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c-Fos Antibody (C-10) - Western Blotting - Image 379667
Anticorpo c-Fos (C-10): sc-271243. Análise por Western blot da expressão de c-Fos em lisados de células inteiras de A-431 (A), Y79 (B), SK-BR-3 (C), C2C12 (D) e TK-1 (E).
Anticorpo c-Fos (C-10): sc-271243. Análise por Western blot da expressão de c-Fos em lisados de células inteiras de A-431 (A), Y79 (B), SK-BR-3 (C), C2C12 (D) e TK-1 (E).

c-Fos Anticorpo (C-10): sc-271243

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Fichas de dados
  • c-Fos Anticorpo C-10é um anticorpo monoclonal produzido em camundongo IgG2a κ, citado em 71 publicações, fornecido em 200 µg/ml
  • específico para um epítopo mapeado entre os aminoácidos 120-155 dentro de uma região interna de c-Fos de human origem
  • c-Fos Anticorpo (C-10) é recomendado para a detecção de c-Fos, Fos B, Fra-1 and Fra-2 de mouse, rat, human e de origem WB, IP, IF, IHC(P) e ELISA; Reage também com outras espécies, incluindo and equine, canine, bovine, porcine and avian
  • dispnonível como reagente TransCruz para o uso em ChIP (sc-271243 X, 200 µg/0.1 ml)
  • Anticorpo anti-c-Fos O anticorpo anti-c-Fos (C-10) está disponível conjugado com agarose para IP; HRP para WB, IHC(P) e ELISA; e com fioeritrina ou FITC para IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 para WB (RGB), IF, IHC(P) e FCM, e para utilização com sistemas de imagiologia fluorescente RGB, tais como iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de c-Fos (C-10): sc-271243.
  • m-IgG Fc BP-HRP e 2a BP-HRP">m-IgG2a BP-HRP são os reagentes de deteção secundários preferidos para c-Fos Antibody (C-10) para aplicações WB e IHC(P). Estes reagentes são agora oferecidos em conjuntos com c-Fos Antibody (C-10)(ver informações de encomenda abaixo).

LINKS RÁPIDOS

Produtos auxiliares
Descricao
Identificacao do gene
Ficha de dados e Protocolos
Informações de investigação

VEJA TAMBÉM

Outros c-Fos Anticorpos incluindo Fos B

O anticorpo c-Fos (C-10) é um anticorpo monoclonal de camundongo IgG2a κ que detecta c-Fos, Fos B, Fra-1 e Fra-2 de origem de camundongo, rato e humano por WB, IP, IF, IHC(P) e ELISA. O anticorpo c-Fos (C-10) está disponível na forma de anticorpo anti-c-Fos não conjugado, bem como em múltiplas formas conjugadas de anticorpo anti-c-Fos, incluindo agarose, HRP, PE, FITC e múltiplos conjugados Alexa Fluor®. O oncogene c-Fos foi inicialmente detectado em dois isolados independentes do vírus da osteossarcoma em camundongo e um vírus de nefroblastoma aviário. O homólogo celular, c-Fos, codifica uma fosfoproteína nuclear que é induzida rapidamente e de forma transitória por uma variedade de agentes e funciona como um regulador transcricional para vários genes. Em contraste com as proteínas c-Jun, que formam homodímeros e heterodímeros que se ligam a elementos de resposta de DNA específicos, as proteínas c-Fos são apenas ativas como heterodímeros com membros da família de genes Jun. Homólogos funcionais de c-Fos incluem os genes Fra-1, Fra-2 e Fos B. Além disso, membros selecionados da família ATF/CREB podem formar dímeros de zíper de leucina com Fos e Jun. Diferentes dímeros exibem especificidade e afinidade diferenciais para sítios AP-1 e CRE.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

c-Fos Anticorpo (C-10)

