Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ZSWIM8 CRISPR/Cas9 KO Plasmid (h): sc-411661
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZSWIM8 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZSWIM8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ZSWIM8 Antibody (G-5): sc-514204
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZSWIM8 CRISPR/Cas9 KO Plasmid (h) | sc-411661 | 20 µg | $397.00 |
Overview
ZSWIM8 (zinc finger SWIM-type containing 8) encodes an E3 ubiquitin ligase component that functions as a substrate adaptor within a Cullin-RING ubiquitination complex, helping control protein turnover in the nucleus and cytoplasm. A key role of ZSWIM8 is in small-RNA quality control, where it promotes target-directed microRNA degradation (TDMD) to tune miRNA abundance and downstream post-transcriptional gene regulation. Through these mechanisms, ZSWIM8 influences pathways linked to cell-state transitions, developmental programs, and stress-responsive transcriptional networks. Dysregulated ubiquitin-mediated proteostasis and miRNA homeostasis associated with altered ZSWIM8 activity have been investigated in contexts relevant to cancer biology and neurodevelopmental phenotypes.
ZSWIM8 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZSWIM8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZSWIM8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZSWIM8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZSWIM8 protein expression.
This CRISPR knockout system enables efficient generation of ZSWIM8-deficient cell models for investigation of ZSWIM8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZSWIM8 exon(s) critical for ZSWIM8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZSWIM8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZSWIM8 CRISPR/Cas9 KO Plasmid (h) and ZSWIM8 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZSWIM8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZSWIM8 HDR Plasmid (h) and ZSWIM8 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZSWIM8 homology arms to support homology-directed repair at defined ZSWIM8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.