ZRANB2 Antibody (B-5): sc-514200
- ZRANB2 Antibody (B-5) is a mouse monoclonal IgG2a κ ZRANB2 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 28-102 mapping near the N-terminus of ZRANB2 of human origin
- ZRANB2 Antibody (B-5) is recommended for detection of ZRANB2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ZRANB2 Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-514200 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for ZRANB2 Antibody (B-5) for WB applications. These reagents are now offered in bundles with ZRANB2 Antibody (B-5) (see ordering information below).
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ZRANB2 Antibody (B-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects ZRANB2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ZRANB2 Antibody (B-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZRANB2, also known as Zinc finger Ran-binding domain-containing protein 2, is a 330 amino acid protein that plays a crucial role in RNA processing as a splicing factor, specifically involved in the alternative splicing of Tra-2β (transformer-2 beta) transcripts. This function is vital for regulating gene expression and ensuring the production of diverse protein isoforms, which can significantly impact cellular functions and responses. ZRANB2 is localized in the nucleus and contains two RanBP2-type zinc fingers that facilitate RNA-binding activity, highlighting its importance in post-transcriptional regulation. Additionally, ZRANB2 has two isoforms, ZIS-1 and ZIS-2, generated through alternative splicing, and ZIS-2 can be phosphorylated by ATM or ATR in response to DNA damage, indicating its potential role in cellular stress responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ZRANB2 Antibody (B-5) References:
- Chromosome localization and characterization of the mouse and human zinc finger protein 265 gene. | Adams, DJ., et al. 2000. Cytogenet Cell Genet. 88: 68-73. PMID: 10773668
- ZNF265--a novel spliceosomal protein able to induce alternative splicing. | Adams, DJ., et al. 2001. J Cell Biol. 154: 25-32. PMID: 11448987
- The structure of the zinc finger domain from human splicing factor ZNF265 fold. | Plambeck, CA., et al. 2003. J Biol Chem. 278: 22805-11. PMID: 12657633
- XE7: a novel splicing factor that interacts with ASF/SF2 and ZNF265. | Mangs, AH., et al. 2006. Nucleic Acids Res. 34: 4976-86. PMID: 16982639
- Identification, characterization and mapping of the human ZIS (zinc-finger, splicing) gene. | Nakano, M., et al. 1998. Gene. 225: 59-65. PMID: 9931435
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZRANB2 Antibody (B-5) | sc-514200 | 200 µg/ml | $316.00 | |||
ZRANB2 Antibody (B-5): m-IgG Fc BP-HRP Bundle | sc-526350 | 200 µg Ab; 10 µg BP | $354.00 | |||
ZRANB2 Antibody (B-5): m-IgG2a BP-HRP Bundle | sc-546599 | 200 µg Ab; 10 µg BP | $354.00 | |||
ZRANB2 Antibody (B-5) X | sc-514200 X | 200 µg/0.1 ml | $316.00 | |||
ZRANB2 Antibody (B-5) AC | sc-514200 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ZRANB2 Antibody (B-5) HRP | sc-514200 HRP | 200 µg/ml | $316.00 | |||
ZRANB2 Antibody (B-5) FITC | sc-514200 FITC | 200 µg/ml | $330.00 | |||
ZRANB2 Antibody (B-5) PE | sc-514200 PE | 200 µg/ml | $343.00 | |||
ZRANB2 Antibody (B-5) Alexa Fluor® 488 | sc-514200 AF488 | 200 µg/ml | $357.00 | |||
ZRANB2 Antibody (B-5) Alexa Fluor® 546 | sc-514200 AF546 | 200 µg/ml | $357.00 | |||
ZRANB2 Antibody (B-5) Alexa Fluor® 594 | sc-514200 AF594 | 200 µg/ml | $357.00 | |||
ZRANB2 Antibody (B-5) Alexa Fluor® 647 | sc-514200 AF647 | 200 µg/ml | $357.00 | |||
ZRANB2 Antibody (B-5) Alexa Fluor® 680 | sc-514200 AF680 | 200 µg/ml | $357.00 | |||
ZRANB2 Antibody (B-5) Alexa Fluor® 790 | sc-514200 AF790 | 200 µg/ml | $357.00 |