP, IF, IHC(P) e ELISA. O anticorpo c-Fos (D-1) está disponível tanto na forma de anticorpo anti-c-Fos conjugado quanto na forma não conjugada. O oncogene c-Fos foi inicialmente detectado em dois isolados independentes do vírus da osteossarcoma murino e um vírus de nefroblastoma aviário. O homólogo celular, c-Fos, codifica uma fosfoproteína nuclear que é rapidamente e transitoriamente induzida por uma variedade de agentes e funciona como um regulador transcricional para vários genes. Em contraste com as proteínas c-Jun, que formam homodímeros e heterodímeros que se ligam a elementos de resposta de DNA específicos, as proteínas c-Fos são apenas ativas como heterodímeros com membros da família de genes Jun. Homólogos funcionais de c-Fos incluem os genes Fra-1, Fra-2 e Fos B. Além disso, membros selecionados da família ATF/CREB podem formar dímeros de zíper de leucina com Fos e Jun. Diferentes dímeros exibem especificidade e afinidade diferenciais para sítios AP-1 e CRE.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Referencias do c-Fos Anticorpo (D-1):

  1. A proteína quinase D2 potencia a sinalização MEK/ERK/RSK, a acumulação de c-Fos e a síntese de ADN induzida pela bombesina em células Swiss 3T3.  |  Sinnett-Smith, J., et al. 2007. J Cell Physiol. 211: 781-90. PMID: 17226786
  2. Análise imunohistoquímica da expressão dos genes p53, cyclinD1, RB1, c-fos e N-ras no carcinoma hepatocelular no Irão.  |  Moghaddam, SJ., et al. 2007. World J Gastroenterol. 13: 588-93. PMID: 17278226
  3. Isolamento e caraterização de fra-2, um membro adicional da família do gene fos.  |  Nishina, H., et al. 1990. Proc Natl Acad Sci U S A. 87: 3619-23. PMID: 2110368
  4. Um papel direto de c-fos na transcrição de genes dependentes de AP-1.  |  Herrera, R., et al. 1990. Cell Growth Differ. 1: 483-90. PMID: 2126190
  5. O produto de um gene relacionado com fos, fra-1, liga-se cooperativamente ao sítio AP-1 com Jun: o fator de transcrição AP-1 é composto por múltiplos complexos proteicos.  |  Cohen, DR., et al. 1989. Genes Dev. 3: 173-84. PMID: 2497053
  6. Um retrovírus transformador aviário isolado de um nefroblastoma que transporta o gene fos como oncogene.  |  Nishizawa, M., et al. 1987. J Virol. 61: 3733-40. PMID: 2824811
  7. O proto-oncogene humano c-jun codifica uma proteína de ligação ao ADN com propriedades estruturais e funcionais do fator de transcrição AP-1.  |  Bohmann, D., et al. 1987. Science. 238: 1386-92. PMID: 2825349
  8. Associação da cromatina e propriedades de ligação ao ADN do produto do proto-oncogene c-fos.  |  Renz, M., et al. 1987. Nucleic Acids Res. 15: 277-92. PMID: 3103098
  9. O complexo da proteína Fos está associado ao ADN em núcleos isolados e liga-se à celulose do ADN.  |  Sambucetti, LC. and Curran, T. 1986. Science. 234: 1417-9. PMID: 3491427
  10. Indução viral de osteossarcomas em ratinhos.  |  Finkel, MP., et al. 1966. Science. 151: 698-701. PMID: 5218049

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

c-Fos Anticorpo (D-1)

sc-8047
200 µg/ml
$316.00

Pacote do c-Fos (D-1): m-IgG Fc BP-HRP

sc-528213
200 µg Ab; 10 µg BP
$354.00

Pacote do c-Fos (D-1): m-IgGκ BP-HRP

sc-520536
200 µg Ab, 40 µg BP
$354.00

c-Fos Anticorpo (D-1) X

sc-8047 X
200 µg/0.1 ml
$316.00

c-Fos Anticorpo (D-1) Alexa Fluor® 546

sc-8047 AF546
200 µg/ml
$357.00

c-Fos Anticorpo (D-1) Alexa Fluor® 594

sc-8047 AF594
200 µg/ml
$357.00

c-Fos Anticorpo (D-1) Alexa Fluor® 680

sc-8047 AF680
200 µg/ml
$357.00

c-Fos Anticorpo (D-1) Alexa Fluor® 790

sc-8047 AF790
200 µg/ml
$357.00

Whether the antibody is suitable for the detection of liver tissue western blot of male rats, or recommend the appropriate antibody

