Zuotin-1 Antibody (A-12): sc-393426

Zuotin-1 Antibody (A-12) is a mouse monoclonal IgM antibody that detects Zuotin-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Zuotin-1 antibody (A-12) is available as the non-conjugated form. Zuotin-1, also known as DnaJ homolog subfamily C member 2 or M-phase phosphoprotein 11, plays a crucial role in cellular protein homeostasis as a ribosome-associated DnaJ molecular chaperone. Zuotin-1 contains one J domain and two SANT domains, which are essential for assisting proper folding of nascent polypeptides and preventing protein aggregation. Zuotin-1′s importance lies in its interaction with the HSP 70 chaperone machinery, where Zuotin-1 stimulates ATP hydrolysis, facilitating the protein folding process. This interaction is vital for maintaining cellular function, especially under stress conditions, as Zuotin-1 helps ensure proteins achieve correct conformation and function. Additionally, the presence of cysteine-rich regions and zinc finger motifs within the DnaJ family members enhances their ability to bind and stabilize unfolded proteins, making Zuotin-1 a key player in the cellular response to misfolded proteins.

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Zuotin-1 Antibody (A-12) References:

1. Ribosome-tethered molecular chaperones: the first line of defense against protein misfolding?  |  Craig, EA., et al. 2003. Curr Opin Microbiol. 6: 157-62. PMID: 12732306
2. Human Mpp11 J protein: ribosome-tethered molecular chaperones are ubiquitous.  |  Hundley, HA., et al. 2005. Science. 308: 1032-4. PMID: 15802566
3. The chaperones MPP11 and Hsp70L1 form the mammalian ribosome-associated complex.  |  Otto, H., et al. 2005. Proc Natl Acad Sci U S A. 102: 10064-9. PMID: 16002468
4. The diversity of the DnaJ/Hsp40 family, the crucial partners for Hsp70 chaperones.  |  Qiu, XB., et al. 2006. Cell Mol Life Sci. 63: 2560-70. PMID: 16952052
5. Zuotin, a DnaJ molecular chaperone, stimulates cap-independent translation in yeast.  |  Raychaudhuri, S., et al. 2006. Biochem Biophys Res Commun. 350: 788-95. PMID: 17027912
6. Cloning and chromosomal localization of a mouse cDNA with homology to the Saccharomyces cerevisiae gene zuotin.  |  Hughes, R., et al. 1995. Genomics. 29: 546-50. PMID: 8666407
7. Zuotin, a ribosome-associated DnaJ molecular chaperone.  |  Yan, W., et al. 1998. EMBO J. 17: 4809-17. PMID: 9707440

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Zuotin-1 Antibody (A-12) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Zuotin-1 Antibody (A-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Zuotin-1 Antibody (A-12) Specifications

Alternate Product Name: Zuotin-1

Product Clone Name: A-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 10-29

Epitope Target: Zuotin-1

Epitope Species: human

Product Reactivity/Detection: Zuotin-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Zuotin-1 Antibody (A-12): P: Blocking Peptide