ZP2 Antibody (IE-3): sc-32752

ZP2 Antibody (IE-3) is a rat monoclonal IgG2a antibody that detects ZP2 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-ZP2 antibody (IE-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZP2 protein plays a crucial role in the fertilization process, acting as a secondary sperm receptor that binds sperm only after the sperm acrosome reaction has been induced. This interaction facilitates the initial binding of sperm to the oocyte, which is essential for successful fertilization. The zona pellucida, a glycoprotein matrix surrounding the oocyte, contains ZP2 along with ZP1 and ZP3, and during this process ZP2 undergoes proteolytic cleavage, which is a significant post-translational modification that alters its function. Understanding the dynamics of ZP2 and its interactions is important for elucidating the mechanisms of fertilization and potential fertility treatments.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ZP2 Antibody (IE-3) References:

1. A family of genes encoding zona pellucida (ZP) domain proteins is expressed in various epithelial tissues during Drosophila embryogenesis.  |  Jaźwińska, A. and Affolter, M. 2004. Gene Expr Patterns. 4: 413-21. PMID: 15183308
2. Egg-sperm interactions at fertilization in mammals.  |  Wassarman, PM., et al. 2004. Eur J Obstet Gynecol Reprod Biol. 115 Suppl 1: S57-60. PMID: 15196717
3. Mouse zona pellucida genes and glycoproteins.  |  Wassarman, PM., et al. 2004. Cytogenet Genome Res. 105: 228-34. PMID: 15237211
4. Zona pellucida glycoproteins based immunocontraceptive vaccines: strategies for development and their applications.  |  Gupta, SK., et al. 2003. Indian J Exp Biol. 41: 682-93. PMID: 15255371
5. Biology of mammalian fertilization: role of the zona pellucida.  |  Dean, J. 1992. J Clin Invest. 89: 1055-9. PMID: 1556174
6. Zona pellucida domain proteins.  |  Jovine, L., et al. 2005. Annu Rev Biochem. 74: 83-114. PMID: 15952882
7. Gene knockouts that cause female infertility: search for novel contraceptive targets.  |  Naz, RK. and Rajesh, C. 2005. Front Biosci. 10: 2447-59. PMID: 15970507
8. Oocyte-specific expression of mouse Zp-2: developmental regulation of the zona pellucida genes.  |  Liang, LF., et al. 1990. Mol Cell Biol. 10: 1507-15. PMID: 1690843
9. POM-ZP3, a bipartite transcript derived from human ZP3 and a POM121 homologue.  |  Kipersztok, S., et al. 1995. Genomics. 25: 354-9. PMID: 7789967

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ZP2 Antibody (IE-3) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-ZP2 Antibody (IE-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ZP2 Antibody (IE-3) Specifications

Alternate Product Name: ZP2

Product Clone Name: IE-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids:

Epitope Target: ZP2

Epitope Species: rat

Product Reactivity/Detection: ZP2

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for ZP2 Antibody (IE-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred ZP2 antibody is ZP2 Antibody (C-7): sc-390422.