sc-271243
200 µg/ml
$316.00

Pacote do c-Fos (C-10): m-IgG Fc BP-HRP

sc-525793
200 µg Ab; 10 µg BP
$354.00

Pacote do c-Fos (C-10): m-IgG2a BP-HRP

sc-546417
200 µg Ab; 10 µg BP
$354.00

c-Fos Anticorpo (C-10) X

sc-271243 X
200 µg/0.1 ml
$316.00

c-Fos Anticorpo (C-10) AC

sc-271243 AC
500 µg/ml, 25% agarose
$416.00

c-Fos Anticorpo (C-10) HRP

sc-271243 HRP
200 µg/ml
$316.00

c-Fos Anticorpo (C-10) FITC

sc-271243 FITC
200 µg/ml
$330.00

c-Fos Anticorpo (C-10) PE

sc-271243 PE
200 µg/ml
$343.00

c-Fos Anticorpo (C-10) Alexa Fluor® 488

sc-271243 AF488
200 µg/ml
$357.00

c-Fos Anticorpo (C-10) Alexa Fluor® 546

sc-271243 AF546
200 µg/ml
$357.00

c-Fos Anticorpo (C-10) Alexa Fluor® 594

sc-271243 AF594
200 µg/ml
$357.00

c-Fos Anticorpo (C-10) Alexa Fluor® 647

sc-271243 AF647
200 µg/ml
$357.00

c-Fos Anticorpo (C-10) Alexa Fluor® 680

sc-271243 AF680
200 µg/ml
$357.00

c-Fos Anticorpo (C-10) Alexa Fluor® 790

sc-271243 AF790
200 µg/ml
$357.00

c-Fos (C-10) peptídeo neutralizante

sc-271243 P
100 µg/0.5 ml
$68.00

Because there is no way to know if it works on mouse tissue until we try. To avoid anti-mouse secondary, it would be nice to try anti-Fos conjugated to Alexa 488 or 594. Do you have small samples of these?

Asked by: Svet
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

How can I get a free sample? I want to test it before making purchases. Thanks!

Asked by: kisong
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-04-29

Is there a mouse-on-mouse issue, when used on mouse tissue?

Asked by: Berry2019
Thank you for your question. There are numerous publications citing use of this mouse monoclonal c-Fos antibody on mouse tissue. Typically, unless your tissues are rich with endogenous IgG there should not be a lot of non-specific binding using a mouse monoclonal antibody on mouse tissue. However, we do offer this antibody directly conjugated, so you can avoid having to use any anti-mouse IgG secondary antibody for detection, and have the most specific result.
Answered by: Technical Support
Date published: 2019-03-15

Hello, I would like to know the price of this product (Is c-Fos (C-10): sc-271243 monoclonal antibody). Thank you!

Asked by: Aline F Melo
Thank you for your question. Please visit our website for up-to-date pricing information: https://www.scbt.com/scbt/product/c-fos-antibody-c-10 ?requestFrom=search
Answered by: Technical Support
Date published: 2019-01-06

What is the expiration date of this ab kept at 4C?

Asked by: nf85
All of our products are warrantied for one year from the date purchased. We do not provide expiration dates for our antibodies. If properly stored at 4C it can continue to be viable for several years if not longer.
Answered by: Technical Support
Date published: 2018-12-21

这个抗体已经买了一年半了，现在还能用吗？

Asked by: wwwwwwwww
感谢您的提问。抱歉这个没法确认，您可以试验试试。更多问题，欢迎通过：电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your inquiry. Sorry that we can not sure, maybe you can do a test. For more questions, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2017-11-01

Is c-Fos (C-10): sc-271243 monoclonal antibody appropriate for use in IHC with paraffin-embedded sections?

Asked by: jenniferc
Thank you for your question. Yes, c-Fos (C-10): sc-271243 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 10
Date published: 2017-01-10
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Rated 5 out of 5 by from Works correctly.We have tested the antibody for IF at a 1/500 concentration and Alexa secondary antibodies at 1/500. In our hands, it works correctly. Images of mice injected with a hypertonic saline solution intraperitoneally.
Date published: 2025-03-05
Rated 4 out of 5 by from mouse monoclonal anti-c-Fosmouse monoclonal anti-c-Fos (sc-271243) Santa Cruz Biotechnology; diluted 1:1000
Date published: 2021-05-11
Rated 4 out of 5 by from Worked for mouse brain tissueI tried c-Fos ab C-10, D-1, E-8 at the same time in PFA-fixed flozen mouse brain and found only C-10 worked well. I applied 1:500 ~ 1:8000 dilution in 4°C, over night for floated 40um thick-sections. 1:8000 dilution was better for me.
Date published: 2021-02-18
Rated 1 out of 5 by from It is not useful for immunohistochemitryWe processed sections of the same subject with different dilutions and with a polyclonal antibody that has resulted very good for our purposes. The monoclonal c-Fos (C-10) did not give a good immunostaining. Moreover, it uncovered structures that look like blood vessels and obstruct a good appreciation of the c-Fos positive cells. It is important to indicate that such vessels were not vissible with our reference antibody.
Date published: 2018-04-27
Rated 5 out of 5 by from Worked great for IFI was looking for a robust c-Fos antibody for immuno-staining in mouse brain tissue. This one worked like a champ. I used 1:300 in PBST, RT o.n. for 100um slice. For secondary, I used Invitrogen isotype specific Alexa 647 A-21241 1:300 in PBST, RT o.n. and other secondary abs yielded nonspecific signals.
Date published: 2018-03-21
Rated 5 out of 5 by from Best SCBT alternative to sc-52We tried every alternative and this is by far the best. Great staining at 1:500.
Date published: 2018-03-14
Rated 5 out of 5 by from excellent almost same as sc-52it worked great on brain sections at 1:500 dilution, I feel better than the previous rabbit poly clonal
Date published: 2017-05-18
Rated 5 out of 5 by from goodsIts specificity for epitope mapping between amino acids 120-155 in the internal region of c-Fos of human origin is very clear and very useful
Date published: 2017-01-17
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c-Fos Anticorpo (C-10) is rated 4.3 out of 5 by 14.
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