Asked by: ZiLi
Thank you for your question.sc-8047 is recommended for WB, IP, IF, IHC (P) and ELISA to detect c-Fos from mouse, rat and human sources.It can be used in rat samples for WB experiment.If you have any further questions, please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Jiajun Z
Date published: 2022-10-31

what is the best replacement for Sc-52

Asked by: ismaila
Thank you for your question. This is one of the most popular replacement antibodies for that now discontinued polyclonal antibody, sc-52. However, we offer several different options. Please contact our Technical Service Department for a recommendation based on your intended research application(s) and species reactivity.
Answered by: Technical Support
Date published: 2019-05-25

I used immunofluorescence staining in the hypothalamus of rat, and as a result c-fos was expressed in the cytoplasm. It's not expressed in the nucleus. Is it correct? 0 answers

Asked by: Emma Li
Thank you for your question. According to the Human Protein Atlas, c-Fos (FOS) is localized to the nucleoplasm. Nuclear and cytoplasmic expression may be detected in most tissues. https://www.proteinatlas.org/ENSG00000170345-FOS/pathology
Answered by: Technical Support
Date published: 2019-05-25

Can this antibody be used for the detection of c-fos in the macaque monkey. If not, which of your antibodies would you recommend?

Asked by: myprefrontal
Thank you for your question. We have not tested this antibody for reactivity with macaque, and are not aware of any publications citing use of the antibody in that species. We do offer an alternative antibody, c-Fos (6-2H-2F): sc-447, that has been published at least twice for detection of c-Fos in monkey. Please see PMID's: 9108029 and 11937718 for those references. A link to that product page on our website is below. https://www.scbt.com/scbt/product/c-fos-antibody-6-2h-2f
Answered by: Technical Support
Date published: 2017-11-01

Can I still buy monoclonal antibodies from this company?

Asked by: KarenLindquist
Thank you for your question! Yes, you can certainly buy monoclonal antibodies from us! Please contact us at any time and we'd be glad to help you! You can reach us by phone at 800-457-3801 or by email at scbt@scbt.com or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-05-29

Could you please advise if this antibody is able to detect Fra proteins as is the case with the discontinued sc 253.

Asked by: Conor
Thank you for your question. The equivalent replacement mouse monoclonal antibodies to sc-253 are sc-166940: c-Fos (E-8) and sc-271243: c-Fos (C-10). Both of these mouse monoclonal antibodies are raised against the same peptide antigen used to generate the polyclonal antibody, sc-253. These recommended replacement mouse monoclonal antibodies are also recommended for detection of c-Fos, Fos B, Fra-1 and Fra-2 of mouse, rat, human, zebrafish and avian origin. Links to these products are provided below for your reference. https://www.scbt.com/scbt/product/c-fos-antibody-e-8 https://www.scbt.com/scbt/product/c-fos-antibody-c-10
Answered by: Technical Support
Date published: 2017-01-19

I want to detect c-fos in my porcine samples. Will this antibody work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2016-12-23
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Rated 3 out of 5 by from Nucleus hypocampus mark but not hypothalamusWe tested this antibody 1:1000 in cryosectioned rat brains. Strong nuclear staining is observed in the hypocampus but not in the suprachiasmatic nucleus (SCN) where there should be staining since these animals were euthanized at the begining of their resting phase. Using cFos antibodies from other companies we were able to acquire a positive staining for the SCN. This antibody will not be usefull for us since it is not comparable with IHC using other antibodies.
Date published: 2025-04-04
Rated 5 out of 5 by from ExcellentI bought this antibody a few years ago and the result is good and clear.
Date published: 2021-11-08
Rated 5 out of 5 by from -We examined the effects of a 3-week treatment of olanzapine (Olz) on c-Fos protein expression in stress-relevant brain sub/ regions, its relationship with isolation-induced behavioral changes, and potential sites of Olz action on control and male rats exposed to 6 weeks of chronic social isolation an animal model of depression.
Date published: 2018-12-07
Rated 5 out of 5 by from 功能很齐全也很棒我两个月前购买了这个产品,用它来做免疫荧光看的非常清楚,我想再买一盒接着做,同时我最满意的是它的性价比,很实惠也很实用,值得购买
Date published: 2018-05-21
Rated 2 out of 5 by from It does not work wellIt did not work as well as other company's ab when I tested it by 1: 500.
Date published: 2018-04-03
Rated 2 out of 5 by from No nuclear stainingLevel of expression appears comparable to other fos antibodies, but does not stain the the nucleus and non-specific binding is present at high concentrations.
Date published: 2018-03-14
Rated 1 out of 5 by from Not satisfied!I tried a free sample of this antibody on free floating mouse brain sections and it didn't work. I used a concentration of 1:50 for the primary antibody, and 1:300 for secondary antibody (anti-mouse 488) and I didn't got any signal. Really not satisfied.
Date published: 2018-02-23
Rated 5 out of 5 by from 功能很出色我上一个月染得的fos,很出色,背景很清晰,很清晰。我上一个月染得的fos,很出色,背景很清晰,很清晰。
Date published: 2018-02-05
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c-Fos Anticorpo (D-1) is rated 3.9 out of 5 by 20.
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