ZNF774 Antibody (E-2): sc-515778
- ZNF774 Antibody (E-2) is a mouse monoclonal IgG2a κ ZNF774 antibody provided at 200 µg/ml
- raised against amino acids 1-150 mapping at the N-terminus of ZNF774 of human origin
- ZNF774 Antibody (E-2) is recommended for detection of ZNF774 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ZNF774 Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ZNF774 Antibody (E-2) for WB applications. These reagents are now offered in bundles with ZNF774 Antibody (E-2) (see ordering information below).
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ZNF774 Antibody (E-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects ZNF774 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ZNF774 Antibody (E-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZNF774 consists of 483 amino acids and is characterized by 12 C2H2-type zinc fingers and a KRAB domain, which are crucial for transcriptional regulation. Located primarily in the nucleus, ZNF774 belongs to the Krüppel C2H2-type zinc-finger protein family, known for DNA binding and gene expression modulation. The KRAB domain of ZNF774 interacts with KAP1, a corepressor that recruits histone-modifying proteins, influencing chromatin structure and gene accessibility. This interaction regulates genes associated with various developmental and neurological disorders, as defects in genes located on human chromosome 15, where ZNF774 is encoded, are linked to conditions such as Angelman syndrome and Prader-Willi syndrome. ZNF774′s ability to modulate transcriptional activity maintains cellular homeostasis and regulates developmental processes, making anti-ZNF774 antibody (E-2) valuable for studying gene regulation and associated pathologies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ZNF774 Antibody (E-2) References:
- Clustering of C2-H2 zinc finger motif sequences within telomeric and fragile site regions of human chromosomes. | Lichter, P., et al. 1992. Genomics. 13: 999-1007. PMID: 1505991
- A family of human zinc finger proteins that bind methylated DNA and repress transcription. | Filion, GJ., et al. 2006. Mol Cell Biol. 26: 169-81. PMID: 16354688
- A comprehensive catalog of human KRAB-associated zinc finger genes: insights into the evolutionary history of a large family of transcriptional repressors. | Huntley, S., et al. 2006. Genome Res. 16: 669-77. PMID: 16606702
- [Progress in the study of KRAB zinc finger protein]. | Tian, CY., et al. 2006. Yi Chuan. 28: 1451-6. PMID: 17098717
- Diagnosis and management of patients with Marfan syndrome. | Midla, GS. 2008. JAAPA. 21: 21-5. PMID: 18323350
- Angelman syndrome: current understanding and research prospects. | Dan, B. 2009. Epilepsia. 50: 2331-9. PMID: 19874386
- The C15orf2 gene in the Prader-Willi syndrome region is subject to genomic imprinting and positive selection. | Wawrzik, M., et al. 2010. Neurogenetics. 11: 153-61. PMID: 20020165
- Tyrosinemia type 1 and Angelman syndrome due to paternal uniparental isodisomy 15. | Ferrer-Bolufer, I., et al. 2009. J Inherit Metab Dis. 32 Suppl 1: S349-53. PMID: 20033293
- Neuropsychiatric aspects of adult-onset Tay-Sachs disease: two case reports with several new findings. | Hurowitz, GI., et al. 1993. J Neuropsychiatry Clin Neurosci. 5: 30-6. PMID: 8428133
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZNF774 Antibody (E-2) | sc-515778 | 200 µg/ml | $322.00 | |||
ZNF774 Antibody (E-2): m-IgGκ BP-HRP Bundle | sc-525211 | 200 µg Ab, 40 µg BP | $361.00 | |||
ZNF774 Antibody (E-2): m-IgG2a BP-HRP Bundle | sc-546179 | 200 µg Ab; 10 µg BP | $361.00 | |||
ZNF774 Antibody (E-2) AC | sc-515778 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ZNF774 Antibody (E-2) HRP | sc-515778 HRP | 200 µg/ml | $322.00 | |||
ZNF774 Antibody (E-2) FITC | sc-515778 FITC | 200 µg/ml | $336.00 | |||
ZNF774 Antibody (E-2) PE | sc-515778 PE | 200 µg/ml | $349.00 | |||
ZNF774 Antibody (E-2) Alexa Fluor® 488 | sc-515778 AF488 | 200 µg/ml | $364.00 | |||
ZNF774 Antibody (E-2) Alexa Fluor® 546 | sc-515778 AF546 | 200 µg/ml | $364.00 | |||
ZNF774 Antibody (E-2) Alexa Fluor® 594 | sc-515778 AF594 | 200 µg/ml | $364.00 | |||
ZNF774 Antibody (E-2) Alexa Fluor® 647 | sc-515778 AF647 | 200 µg/ml | $364.00 | |||
ZNF774 Antibody (E-2) Alexa Fluor® 680 | sc-515778 AF680 | 200 µg/ml | $364.00 | |||
ZNF774 Antibody (E-2) Alexa Fluor® 790 | sc-515778 AF790 | 200 µg/ml | $364.00 |