ATF-1/ATF1 Antibody (2A9/8): sc-53172

ATF-1 Antibody (2A9/8) is a mouse monoclonal IgG2a antibody that detects ATF-1 in mouse, rat, human, and S. pombe by western blotting (WB) and immunoprecipitation (IP). ATF-1 is a crucial member of the ATF/CREB transcription factor family, which plays a significant role in regulating gene expression in response to various cellular signals. ATF-1 is primarily located in the nucleus, where ATF-1 binds to specific DNA sequences known as cAMP response elements (CRE) to modulate transcription. Nuclear localization of ATF-1 is vital for function, as ATF-1 interacts with other transcriptional regulators and chromatin remodeling complexes, thereby influencing the transcription of target genes involved in processes such as cell growth, differentiation, and stress responses. Notably, ATF-1′s ability to form homodimers and heterodimers with other family members, such as ATF-2 and c-Jun, enhances transcriptional activity and expands regulatory potential. Furthermore, ATF-1 is phosphorylated by protein kinase A (PKA), which is essential for activation and subsequent gene regulation. ATF-1 (2A9/8) monoclonal antibody is an invaluable tool for researchers studying the intricate mechanisms of gene regulation and the role of ATF-1 in various biological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ATF-1/ATF1 Antibody (2A9/8) References:

1. Inhibition of tumor growth and metastasis of human melanoma by intracellular anti-ATF-1 single chain Fv fragment.  |  Jean, D., et al. 2000. Oncogene. 19: 2721-30. PMID: 10851072
2. Genetic characterization of angiomatoid fibrous histiocytoma identifies fusion of the FUS and ATF-1 genes induced by a chromosomal translocation involving bands 12q13 and 16p11.  |  Waters, BL., et al. 2000. Cancer Genet Cytogenet. 121: 109-16. PMID: 11063792
3. Activation of extracellular signal-regulated kinases and CREB/ATF-1 mediate the expression of CCAAT/enhancer binding proteins beta and -delta in preadipocytes.  |  Belmonte, N., et al. 2001. Mol Endocrinol. 15: 2037-49. PMID: 11682632
4. Transduction of dominant negative ATF-1 suppresses the pX gene expression in joint fibroblastic cells derived from HTLV-I transgenic rats.  |  Ishizu, A., et al. 2003. Exp Mol Pathol. 74: 309-13. PMID: 12782019
5. Transactivation of the EGF receptor and a PI3 kinase-ATF-1 pathway is involved in the upregulation of NOX1, a catalytic subunit of NADPH oxidase.  |  Fan, C., et al. 2005. FEBS Lett. 579: 1301-5. PMID: 15710429
6. Transcription factors RFX1/EF-C and ATF-1 associate with the adenovirus E1A-responsive element of the human proliferating cell nuclear antigen promoter.  |  Labrie, C., et al. 1995. Nucleic Acids Res. 23: 3732-41. PMID: 7479004
7. Precise localization on chromosome 12 of the ATF-1 gene by fluorescence in situ hybridization.  |  Desmaze, C., et al. 1994. Hum Genet. 93: 207-8. PMID: 8112749
8. Establishment of a new continuous clear cell sarcoma cell line. Morphological and cytogenetic characterization and detection of chimaeric EWS/ATF-1 transcripts.  |  Hiraga, H., et al. 1997. Virchows Arch. 431: 45-51. PMID: 9247632
9. Coordinate transactivation of the interleukin-2 CD28 response element by c-Rel and ATF-1/CREB2.  |  Butscher, WG., et al. 1998. J Biol Chem. 273: 552-60. PMID: 9417115
10. Comparison of HTLV-I basal transcription and expression of CREB/ATF-1/CREM family members in peripheral blood mononuclear cells and Jurkat T cells.  |  Newbound, GC., et al. 1999. J Acquir Immune Defic Syndr Hum Retrovirol. 20: 1-10. PMID: 9928723

The preferred CREB1 antibody is CREB1 Antibody (D-12): sc-377154.