ACADS Double Nickase Plasmid (h): sc-405458-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACADS Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ACADS Double Nickase Plasmid (h) and ACADS Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ACADS. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACADS Antibody (G-10): sc-365953
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACADS Double Nickase Plasmid (h) | sc-405458-NIC | 20 µg | $410.00 | |||
ACADS Double Nickase Plasmid (h2) | sc-405458-NIC-2 | 20 µg | $410.00 |
ACADS encodes short-chain acyl-CoA dehydrogenase, a mitochondrial flavoprotein that catalyzes the initial dehydrogenation step in β-oxidation of short-chain fatty acyl-CoAs, linking fatty acid catabolism to energy production during fasting and metabolic stress. By transferring electrons to the electron transfer flavoprotein system and downstream respiratory chain components, ACADS supports mitochondrial redox balance and efficient flux through fatty acid oxidation pathways. Altered ACADS function is associated with impaired short-chain fatty acid oxidation, metabolic acidosis, and neuromuscular symptoms observed in short-chain acyl-CoA dehydrogenase deficiency, making it relevant to studies of inborn errors of metabolism. ACADS also provides a useful node for investigating mitochondrial metabolism, oxidative stress responses, and lipid-derived signaling in human cells.
ACADS Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ACADS locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ACADS. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ACADS function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ACADS-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.